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Pseudomonas pseudoalcaligenes MOB13 and use thereof

An alkali-producing and pseudomonas-like technology, which is applied to the alkali-producing pseudomonas strain and its application in biological control of diseases and crop growth promotion, to achieve good disease prevention effect, promotion of wheat growth, and high environmental and economic benefits. Effect

Inactive Publication Date: 2009-06-24
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the application of Pseudomonas alcaligenes to the biological control of wheat sheath blight has not been reported

Method used

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  • Pseudomonas pseudoalcaligenes MOB13 and use thereof
  • Pseudomonas pseudoalcaligenes MOB13 and use thereof
  • Pseudomonas pseudoalcaligenes MOB13 and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1 Identification of MOB13

[0027] Based on the morphological characteristics, physiological and biochemical characteristics, 16SrDNA sequence and BIOLOG carbon source utilization results of Pseudomonas, it was identified as Pseudomonas pseudoalcaligenes.

[0028] The specific identification results are as follows:

[0029] 1. Morphological characteristics of bacteria

[0030] Gram staining was negative, electron microscope observation, bacteria oblong.

[0031] 2. Physiological and biochemical characteristics

[0032] The physiological and biochemical characteristics of Pseudomonas pseudoalcaligenes MOB13CGMCC NO.2763 are shown in Table 1: negative Gram staining, strictly aerobic, fructan, oxidase, arginine dihydrolysis, starch hydrolysis, gelatin hydrolysis , lecithinase, lipase, glucose oxidation to produce acid and gas, H 2 S production, anaphylactic necrosis, etc. were negative, while escin utilization, α-galactase reaction, catalase, and urease utiliza...

Embodiment 2

[0043] Example 2 Antibacterial Spectrum Analysis

[0044] Plate inhibition test method for pathogenic fungi. Use a hole punch to inoculate the target pathogenic fungus with a diameter of 5 mm on the center of the PDA plate. Pseudomonas and the target fungus are inoculated at 2.5 cm from both sides of the target pathogenic fungus at an angle of 180°. After culturing at 25°C for 4 to 6 days, measure the colony diameter of the pathogenic fungus . Plates inoculated with target fungi but not pseudomonas were used as controls. The growth inhibition rate was calculated according to the following formula:

[0045] Growth inhibition rate=(C-T) / C×100%

[0046] C: Control fungal growth diameter

[0047] T: diameter of fungal growth after inoculation with MOB13

[0048] Inhibition detection method for pathogenic bacteria plate, using double-layer culture method to detect the inhibition of pseudomonas to pathogenic bacteria. After activating MOB13 with PDA medium, make 10 with normal...

Embodiment 3

[0058] Example 3 Detection of biological control related traits

[0059] Protease can digest the membrane structure of pathogens, and can also destroy the body surface of nematodes, which is one of the biocontrol mechanisms of biocontrol bacteria. Detection of protease: detect protease with skimmed milk plate (tryptone 5g, yeast extract 2.5g, glucose 1g, 7% skimmed milk 250ml, agar 15g, supplemented with water 1000ml). Inoculate MOB13 into the center of the skimmed milk plate and incubate at 28°C for 72 hours. A transparent circle around the colony indicates protease positive. Such as Figure 4 Protease production by Pseudomonas pseudoalcaligenes MOB13 CGMCC NO.2763 is shown.

[0060] IAA is a phytohormone that can promote plant growth, and the IAA produced by bacteria has a growth-promoting effect on plants. Inoculate the activated MOB13 strain in NA medium (peptone 5g / L, yeast extract 1.5g / L, beef extract 1.5g / L, NaCl 5g / L, 0.5g / L tryptophan, pH7.0-7.2 ), after one week ...

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Abstract

The invention provides a pseudomonas pseudoalcaligenes new bacterial strain MOB13, the preserving number of the strain is CGMCC No.2763, and the new bacterial strain can be used for producing IAA and prolease which are related to antibacterium. The new bacterial strain has plate inhibiting effect on parts of plant pathogenic fungis (such as ceratocystis fimbriata, fusarium.oxysporum f.sp.niveum, monilinia laxa and phytophthora capsici). Plate, potted plant and field experiment are used for testing the control effect of Pseudomonad MOB13 to Rhizoctonia cerealis, and the control efficiency is respectively 62.13% and 73.87%. The new bacterial strain MOB13 has good growth promotion effect for wheat, can promote the plant height of wheat to increase 34.97%, and leads the root length to increase 17.34%.

Description

technical field [0001] The invention relates to a new microbial strain and its application, in particular to an alcaligenes-like pseudomonas strain and its application in biological disease control and crop growth promotion. Background technique [0002] Wheat sheath blight (Rhizoctonia cerealis), also known as blight blight and sharp eye spot, is one of the worldwide diseases with wide distribution. Almost all temperate wheat-growing regions of the world. Wheat sheath blight was reported abroad as early as 1934, and the disease was also discovered in my country in 1973, but it was not paid attention to because of its light onset and small area. Since the 1990s, with the continuous improvement of fertilization levels, sheath blight has occurred widely in the wheat areas of the middle and lower reaches of the Yangtze River in my country. The area of ​​wheat fields affected by sheath blight every year accounts for about one-fifth of the planting area, and the economic loss is...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/02A01P3/00A01P21/00C12R1/38
Inventor 吴文良邬娜郭岩彬孟凡乔乔玉辉
Owner CHINA AGRI UNIV
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