Siderophore high-yielding strain preparation and application of same in remediation of heavy metal contaminated soil

A bacterial preparation and siderophore technology, applied in the field of bioremediation of environmental pollution, can solve the problems of lack of strains, few strains, and limited strains with high siderophore production

Active Publication Date: 2017-02-22
ZHEJIANG FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, among the reported strains, siderophore high-yield strains are limited; strains with multiple advantages and characteristics (such as multiple heavy metal resistance and resistance to pathogenic fungi) are lacking; strains that can actually strengthen the ability of plants to absorb heavy metals in soil very few

Method used

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  • Siderophore high-yielding strain preparation and application of same in remediation of heavy metal contaminated soil
  • Siderophore high-yielding strain preparation and application of same in remediation of heavy metal contaminated soil
  • Siderophore high-yielding strain preparation and application of same in remediation of heavy metal contaminated soil

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1: Isolation of Pseudomonas sp.S17 and Bacillus sp.S86 and Siderophore Production Ability Test

[0030] 100g of soil in the experimental field of Zhejiang University of Science and Technology, Hangzhou, Zhejiang Province was collected on-site by the general five-point sampling method, packed in a sterile envelope, and brought back to the laboratory quickly.

[0031] General CAS detection solution and MSA liquid medium are based on the report of Chen Shaoxing et al. ) preparation. The solid detection plate is solidified by adding 5% CAS detection solution and 2% agar powder into MSA liquid medium.

[0032] Dilute the soil sample by 10-fold decreasing method, and spread it on one-fifth LB solid plate (that is, all nutrients are 1 / 5 of the general LB medium). Cultivate at 25°C. After colonies appear, pick out the colonies of different forms one by one with a sterile toothpick and transfer them to a new one-fifth LB solid plate until they are purified into single ...

Embodiment 2

[0038] Example 2: Antibiotic susceptibility test and pathogenic fungus antagonistic ability test of Pseudomonas sp.S17 and Bacillus sp.S86

[0039] The antibiotic susceptibility test adopts the filter paper method, and five commonly used antibiotics such as ampicillin, tetracycline, chloramphenicol, kanamycin and streptomycin are selected, and the antibacterial activity of each antibiotic filter paper on the siderophore-producing bacteria S17 and S86 plates is selected. The size of the ring diameter is used as a criterion for judging sensitive or resistant (resistant) drugs. The test results given in Table 4 and Table 5 show that the strains S17 and S86 are sensitive to the above-mentioned tested antibiotics in varying degrees.

[0040] Table 4. Antibiotic susceptibility test results of bacterial strain S17

[0041]

[0042] Note: If the diameter of the inhibition zone is larger than the upper limit of the moderately sensitive range, it is highly sensitive, and if it is sm...

Embodiment 3

[0051] Embodiment 3: The heavy metal minimum inhibitory concentration (MIC) test of Pseudomonas sp.S17 and Bacillus sp.S86

[0052] The design of the MIC experiment was carried out according to the research of Filali et al. (Filali BK, Taoufik J, Zeroual Y, Dzairi FZ, Talbi M, Blaghen M. Waste water bacterial isolates resistant to heavy metals and antibiotics. Current Microbiology, 2000, 41: 151-156), Single colonies of S17 and S86 of equal size and activation treatment were picked and placed in culture test tubes, and cultured at 30° C. with shaking at 160 r / min for 32 hours. The MIC value was determined according to the growth of the bacteria, and three replicates were set up in each group to test the tolerance of the strains to 12 metal ions. The results are shown in Table 7. The tested strains have multiple heavy metal tolerance (resistance) and excellent properties.

[0053] Table 7. MIC test results

[0054]

[0055]

[0056] Note: The data in the table is the av...

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Abstract

The invention belongs to the field of environment pollution bio-remediation, and particularly relates to a siderophore high-yielding strain preparation and an application of the same in remediation of heavy metal contaminated soil. The siderophore high-yielding strain preparation contains a combination of Bacillus sp. bacteria S86, Pseudomonas sp. bacteria S17 and Stenotrophomonas rhizophila LD-6. The siderophore high-yielding strain preparation can be used for remediation of heavy metal contaminated soil of the farmland. The application is mainly used for reinforcing remediation of typical heavy metal contaminated soil of sweet sorghum.

Description

technical field [0001] The invention belongs to the field of bioremediation of environmental pollution, in particular to a siderophore high-yield bacterial preparation and its application in the aspect of remediation of heavy metals in polluted soil. Background technique [0002] Heavy metal pollution in soil has become a global environmental problem, which has attracted the attention of the society and the public and needs to be solved urgently. The soil in many areas of my country (especially in East China) is polluted by heavy metals to varying degrees. The content of heavy metals such as cadmium, arsenic, copper, lead, and zinc in grains, vegetables, and fruits in many areas exceeds the standard or is close to the critical value, seriously endangering the health of the people. [0003] Soil heavy metal pollution control is imminent but difficult, mainly because heavy metals are not biodegradable like organic pollutants, and heavy metals have poor mobility in soil. Tradi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20B09C1/10C12R1/07C12R1/38C12R1/01
CPCB09C1/105C12N1/20
Inventor 虞方伯钱晓云单胜道管莉菠管泽华鲍雅莉付源
Owner ZHEJIANG FORESTRY UNIVERSITY
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