Method for increasing content of mogroside V in suspension cells of grosvenor momordica fruit
A technology of mogroside and suspension cells, which is applied in the biological field, can solve the problems of yeast or methyl jasmonate in the suspension cell culture system of monk fruit, and meet the needs of market production, fast cell growth, and beneficial to the ecological environment The effect of protection
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Embodiment 1
[0027] (1) Get the mature seed embryo of Luo Han Guo, in containing sucrose 30g / L, 6-benzylaminoadenine 1.0mg / L, naphthalene acetic acid 0.5mg / L, inositol 100mg / L, agar 4.6g / L, pH range is Subculture on the MS semi-solid medium of 5.5-6.0, select fresh embryogenic callus that has been subcultured for 4 times and has vigorous growth, loose texture, stable and uniform state;
[0028](2) The fresh embryogenic callus obtained in step (1) was transferred to the inoculum containing 30 g / L of sucrose, 1.0 mg / L of 6-benzylaminoadenine, 0.5 mg / L of naphthaleneacetic acid, muscle Alcohol 100mg / L, pH range 5.5-6.5 MS liquid medium, and cultivated in a shaker with a rotation speed of 150r / min, a culture temperature of 25±2°C, a light intensity of 4000lux, and a light time of 12h / d , to obtain the first-generation Luo Han Guo suspension cell system;
[0029] (3) Cultivate mature Luo Han Guo suspension cells with the first-generation Luo Han Guo suspension cell system obtained in step (2);...
Embodiment 2
[0032] (1) Get the mature seed embryo of Luo Han Guo, in containing sucrose 30g / L, 6-benzylaminoadenine 1.0mg / L, naphthalene acetic acid 0.5mg / L, inositol 100mg / L, agar 4.6g / L, pH range is Subculture on the MS semi-solid medium of 5.5-6.0, select fresh embryogenic callus that has been subcultured for 3 times and has vigorous growth, loose texture, stable and uniform state;
[0033] (2) The fresh embryogenic callus that step (1) obtains is transferred to containing sucrose 30g / L, 6-benzylaminoadenine 1.0mg / L, naphthaleneacetic acid 0.5mg with the inoculum size 100g / L (fresh weight). / L, inositol 100mg / L, pH range of 5.5-6.5 in MS liquid medium, and at a rotation speed of 150r / min, a culture temperature of 25±2°C, a light intensity of 4000lux, and a light time of 12h / d. Cultivate in a shaker to obtain the first generation of Luo Han Guo suspension cell system;
[0034] (3) Cultivate mature Luo Han Guo suspension cells with the first-generation Luo Han Guo suspension cell system...
Embodiment 3
[0037] (1) Get the mature seed embryo of Luo Han Guo, in containing sucrose 30g / L, 6-benzylaminoadenine 1.0mg / L, naphthalene acetic acid 0.5mg / L, inositol 100mg / L, agar 4.6g / L, pH range is Subculture on the MS semi-solid medium of 5.5-6.0, select fresh embryogenic callus that has been subcultured for 5 times and has vigorous growth, loose texture, stable and uniform state;
[0038] (2) The fresh embryogenic callus that step (1) obtains is transferred to containing sucrose 30g / L, 6-benzylaminoadenine 1.0mg / L, naphthaleneacetic acid 0.5mg with the inoculum size 100g / L (fresh weight). / L, inositol 100mg / L, pH range of 5.5-6.5 in MS liquid medium, and at a rotation speed of 150r / min, a culture temperature of 25±2°C, a light intensity of 4000lux, and a light time of 12h / d. Cultivate in a shaker to obtain the first generation of Luo Han Guo suspension cell system;
[0039] (3) The first-generation Luo Han Guo suspension cell system obtained in step (2) was used to cultivate mature ...
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