Waste water treatment agent and application thereof
A wastewater treatment agent and wastewater technology, applied in biological water/sewage treatment, water/sludge/sewage treatment, fixed on/in organic carriers, etc., to improve water-soluble swelling, increase tolerance, and reduce price cheap effect
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Embodiment 1
[0022] Embodiment 1 mixed fermentation culture collects Pseudomonas pseudoalcaligenes and Saccharomyces cerevisiae thalline, comprises the steps:
[0023] 1) Cultivation of Saccharomyces cerevisiae seed solution
[0024] Saccharomyces cerevisiae slant strains were inoculated on the primary seed medium for shake flask culture. After cultivating for 24 hours at 30°C with a rotation speed of 260rmp, they were inoculated in the secondary seed medium for fermentation tank cultivation at 30°C with a rotation speed of 260rmp and an aeration volume of 2.5 ~3m 3 After 24 hours of cultivation per hour, the seed liquid of Saccharomyces cerevisiae was obtained, wherein the primary seed medium was: yeast extract 5g / L, peptone 6g / L, glucose 20g / L; the secondary seed medium was: glucose 40g / L , yeast extract 5g / L, peptone 5g / L, (NH 4 ) 2 SO 42g / L, MgSO 4 ·7H 2 O0.25g / L, NaCl0.15g / L, KH 2 PO 4 2.8g / L.
[0025] 2) The cultivation of Pseudomonas alcaligenes seed solution
[0026] The ...
Embodiment 2
[0029] Embodiment 2 A preparation method of immobilized cell spheres, comprising the following steps:
[0030] A) After mixing polyvinyl alcohol, calcium alginate, acryloyl and water according to the mass ratio of 12:1:4:100, polyvinyl alcohol hydrosol is obtained, and the alkaligenic pseudomonas collected in Example 1 Bacteria and Saccharomyces cerevisiae cells were added to polyvinyl alcohol hydrosol to form a mixed solution, wherein the addition amount of Pseudomonas alcaligenes and Saccharomyces cerevisiae cells met the product of adding 5g wet weight per 100mL of polyvinyl alcohol hydrosol. Alkaline Pseudomonas and Saccharomyces cerevisiae mixed bacteria; B) Add the mixed solution to the solution containing 4w / v% N'-methylene bisacrylamide and 1.5w / v% CaSO with a peristaltic pump 4 In a saturated boric acid solution, gel balls are formed, placed at -20°C for 5-10 hours, thawed at room temperature, and then cooled at -10°C for 1-2 hours, after thawing at room temperature, ...
Embodiment 3
[0031] Embodiment 3 A preparation method of immobilized cell spheres, comprising the following steps:
[0032] A) After mixing polyvinyl alcohol, calcium alginate, acryloyl and water according to the mass ratio of 6:0.5:3:100, polyvinyl alcohol hydrosol is obtained, and the alkaligenic pseudomonas collected in Example 1 Bacteria and Saccharomyces cerevisiae cells were added to polyvinyl alcohol hydrosol to form a mixed solution, and the addition amount of Pseudomonas alcaligenes and Saccharomyces cerevisiae cells was controlled to meet the production of 4g wet weight per 100mL of polyvinyl alcohol hydrosol. Alkali Pseudomonas and Saccharomyces cerevisiae mixed bacteria; then the mixed solution was added to the solution containing 3w / v% N'-methylene bisacrylamide and 1w / v% CaSO with a peristaltic pump 4 In a saturated boric acid solution, gel balls are formed, placed at -20°C for 5-10 hours, thawed at room temperature, and then cooled at -10°C for 1-2 hours, after thawing at ro...
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