35results about How to "Strong toxic effect" patented technology

The invention relates to a degradable seedling culture container, and a preparation method and application thereof. The seedling culture container comprises preparation raw materials consisting of adhesive, a biomass powder material and a cross-linking agent and is characterized in that the adhesive is unsalted modified oxidized starch adhesive formed through formulation by taking plant starch asa base material, taking hydrogen peroxide as an oxidizer and taking an urea resin prepolymer and polyvinyl alcohol as modifiers; the plant starch is wheat starch or corn starch or potato starch; the biomass powder material is rice hull powder or straw powder or saw dust powder or other agricultural and forestry waste powder; and the cross-linking agent is low molecular polyamide. The preparation method of the seedling culture container comprises the following steps of: after oxidizing the plant starch through the oxidizer, modifying the plant starch through the modifiers to form the adhesive;and adding the adhesive and the cross-linking agent into the biomass powder material, and obtaining the degradable seedling culture container after stirring, dehydrating, moulding and forming. The degradable seedling culture container disclosed by the invention can be synchronously transplanted with seedlings after a seedling breeding period.

The invention relates to a serratia marcescens strain separated from phyllotreta striolata fabricius and applications thereof. A serratia marcescens strain is directly separated from phyllotreta striolata fabricius larva for the first time, and was preserved in China General Microbiological Culture Collection Center (CGMCC) in July 22th, 2013, with the preservation number of CGMCC No.7948. The serratia marcescens PS-1 strain has strong pathogenicity on the phyllotreta striolata fabricius larva and imagoes and laphygma exigua larva and imagoes, and can effectively control the development of laphygma exigua experiment populations. The serratia marcescens strain can provide technological base for the control on the field populations of phyllotreta striolata fabricius and laphygma exigua, and has important practical significance and practical values on safely, effectively and sustainably controlling the phyllotreta striolata fabricius and laphygma exigua and on efficiently ensuring the output and quality of vegetables.

The invention relates to activated bacillus thuringiensis HLJ-66 with Bt diamond back moth resistance and application thereof. The strain disclosed by the invention is bacillus thuringiensis HLJ-66, is called HLJ-66 for short and has the preservation number of CGMCCNo.8445, the preservation data of Nov. 8th in 2013 and the preservation address of No. 3, courtyard No.1, Beichen West Road, Chaoyang District of Beijing. The strain has toxicity for anti-Cry1Ac insecticidal crystal protein and Btk diamond back moth by toxicity biological assay screening. The strain is utilized to produce a high-efficiency broad-spectrum pesticide for preventing and treating the lepidopterous important pest of diamond back moth; and meanwhile, the strain has a good effect on resistance pests, is beneficial for preventing and treating resistance diamond back moth in the field, improves application value, is harmless for people, livestock and other animals, has no pollution to the environment and has excellent ecological benefits and excellent application and popularization prospect.

The invention discloses a planting method for promoting high yield of muskmelon, comprising: preparing a land and making a bed, subsoiling the land within a greenhouse, spraying Chinese herbal liquid, applying organic farm manure, and manufacturing nutrient pots; accelerating germination and sowing: sowing the sterilized muskmelon seeds in the nutrient pots according to a ratio of 1:1, sowing Hami seeds in the nutrient pots in which the muskmelon seeds are sowed according to a ratio of 1:1 after 5-7 days, and grafting when muskmelon seedlings grow to 6-7 cm in height and Hami seedlings grow to 4-5 cm in height; grafting and managing the grafting; carrying out field planting; carrying bioelectrical stimulating: using a bioelectrical generator to carry out bioelectrical stimulating 3-5 cm above the roots of grafted seedlings when the grafted seedlings start to extend, repeating once every 8-10 days until the muskmelons are mature and collected; managing fertilization; cooling and ventilating the greenhouse, and controlling disease and pest damage. The planting method can provide increased success rate of muskmelon grafted seedlings, increased yield of muskmelon, improved quality of muskmelon, and significantly improved economic benefit from muskmelon planting.

The invention relates to a novel gene of virus original protease inhibitor as well as the expression and the application thereof. The invention discloses a novel virus original protease inhibitor DNA sequence and an encoding protein sequence thereof, relates to a virus insect resistance key gene and enzyme and belongs to the field of gene engineering and protein engineering. The gene DNA sequence and the encoding amino acid sequence thereof are the first report of xenorhabdus nematophila. The protein expressed by the gene has remarkable inhibitory effect to prolease activity. A biological activity test shows that the protein encoded by the gene can reduce the reproduction rate of green peach aphids, improves the death of the green peach aphides and has good insecticide resistance function. The invention provides a new novel for the research of biological pesticides and transgenic zoophobous and has favorable application prospect in the field of pest control. The gene and the encoding protein thereof can also be applied in the field of medicament and biochemistry.

The invention discloses an environment-friendly halogen-free flame-retardant cable material, which comprises the following raw materials in parts by weight: 90-100 parts of LDPE resin, 30-50 parts of EPDM resin, 20-30 parts of EVA resin, 5-10 parts of PVC resin, hydrogen 100-120 parts of alumina, 20-24 parts of red phosphorus, 2-6 parts of anti-termite agent, 2-4 parts of stabilizer. The invention has excellent extrusion process performance, high LOI value, good flame retardancy, environmental protection and halogen-free.

A granular composite pesticide for cane features that the chlorpyrifos and phoxim are used as its main components and it contains also adsorbent, adhesive, coloring agent, synergist, stabilizer and carrier. Its advantages are high effect and durability, and high safety.

The invention discloses a highly active Bacillus thuringiensis HNCS-93 and its application, the preservation number is: CGMCC No. 8446, and the bacterium carries insecticidal protein coding genes cry1Ac and cry2Aa. The strain uses relatively sensitive Plutella xylostella, Btk-resistant Plutella xylostella, and Cry1Ac-resistant Plutella xylostella as test insects, and the virulent strains against Bt and Cry1Ac toxic protein Plutella xylostella are screened through virulence bioassays. The strain is used to produce high-efficiency and broad-spectrum insecticides, which are used to prevent and control the important lepidopteran pest Plutella xylostella. At the same time, it has a good effect on resistant pests, which is beneficial to the control of resistant Plutella xylostella in the field, and improves the economic value. It is harmless to humans, livestock and other animals, does not pollute the environment, has good economic and ecological benefits, and has good application and promotion prospects.

The invention discloses a Serratia marcescens PS-1 strain isolated from Flea beetle flavus and application thereof. The present invention directly isolates a strain of Serratia marcescens (Serratia marcescens) strain from the body of the diseased flea beetle larva for the first time, which was preserved in the General Microbiology Center of China Microbiology Culture Collection Management Committee on July 22, 2013. The number is CGMCC No.7948. The Serratia marcescens PS-1 strain has strong pathogenicity to the larvae and adults of the flavonoid beetle and the larvae and adults of the beet armyworm, and can effectively control the development of the experimental population of the beet armyworm. The invention provides a technical basis for the field population control of the flea beetle and the beet armyworm, and is important for safely, effectively and sustainably controlling the flea beetle and the beet armyworm, and effectively ensuring the yield and quality of vegetables practical significance and practical value.

The invention relates to activated bacillus thuringiensis HLJ-66 with Bt diamond back moth resistance and application thereof. The strain disclosed by the invention is bacillus thuringiensis HLJ-66, is called HLJ-66 for short and has the preservation number of CGMCCNo.8445, the preservation data of Nov. 8th in 2013 and the preservation address of No. 3, courtyard No.1, Beichen West Road, Chaoyang District of Beijing. The strain has toxicity for anti-Cry1Ac insecticidal crystal protein and Btk diamond back moth by toxicity biological assay screening. The strain is utilized to produce a high-efficiency broad-spectrum pesticide for preventing and treating the lepidopterous important pest of diamond back moth; and meanwhile, the strain has a good effect on resistance pests, is beneficial for preventing and treating resistance diamond back moth in the field, improves application value, is harmless for people, livestock and other animals, has no pollution to the environment and has excellent ecological benefits and excellent application and popularization prospect.

The invention relates to a novel gene of virus original protease inhibitor as well as the expression and the application thereof. The invention discloses a novel virus original protease inhibitor DNA sequence and an encoding protein sequence thereof, relates to a virus insect resistance key gene and enzyme and belongs to the field of gene engineering and protein engineering. The gene DNA sequenceand the encoding amino acid sequence thereof are the first report of xenorhabdus nematophila. The protein expressed by the gene has remarkable inhibitory effect to prolease activity. A biological activity test shows that the protein encoded by the gene can reduce the reproduction rate of green peach aphids, improves the death of the green peach aphides and has good insecticide resistance function.The invention provides a new novel for the research of biological pesticides and transgenic zoophobous and has favorable application prospect in the field of pest control. The gene and the encoding protein thereof can also be applied in the field of medicament and biochemistry.

The invention relates to recombined Bt genes mvip3Aa11, mcry2Ab4, an assortment of genes and application thereof, belonging to the technical field of biological prevention and control. The recombined genes mcry2Ab4 encode a protein having an amino acid sequence of SEQ NO 4 and has the nucleotide sequence of SEQ NO 3. The recombined genes mvip3Aa11 encode the protein with amino acid sequence of SEQ NO 2 and has the nucleotide sequence of SEQ NO 1. In the invention, the nucleotide compositions of the genes cry2Ab4 and vip3Aa11 are adjusted, so that the genes approach to the frequency of the codon of the plant gene and the encoded amino acid sequences are not changed; the assortments of recombined genes are expressed in the plant to obtain the resistance to the Lepidoptera. More importantly, the recombination of two proteins shows the remarkable cooperating effect and has very strong poisoning activity to resistant insects.