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A method for increasing the content of mogroside V in suspension cells of Luo Han Guo

A mogroside and suspension cell technology, applied in the biological field, can solve problems such as yeast or methyl jasmonate treatment of a suspension cell culture system of Monk fruit, and achieve the effects of satisfying market-oriented production needs, saving land resources, and shortening the culture cycle time.

Active Publication Date: 2020-03-24
GUILIN NATURAL INGREDIENTS CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no report on the treatment of Luo Han Guo suspension cell culture system with yeast or methyl jasmonate

Method used

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  • A method for increasing the content of mogroside V in suspension cells of Luo Han Guo
  • A method for increasing the content of mogroside V in suspension cells of Luo Han Guo

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] (1) Get the mature seed embryo of Luo Han Guo, in containing sucrose 30g / L, 6-benzylaminoadenine 1.0mg / L, naphthalene acetic acid 0.5mg / L, inositol 100mg / L, agar 4.6g / L, pH range is Subculture on the MS semi-solid medium of 5.5-6.0, select fresh embryogenic callus that has been subcultured for 4 times and has vigorous growth, loose texture, stable and uniform state;

[0028](2) The fresh embryogenic callus obtained in step (1) was transferred to the inoculum containing 30 g / L of sucrose, 1.0 mg / L of 6-benzylaminoadenine, 0.5 mg / L of naphthaleneacetic acid, muscle Alcohol 100mg / L, pH range 5.5-6.5 MS liquid medium, and cultivated in a shaker with a rotation speed of 150r / min, a culture temperature of 25±2°C, a light intensity of 4000lux, and a light time of 12h / d , to obtain the first-generation Luo Han Guo suspension cell system;

[0029] (3) Cultivate mature Luo Han Guo suspension cells with the first-generation Luo Han Guo suspension cell system obtained in step (2);...

Embodiment 2

[0032] (1) Get the mature seed embryo of Luo Han Guo, in containing sucrose 30g / L, 6-benzylaminoadenine 1.0mg / L, naphthalene acetic acid 0.5mg / L, inositol 100mg / L, agar 4.6g / L, pH range is Subculture on the MS semi-solid medium of 5.5-6.0, select fresh embryogenic callus that has been subcultured for 3 times and has vigorous growth, loose texture, stable and uniform state;

[0033] (2) The fresh embryogenic callus that step (1) obtains is transferred to containing sucrose 30g / L, 6-benzylaminoadenine 1.0mg / L, naphthaleneacetic acid 0.5mg with the inoculum size 100g / L (fresh weight). / L, inositol 100mg / L, pH range of 5.5-6.5 in MS liquid medium, and at a rotation speed of 150r / min, a culture temperature of 25±2°C, a light intensity of 4000lux, and a light time of 12h / d. Cultivate in a shaker to obtain the first generation of Luo Han Guo suspension cell system;

[0034] (3) Cultivate mature Luo Han Guo suspension cells with the first-generation Luo Han Guo suspension cell system...

Embodiment 3

[0037] (1) Get the mature seed embryo of Luo Han Guo, in containing sucrose 30g / L, 6-benzylaminoadenine 1.0mg / L, naphthalene acetic acid 0.5mg / L, inositol 100mg / L, agar 4.6g / L, pH range is Subculture on the MS semi-solid medium of 5.5-6.0, select fresh embryogenic callus that has been subcultured for 5 times and has vigorous growth, loose texture, stable and uniform state;

[0038] (2) The fresh embryogenic callus that step (1) obtains is transferred to containing sucrose 30g / L, 6-benzylaminoadenine 1.0mg / L, naphthaleneacetic acid 0.5mg with the inoculum size 100g / L (fresh weight). / L, inositol 100mg / L, pH range of 5.5-6.5 in MS liquid medium, and at a rotation speed of 150r / min, a culture temperature of 25±2°C, a light intensity of 4000lux, and a light time of 12h / d. Cultivate in a shaker to obtain the first generation of Luo Han Guo suspension cell system;

[0039] (3) The first-generation Luo Han Guo suspension cell system obtained in step (2) was used to cultivate mature ...

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Abstract

The invention relates to a method for increasing the content of mogroside V in the suspension cells of grosvenor momordica fruit. According to the method, a yeast inducer with a concentration of 5 to200 mg / L and methyl jasmonate with a concentration of 50 to 260 mg / L are separately added in the 5th day to the 11th day of a culture period of the suspension cells of grosvenor momordica fruit, so the synthesis rate and yield of mogroside V are increased while the secretion of mogroside II is reduced. The method of the invention is beneficial for the market-oriented mass production and quality control and management of mogroside V.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for increasing the content of mogroside V in mogroside V in mogroside suspension cells. Background technique [0002] Mogroside V, also known as mogroside V, is the main sweet component of Siraitiagrosvenori in the Cucurbitaceae family, and its sweetness is 350 times that of sucrose. At present, seven mogrosides have been isolated and identified from Luo Han Guo, among which mogroside V and simonoside I have the strongest sweetness. Mogroside Ⅴ has medicinal effects such as clearing away heat and moistening the lungs, soothing the throat, relaxing the bowels, etc. At the same time, Mogroside Ⅴ also has the characteristics of novel taste and pure taste, which makes it deeply loved by people. It is the most suitable sweetener and health care product for special groups such as patients with diabetes, high blood pressure, and diabetes, and has a wide range of application values...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/04C12N5/02C12P33/20
CPCC12N5/04C12N2500/30C12N2500/34C12N2500/35C12N2500/40C12N2500/74C12N2501/999C12P33/20
Inventor 宋云飞杨文国李元元郭美锦李佳瑞王泽建庄英萍
Owner GUILIN NATURAL INGREDIENTS CORP
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