125 results about "Stenotrophomonas sp." patented technology

The invention discloses a preparation method of a composite microbial inoculant for degrading aflatoxin B1 and a composite microbial inoculant prepared by the method. The method comprises the following steps: respectively culturing pseudomonad, Flavobacterium, Rhodococcus, Stenotrophomonas and Bacillus to obtain a pseudomonad solution, a Flavobacterium solution, a Rhodococcus solution, a Stenotrophomonas solution and a Bacillus solution; mixing the pseudomonad solution, Flavobacterium solution, Rhodococcus solution, Stenotrophomonas solution and Bacillus solution to obtain a composite bacterium solution; and putting the composite bacterium solution into a composite bacterium culture medium, and carrying out mixed culture fermentation to obtain the composite microbial inoculant for degrading aflatoxin B1. The composite microbial inoculant can efficiently degrade aflatoxin B1, and has the advantages of low cost and no secondary pollution.

The invention discloses a stenotrophomonas acidaminiphila and an application in control of apple tree canker thereof, and belongs to the technical field of plant disease biological control. A strain of stenotrophomonas acidaminiphila BJ1 used for apple fungal disease biological control is screened from rhizosphere soil of a commercial apple grove in Xixia, Shandong, is preserved in China center for type culture collection with a preservation number of CCTCC NO: M 2011475. The microbial agent of the invention is prepared by the following steps of: 1) preparation of a medium, 2) activation of the BJ1 strain, 3) liquid culture of the BJ1 strain, 4) preparation of a BJ1 biological control preparation, and 5) dilution. The stenotrophomonas acidaminiphila BJ1 strain of the invention has obvious control effect on apple tree canker, has stable control effect, is environment-friendly, is simple in fermentation process for large-scale production, and low in production cost.

A microorganism preparation method relates to the microorganism of stenotrophomonas maltrophilia of stenotrophomonas utilized by CCTCC NoM 204024 of its fermentation and decomposition or its cells or zymes decomposite substrates validamycin or validamycin imide to produce validamy enamine and validemy amine. Composition of the culture media is(weight/volume): validamycin 0.5-20.0%,(NH4)2 SO4 0.5%-10.0, KCL 0.5%-5.0%, Na2HPO4, 12H2 00.1%-10.0%, Na2H2PO4 2H2O 0.1-5.0%, MgSO4 0.01%-1.0% matched by running water, fermenting temperature: 20deg.C-40-deg.C initial PH:6.0-8.0 for 1-180h by ionic exchanges, chromatography and purify, the two products are finished.

The invention aims at providing an astaxanthin esterase production strain and application of the astaxanthin esterase production strain in preparation of free astaxanthin. The strain for producing lipase and esterase is stenotrophomonas and stored with the number of CGMCC NO.10672. The stenotrophomonas strain can be used for producing free astaxanthin by efficiently utilizing an organic solvent extract of haematococcus pluvialis oil and/ or aquatic animal (such as shrimp and crab); the strain can effectively degrade astaxanthin ester to prepare free astaxanthin; in addition, the experiment shows that the quantity of added tween 80 in fermenting liquid, inoculation amount, initial pH of fermenting liquid and fementing time condition can be determined; a method for efficiently preparing the free astaxanthin is provided.

The object of the present invention is a composition of bacterial strains which may comprise Stenotrophomonas sp. strain 2L, Stenotrophomonas sp. strain 5L, Stenotrophomonas sp. strain 6L, Stenotrophomonas sp. strain 3N, Achromobacter sp. strain 4P, Arthrobacter sp. strain 1N, Brevundimonas sp. strain 2N, Brevundimonas sp. strain 5N, Brevundimonas sp. strain 6N, Pseudomonas sp. strain 3G, and Pseudomonas sp. strain 4, deposited under the number KKP 2041p (IAFB Collection of Industrial Microorganisms—Institute of Agricultural and Food Biotechnology in Warsaw), a bioremediation vaccine (bioremediation mixture) which may comprise the composition of these strains, the use of the vaccine in the removal of contaminants from the soil, and the method for the treatment of contaminated soil.

The invention provides a strain of stenotrophomonas sp., which is named as stenotrophomonas F6, and has a preservation number of CGMCC No.6547. Two active algicidal substances, namely cyclo(glycine-proline) and hydroquinone, are separated from the metabolism products of the stenotrophomonas F6, and then the substances are purified and identified. The half effective concentration of the cyclo(glycine-proline) on inhibiting microcystis aeruginosa 9110 is 9.5 mg/L, and the cyclo(glycine-proline) has no inhibiting effect on synechococcus BN60. The half effective concentrations of hydroquinone on inhibiting microcystis aeruginosa 9110 and synechococcus BN60 are 0.96 mg/L and 5.6 mg/L. The stenotrophomonas F6 and the active algicidal substances (cyclo(glycine-proline) and hydroquinone) can be applied to the development and production of novel algicide, and finally are applied to the control of cyanobacterial bloom in fresh water.

The invention discloses stenotrophomonas (sp.) and application thereof. The stenotrophomonas (sp.) provided by the invention is stenotrophomonas (sp.) FJSB3 with the collection number of CGMCC No.4344. As an experiment proves, the stenotrophomonas (sp.) FJSB3 with the collection number of CGMCC No.4344 can degrade pure Bt proteins and the Bt proteins in trans-Bt rice straw. The stenotrophomonas (sp.) strain has good application prospect in the fields of degradation of Bt proteins in soil and improvement on the soil environment.

Recalcitrant chemical oxygen demand (COD) of a liquid is reduced in a water treatment system. The method includes pretreating the liquid in a pretreatment unit to remove indigenous bacteria or microbes to a population level below which the indigenous organisms can interfere with the screened and externally introduced microorganisms. The liquid is then provided to a reactor that has a filter bed formed with a carrier material. Special microbes are screened and used to colonize the carrier material to remove recalcitrant COD. A biofilm is cultured on the surface of the carrier material to immobilize the screened microbes in the reactor. The method further includes percolating the liquid from the pretreatment unit through the filter bed colonized with the screened microbes to degrade at least part of the recalcitrant COD under aerobic conditions. In one embodiment, the filter is formed with biological granular activated carbon (GAC) as the carrier material and the screened microbes comprise at least one microbial species selected from the group consisting of Bacillus, Comamonas, Arthrobacter, Micrococcus, Pseudomonas, Pediococcus, Achromobacter, Flavobacterium, Mycobacterium, Rhodanobacter, Stenotrophomonas and Yeast.

The invention discloses a microbial agent for degrading fomesafen and a preparation method and application thereof, and belongs to the field of microorganisms. The microbial agent aims at solving theproblem that currently, there is no microbial agent for effectively degrading fomesafen; bacteria adopted in the microcapsule microbial agent are mixed bacteria of bacillus velezensis FB11 and stenotrophomonas sp. FB14, the preservation numbers of the bacteria are CGMCC No.17732 and CGMCC No.17731 respectively, the preservation dates are both May 8th, 2019, and the preservation address is BuildingNo.3, NO.1 West Beichen Road, Chaoyang District, Beijing. After the two strains are mixed, auxiliary materials are added to prepare the microbial agent. The microbial agent is used for degrading theherbicide fomesafen. The mixed strains adopted for the microbial agent have a remarkable degradation effect on the herbicide fomesafen in soil.

The invention discloses pyrethroid pesticide degrading bacteria and a bactericide thereof, belonging to the field of biological high-tech. The adopted strain ZS-S-01 is identified as Stenotrophomonas sp. with the preservation number of CCTCC M 2010095, wherein, the Genbank registry number of the 16S rDNA of the strain is HM016874. The main biological characteristics are as follows: the strain is Gram-negative and has multiple polar flagellum and cells in a short rod shape; and the bacterial colony is milky white and slight yellowish, thicker and non-transparent, the diameter is 0.5mm-1mm, the edge is regular and orderly, the middle part is slightly protruding, and the surface is smooth and bright without wrinkles. The bactericide of the invention has the advantages of low production cost, convenient use and good degradation effect, thus being applicable to the national fruit and vegetable production and export bases or places with green food brand marks at a large area. The bactericide is of great significance to promoting production of nuisance-free vegetables and green food.

The invention provides stenotrophomonassp. H-4 and a preparation method of degrading enzyme preparation thereof as well as application. The stenotrophomonassp. H-4 has been conserved in the conservation unit specified by the State Intellectual Property Office; the conservation date is February 1, 2013; the name of the conservation unit is China General Microbiological Culture Collection Center; and the conservation number is CGMCC No.7274. The stenotrophomonassp. H-4 can be grown on a culture medium with chlorothalonil as the unique carbon source and energy, and after being cultivated for 7 days, the degradation rate of the stenotrophomonassp. H-4 reaches 83.6%; the enzyme activity is 3.28 U and the degradation rate of the degrading enzyme to the chlorothalonil is 86% when the extraction conditions for crude enzyme in the stenotrophomonassp. H-4 are as follows: ultrasonic powder: 30 W; and ultrasonic time: 5 minutes. Obviously, the degrading enzyme preparation provided by the invention has bright application prospect in clearing away residual pesticides on vegetables and fruits.

The invention relates to a degrading strain BJ1 capable of efficiently degrading a bactericide-chlorothalonil, and application of the degrading strain in greenhouse soil environment, belonging to thetechnical field of biodegradation. The degrading strain is identified as stenotrophomonas acidaminiphila and has a strain preservation number of CCTCC No. M2011475. The strain can use the chlorothalonil as an only carbon source; after the degrading strain is cultured in an inorganic salt culture solution for seven days, the degradation rate of the 50mg/L chlorothalonil can reach 93.2%. In the high-temperature and high-humidity greenhouse environment, the application of strain BJ1 can promote the degradation of the chlorothalonil in the soil, and the degradation rate can be increased by 45.6%;furthermore, the strain BJ1 can significantly reduce chlorothalonil-induced soil genotoxicity. The degrading stain is mainly used for the biological purification of chlorothalonil residues in the soil, especially in the soil in the greenhouse environment, thus reducing the harm, caused by the chlorothalonil, to the environment, and realizing functions of repairing the chlorothalonil-contaminated soil and protecting the ecological environment.

The invention belongs to the technical field of microorganism and especially relates to a stenotrophomonas FQ1 strain and its screening method and use. The stenotrophomonas FQ1 strain has the accession number of CGMCC No.8272. A use method of the stenotrophomonas FQ1 strain for enteromorpha prolifera enzymolysis comprises the following steps of carrying out activation culture on the frozen stenotrophomonas FQ1 strain by a LB slant culture medium, picking a full ring of the culture, putting it into a LB liquid culture medium, carrying out shaking culture for 24-28h, taking 1-2mL of the bacterial solution, putting the bacterial solution into a sterilized cellulose liquid fermentation medium, carrying out shaking culture for 5-7 days, and carrying out low-temperature centrifugation on the fermentation broth and collecting a supernatant as a crude enzyme liquid, or filtering the fermentation broth and collecting the filtrate as a crude enzyme liquid; and carrying out a process of reaction of the crude enzyme liquid and clean enteromorpha prolifera powder at a temperature of 45-55 DEG C. The stenotrophomonas FQ1 strain has a cellulase production capability and can effectively degrade cellulose materials in enteromorpha prolifera into reducing sugar. A production cost is low, production equipment is simple, reaction conditions are mild, and the stenotrophomonas FQ1 strain is suitable for large-scale industrial production.

The invention belongs to the field of environment pollution bio-remediation, and particularly relates to a siderophore high-yielding strain preparation and an application of the same in remediation of heavy metal contaminated soil. The siderophore high-yielding strain preparation contains a combination of Bacillus sp. bacteria S86, Pseudomonas sp. bacteria S17 and Stenotrophomonas rhizophila LD-6. The siderophore high-yielding strain preparation can be used for remediation of heavy metal contaminated soil of the farmland. The application is mainly used for reinforcing remediation of typical heavy metal contaminated soil of sweet sorghum.

The invention provides a degradable seedling growing pot with a function of promoting plant growth, a method for preparing the degradable seedling growing pot and application thereof. A seedling growing pot composition comprises mixed biological substrates and compound bacterial sludge. The mixed biological substrates comprise 50-80 parts of crop straw, 5-8 parts of sawdust, 5-10 parts of livestock and poultry manure, 2-5 parts of grass carbon, 2-5 parts of weathered coal and 3-6 parts of soil; the compound bacterial sludge comprises Azospirillum spp., Azobacter spp., Bacillus spp., Paenibacillus spp., Massilia spp., Stenotrophomonas spp. and Lysobacterspp. The degradable seedling growing pot, the method and the application have the advantages that the germination rates of plants can be increased by the degradable seedling growing pot prepared from the seedling growing pot composition, balanced nutrition required during growth can be supplied to seedlings, pathogenic microorganisms can be resisted, and accordingly the degradable seedling growing pot is favorable for strong seedlings.

The invention discloses a composite microbial agent, a preparation method and application thereof. The composite microbial agent comprises a stenotrophomonas rhizophila X1 and a pseudomonas L1. The preparation method of the composite microbial agent comprises the steps that (1) the stenotrophomonas rhizophila X1 and the pseudomonas L1 are subjected to pure culture correspondingly, and seed liquidof the two bacteria is correspondingly obtained; (2) the seed liquid of the stenotrophomonas rhizophila X1 and the seed liquid of the pseudomonas L1 are correspondingly centrifuged, and thalli of thetwo bacteria are correspondingly collected; and (3) the thallus of the stenotrophomonas rhizophila X1 and the thallus of the pseudomonas L1 are correspondingly resuspended with a buffer solution, resuspended bacterium liquid is mixed evenly, and the composite microbial agent is obtained. The composite microbial agent is applied to soil in which Cr (VI) and PAHs coexist, and can simultaneously remove the Cr (VI) and the PAHs, the removal rate of the Cr (VI) is as high as 88%, and the removal rate of the PAHs is as high as 55%.

The invention discloses a mixed microbial agent for preventing and controlling brown spot of tobaccos and a preparation method of the mixed microbial agent, and belongs to the technical field of biological pesticide. The mixed microbial agent comprises 15-25% of Achromobacter, 5-15% of Enterobacter sp., 5-15% of Ochrobactrum sp., 40-60% of Stenotrophomonas sp. and 5-15% of Bacillus subtilis. The preparation method of the mixed microbial agent comprises the step of preparing the composite microbial agent from the Achromobacter, Enterobacter sp., Ochrobactrum sp., Stenotrophomonas sp. and Bacillus subtilis at a volume ratio, and the application mode of the microbial agent comprises the steps: adopting spray application, and spraying the microbial agent to both sides of leaves. It is proved through experiments that the prepared microbial agent has a high inhibitory effect on pathogenic bacteria of tobacco brown spot, and a good disease prevention effect on tobacco brown spot is achieved;and the microbial agent has low cost, a good effect and environmental protection, and therefore good prospects for biopesticide development are achieved.

The invention relates to the technical field of microbial remediation of heavy metal chromium in environment, particularly to a microbial agent for hexavalent chromium pollution treatment. The effective component of the microbial agent is (stenotrophomonas acidaziphi) 4-1 or fermentation liquor of (stenotrophomonas acidaziphi) 4-1.

The invention relates to stenotrophomonas maltophilia sneb 42, which is capable of inducing soybeans to generate atrazine resistance when used for treating soybean seeds, a method for preparing the same and application thereof, and belongs to the technical field of microbial pesticides. The bacterial strain of sneb 42 was preserved in the General Microbiological Culture Collection Center of China Committee for Culture Collection of Microorganisms on February 1st, 2010. The preservation number is CGMCC No. 3620. The stenotrophomonas is prepared by liquid fermentation culture, and when metabolites of the stenotrophomonas are used for treating soybean seeds, the soybean seeds can generate the atrazine resistance while germinating. After the soybeans are treated by the metabolites of the stenotrophomonas, the soybeans can be co-planted with gramineae plants such as corns, and all plants can be weeded by using the uniform atrazine herbicide, so that the problem that the weeding problem in the intercropping between corns and soybeans is solved. The stenotrophomonas and the method have promising application and development prospects.

The invention discloses stenotrophomonas for promoting growth and development of Lilium spp and/or antagonizing pathogenic bacteria of the Lilium spp and application of the stenotrophomonas. The stenotrophomonas is stenotrophomonas rhizophila, a strain number of the stenotrophomonas rhizophila is G44, and a registration number of the stenotrophomonas rhizophila in the China General MicrobiologicalCulture Collection Center is CGMCC No.20185. The stenotrophomonas has the characteristics of producing siderophore, producing indoleacetic acid, producing ACC deaminase and the like for promoting plant growth, and the growth and development of Lilium spp plants can be obviously optimized by inoculating the stenotrophomonas to the Lilium spp plants, namely, the growth of overground parts and underground parts of the Lilium spp is promoted, the flowering time of the Lilium spp is advanced, the reproductive growth period of the Lilium spp is prolonged, and the fresh weight of bulbs of the Liliumspp is increased. Meanwhile, the strain has an obvious inhibition or antagonism effect on the pathogenic bacteria of the Lilium spp, such as botrytis cinerea of the Lilium spp.