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Stenotrophomonas for promoting growth and development of Lilium spp and/or antagonizing pathogenic bacteria of Lilium spp and application of stenotrophomonas

A technology for the growth and development of oligotrophomonas bacteria, applied in the field of microorganisms, can solve problems such as environmental pollution, destruction of ecological balance, food safety hazards, etc., and achieve good application prospects and the effect of growth optimization

Active Publication Date: 2021-04-02
BEIJING AGRO BIOTECH RES CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the prevention and control of lily diseases mainly adopts chemical control, but long-term use of chemical pesticides will not only cause environmental pollution and destroy ecological balance; Pathogenic bacteria develop drug resistance, affecting the control effect

Method used

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  • Stenotrophomonas for promoting growth and development of Lilium spp and/or antagonizing pathogenic bacteria of Lilium spp and application of stenotrophomonas
  • Stenotrophomonas for promoting growth and development of Lilium spp and/or antagonizing pathogenic bacteria of Lilium spp and application of stenotrophomonas
  • Stenotrophomonas for promoting growth and development of Lilium spp and/or antagonizing pathogenic bacteria of Lilium spp and application of stenotrophomonas

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Embodiment 1. Isolation and identification of bacterial strain G44

[0050] 1. Isolation of Strain G44

[0051] In November 2016, Chenxuqing (chenxuqing@baafs.net.cn) collected the rhizosphere soil samples of Lanzhou lily at Fangshan Lily Experimental Base of Beijing Academy of Agriculture and Forestry Sciences, separated the bacteria by plate dilution method, weighed 10g of the soil sample and placed it in 90mL of sterile water. 28°C, 180r / min shaking culture for 1 hour to prepare a soil sample suspension, absorb 1ml of the above soil sample suspension and add it to 9ml sterile water to make 10 -1 Dilution, continue to dilute to make 10 -2 、10 -3 、10 -4 、10 -5 、10 -6 0.1ml of each of the last four dilutions of the bacterial suspension was spread on the LB solid medium plate, and cultured statically at 28°C. 2 to 3 days after the colonies are formed, purify the strains on the LB solid medium plate by streaking. One of the isolated and purified strains was named G...

Embodiment 2

[0061] Embodiment two, bacterial strain G44 growth-promoting characteristic determination

[0062] 1. Determination of 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase activity of bacterial strain G44

[0063] The ACC deaminase activity assay was carried out on the bacterial strain G44 obtained in Example 1. The specific method is as follows: use 5ml TSB liquid medium, culture at 30°C for 24h, activate the bacterial strain G44, draw 0.5mL bacterial liquid into 60mL TSB medium, After expanded culture at 30°C for 24-48h, centrifuge at 8000rpm for 10min at 4°C, discard the supernatant, and collect the bacteria. Use 15ml of bacteria without (NH 4 ) 2 SO 4 The DF liquid medium was centrifuged and washed twice, the bacteria were resuspended in 24ml ADF medium (ACC final concentration 3mM), cultured at 30°C for 24h, centrifuged at 8000rpm at 4°C for 10min, discarded the supernatant, collected the bacteria, and recorded the bacteria weight. The bacteria were washed twice by ...

Embodiment 3

[0073] Embodiment three, lily growth-promoting greenhouse pot experiment and field experiment

[0074] The promotion effect of the strain G44 in Example 1 on the growth and development of the three lily varieties of Shuttle lily, Oklahoma lily and Lanzhou lily under greenhouse conditions and field conditions was determined through greenhouse pot experiments and field experiments.

[0075] 1. The root growth promotion experiment of strain G44 on Shuttle Lily greenhouse potted plants

[0076] Mix peat soil, perlite, and vermiculite in a volume ratio of 2:1:1 and put them into a 20cm x 20cm flowerpot. Sow 1 shuttle lily bulb in each pot. Pots were designed using a completely randomized design (CRD). Divided into two treatment groups: non-inoculated control group (CK) and inoculated strain G44 inoculated group (G44), each treatment had 3 replicates, and each replicate had 1 pot. Placed in a glass greenhouse at 26°C, cultivated under natural light, and watered regularly and quan...

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Abstract

The invention discloses stenotrophomonas for promoting growth and development of Lilium spp and / or antagonizing pathogenic bacteria of the Lilium spp and application of the stenotrophomonas. The stenotrophomonas is stenotrophomonas rhizophila, a strain number of the stenotrophomonas rhizophila is G44, and a registration number of the stenotrophomonas rhizophila in the China General MicrobiologicalCulture Collection Center is CGMCC No.20185. The stenotrophomonas has the characteristics of producing siderophore, producing indoleacetic acid, producing ACC deaminase and the like for promoting plant growth, and the growth and development of Lilium spp plants can be obviously optimized by inoculating the stenotrophomonas to the Lilium spp plants, namely, the growth of overground parts and underground parts of the Lilium spp is promoted, the flowering time of the Lilium spp is advanced, the reproductive growth period of the Lilium spp is prolonged, and the fresh weight of bulbs of the Liliumspp is increased. Meanwhile, the strain has an obvious inhibition or antagonism effect on the pathogenic bacteria of the Lilium spp, such as botrytis cinerea of the Lilium spp.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a Stenotrophomonas strain that promotes the growth and development of lily and / or antagonizes lily pathogenic bacteria and its application. Background technique [0002] Lilium (Lilium spp.) refers to the bulbous flower of Liliaceae (Liliaceae), which is a perennial herbaceous bulbous plant. Lily is one of the most popular fresh-cut flowers in the world, and it has high ornamental, edible and medicinal value. However, lily is a kind of plant susceptible to diseases. With the continuous expansion of cultivation area and continuous cropping years, the occurrence of lily diseases is becoming more and more serious, which has become the main factor restricting the development of lily industry. Among them, the lily gray mold caused by Botrytis spp. seriously restricts the cultivation and production of lily. Plants die early, which affects bulb yield. In addition, lily wilt (F...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/20A01P3/00A01P21/00C12R1/01
CPCA01N63/20
Inventor 高俊莲张秀海陈绪清孙雨晨杜运鹏薛静张铭芳杨凤萍
Owner BEIJING AGRO BIOTECH RES CENT
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