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Complex microbial inoculant for wastewater treatment and preparation method of complex microbial inoculant

A compound bacterial agent, wastewater treatment technology, applied in microorganism-based methods, biological water/sewage treatment, biochemical equipment and methods, etc., can solve the problems of poor reuse performance, low denitrification efficiency, etc. Nitrification effect, reducing ammonia nitrogen content, preventing microbial leakage

Active Publication Date: 2022-04-12
江苏利然环保科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In view of the deficiencies in the prior art, the purpose of the present invention is to provide a composite bacterial agent for wastewater treatment and its preparation method, to solve the technical problems of low denitrification efficiency and poor reusability of the traditional traditional biological denitrification technology

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0032] A preparation method for a composite bacterial agent for wastewater treatment, comprising the steps of:

[0033] (1) Inoculate Shewanella putrefaciens and Pseudomonas pseudoalcaligenes respectively in the liquid fermentation medium according to the inoculation amount of 10% by volume, and cultivate them at 25° C. for 18 hours to prepare Shewanella putrefacilis fermentation liquid respectively and Pseudomonas pseudoalcaligenes fermentation broth, the liquid fermentation medium includes the following components by weight: 8 parts of ammonium salt, 12 parts of potassium dihydrogen phosphate, 5 parts of magnesium chloride, 5 parts of sodium carbonate, 6 parts of glucose, trace elements 3 parts of liquid, 800 parts of deionized water, wherein the trace element liquid consists of the following raw materials in parts by weight: ZnSO 4 2.2g, CaCl 2 5.5g, Na 2 EDTA 63.70g, MnCl 2 4H 2 O5.06g, FeSO 4 ·7H 2 O 5.0g, CuSO 4 ·5H 2 O 1.57g, Na 2 MoO 4 4H 2 O 1.1g, CoCl 2...

Embodiment 2

[0042] A preparation method for a composite bacterial agent for wastewater treatment, comprising the steps of:

[0043] (1) Inoculate Shewanella putrefaciens and Pseudomonas pseudoalcaligenes respectively in the liquid fermentation medium according to the inoculation amount of 12% by volume, and cultivate them at 25° C. for 18 hours to prepare Shewanella putrefacilis fermentation liquid respectively and Pseudomonas pseudoalcaligenes fermentation broth, the liquid fermentation medium includes the following components by weight: 8 parts of ammonium salt, 12 parts of potassium dihydrogen phosphate, 5 parts of magnesium chloride, 5 parts of sodium carbonate, 6 parts of glucose, trace elements 3 parts of liquid, 800 parts of deionized water, wherein the trace element liquid consists of the following raw materials in parts by weight: ZnSO 4 2.2g, CaCl 2 5.5g, Na 2 EDTA 63.70g, MnCl 2 4H 2 O5.06g, FeSO 4 ·7H 2 O 5.0g, CuSO 4 ·5H 2 O 1.57g, Na 2 MoO 4 4H 2 O 1.1g, CoCl 2...

Embodiment 3

[0052] A preparation method for a composite bacterial agent for wastewater treatment, comprising the steps of:

[0053] (1) Inoculate Shewanella putrefaciens and Pseudomonas pseudoalcaligenes respectively in the liquid fermentation medium according to the inoculation amount of 15% by volume, and cultivate them at 25° C. for 18 hours to prepare Shewanella putrefacilis fermentation liquid respectively and Pseudomonas pseudoalcaligenes fermentation broth, the liquid fermentation medium includes the following components by weight: 8 parts of ammonium salt, 12 parts of potassium dihydrogen phosphate, 5 parts of magnesium chloride, 5 parts of sodium carbonate, 6 parts of glucose, trace elements 3 parts of liquid, 800 parts of deionized water, wherein the trace element liquid consists of the following raw materials in parts by weight: ZnSO 4 2.2g, CaCl 2 5.5g, Na 2 EDTA 63.70g, MnCl 2 4H 2 O5.06g, FeSO 4 ·7H 2 O 5.0g, CuSO 4 ·5H 2 O 1.57g, Na 2 MoO 4 4H 2 O 1.1g, CoCl 2...

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Abstract

The invention discloses a complex microbial inoculant for wastewater treatment and a preparation method thereof.The complex microbial inoculant comprises shewanella putrefaciens, pseudomonas pseudoalcaligenes and modified zeolite.Chitosan is introduced to the zeolite, the adsorption performance of the zeolite is improved, then composite modification is conducted, iron phosphate is introduced to the zeolite, and the adsorption performance of the zeolite is improved; the iron ions can be used as a coagulant and cooperate with chitosan to flocculate and settle ammonia nitrogen substances in sewage, the biological nitrification performance of the composite microbial inoculant is greatly improved, in addition, the iron ions can increase the content of organic polymers in bacterial biofilms, and the colloidal network structure of the organic polymers can aggregate cells together, so that the biological nitrification performance of the composite microbial inoculant is improved. Meanwhile, the biological membrane is adhered to the surface of the zeolite carrier, so that the connectivity between the zeolite carrier and microorganisms is increased, the leakage of the microorganisms is effectively prevented, and the complex microbial inoculant can keep a stable biological nitrification effect.

Description

technical field [0001] The invention relates to the technical field of sewage treatment, in particular to a composite bacterial agent for wastewater treatment and a preparation method thereof. Background technique [0002] Immobilized microorganism technology is a biotechnology developed from immobilized enzyme technology in biochemical industry in the 1960s. By chemical or physical means, free cells are positioned in a limited space area to keep them active and can be used repeatedly. In the late 1970s, with the increasingly serious water pollution, this technology was applied to the biological treatment of industrial wastewater due to its unique advantages. Compared with the traditional suspension biological treatment method, immobilized microorganism technology has the ability to improve the microbial Cell concentration, maintaining high-efficiency strains, high microbial density and less loss, fast response, strong poison resistance, simple processing equipment, etc., ha...

Claims

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Application Information

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IPC IPC(8): C12N11/14C12N11/10C12N1/20C02F3/34C01B39/02C12R1/02C12R1/38C02F101/16
Inventor 汪周启汪邦国
Owner 江苏利然环保科技有限公司
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