Method for fermenting chymosin by using nisin fermentation wastewater as partial nitrogen source
A technology of nisin and fermentation waste liquid, applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of nisin pollution of the environment and the high cost of rennet, reduce pollution, cost reduction effect
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specific Embodiment approach 1
[0009] Specific embodiment one: the present embodiment utilizes nisin fermented waste liquid to make the method for partial nitrogen source fermentation to produce chymosin, carry out according to the following steps: 1, the preparation of culture medium: NaH 2 PO 4 、Na 2 HPO 4 , glucose, high-temperature amylase, Tween 80, corn flour, corn starch, nisin fermentation waste liquid and soybean tissue protein were dissolved in water to prepare a medium, in which NaH 2 PO 4 : 3.25g / L, Na 2 HPO 4 : 3.11g / L, glucose: 2.14g / L, high temperature amylase: 20g / L, Tween 80: 0.13g / L, corn flour: 30~80g / L, corn starch: 0~30g / L, lactic acid chain Mycocin fermentation waste liquid: 150-700g / L, soybean textured protein: 0-20g / L; use 5%-6% sodium hydroxide to adjust the pH to 6.7-6.8, and sterilize at 0.14MPa pressure for 30min 2. The preparation of Rhizomucor miehei seed liquid: inoculate the inoculum of Rhizomucor miehei into the seed culture medium with an inoculum of 4% by volume, and...
specific Embodiment approach 2
[0012] Embodiment 2: This embodiment differs from Embodiment 1 in that: the concentration of corn flour added in Step 1 is 35-70 g / L. Others are the same as in the first embodiment.
specific Embodiment approach 3
[0013] Embodiment 3: The difference between this embodiment and Embodiment 1 or 2 is that the concentration of corn flour added in Step 1 is 50-60 g / L. Others are the same as in the first or second embodiment.
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