45results about How to "Overcoming or delaying drug resistance" patented technology

The invention discloses a bactericide pesticide composition containing chitosan oligosaccharide. The bactericide pesticide composition comprises a component A and a component B, wherein the component A is chitosan oligosaccharide or oligosaccharide derivatives; and the component B is one or more of active compounds. The bactericide pesticide composition has a synergistic effect, overcomes or delays pesticide resistance of bactericides, improves stress resistance and yield of crops, adjusts the metabolic balance in plants, improves the quality of agricultural products and enhances the application security of bactericides, and is safer for an ecological environment and people; and the using amount of chemical synthetic bactericides is reduced.

The invention discloses a pesticidal compound taking chlorantraniliprole and monosultap as active ingredients. The weight ratio of the chlorantraniliprole and the monosultap is 1:1 to 100, and the sum of the weight of the chlorantraniliprole and the monosultap is 10 to 50 percent of the total weight of the compound. The pesticidal compound is added with a solvent or a hydrotropy agent, an emulsifier, a dispersant, a sharing emulsifying agent, an antifreeze, a foam killer, a thickening agent, a stabilizing agent, an adhesive, etc. which are all different, and can be processed into different agent types such as an emulsion in water (EW), a microemulsion (ME), a suspending agent (SC), a wettable powder (WP), or a water dispersible grain agent (WDG), etc. The pesticidal compound can effectively prevent agricultural insects, in particular is applicable to prevent rice insect pests, has obviously synergized action for the poison function of insects, shows the characteristics of wide range of desinsection, high efficiency, rapid functional speed, low toxicity for mammals and natural enemy insects, low dosage and agricultural use cost, no cross resistance among medicaments, etc., and overcomes and defers the generation of the drug fastness of pests.

The invention relates to a bacillus thuringiensis insecticidal gene cry79Aa1, an expression protein and application thereof and belongs to the technical field of biological control. The insecticidal protein Cry79Aa separated from bacillus thuringiensis LTS290 (preseravtion number: CGMCC No.10232) has an amino acid sequence shown as SEQ ID NO.2, and a gene encoding the insecticidal protein, preferably the nucleotide sequence of the gene is shown as SEQ ID NO.1. The gene has certain toxicity to lepidopteran pests, so that the gene can be applied to transformation of microobes and plants, can show toxicity to related pests, and can overcome and delay the generation of drug resistance of the pests to engineering bacteria and transgenic plants.

The invention relates to a thuringiensis cry8I gene, protein and application thereof, which belong to the field of biotechnology, wherein the thuringiensis cry8I gene and the protein have high efficiency on coleopteran pests. The thuringiensis cry8I gene which is efficient on the coleopteran pests is obtained through the separation from a wild BT-SU4 strain, a sequence of the thuringiensis cry8I gene is shown in SEQ ID NO1, and an amino acid sequence of a coding of the thuringiensis cry8I gene is shown in SEQ ID NO2. A gene expression product has higher virulence to the coleopteran pests, and can overcome the disadvantages that Bt preparation products and gene species which are used for preventing the coleopteran pests in the world at present are single, make the pests easy to generate the resistance, have narrower insecticidal spectrum and lower virulence and so on; and the new gene can be applied to transforming microorganisms and plants, make the microorganisms and the plants show high virulence to related pests, and overcome and delay the occurrence of the drug resistance of the pests to engineering strains and transgenic plants.

The invention discloses a difenoconazole and biological antifungal substance HSAF (heat stable antifungal factor) containing fungicidal composition and application thereof. The fungicidal compositiontakes difenoconazole and HSAF as main effective components, and a mass ratio of difenoconazole to HSAF is 1:25-25:1. The fungicidal composition has synergistic effects in the above ratio range, drug resistance of germs is overcome and retarded, and high fungicidal speed, great prevention effects, long efficacy lasting period and low application cost are realized. The fungicidal composition is capable of preventing and controlling various crop fungal and oomycetes diseases and evidently superior to single agents in prevention and control effects on fungal diseases such as black spot, rot, scab,anthracnose, rust disease and the like of fruit trees and vegetables.

The invention relates to a bacillus thuringiensi strain, an expression protein and application of the protein, and belongs to the technical field of biological prevention control. Insecticide protein has an amino acid sequence shown in SEQ ID NO: 1 and a gene for encoding the insecticide protein, preferably the nucleotide sequence of the gene is as shown in SEQ ID NO: 2. The gene has high toxicity to rice planthopper injurious pests, can be applied to transformation of the microorganism and the plant to enable the microorganism and the plant to show the toxicity to relative injurious pests, and prevents and slows down the insecticide resistance generation of the injurious pests to engineering bacteria and transgenic plants.

The invention discloses a bactericidal composition for controlling main fungal diseases of pears and application thereof. The bactericidal composition adopts flusilazole, pyraclostrobin and a chitosanquaternary ammonium salt as the main components, according to different action mechanisms of flusilazole and pyraclostrobin, combines the induced disease resistance and the film-forming properties ofchitosan quaternary ammonium salt, and is assisted by appropriate auxiliary assistants, wherein the chitosan quaternary ammonium salt accounts for 50% of the mass of the main components, and the massratio of flusilazole to pyraclostrobin is 1:10-10:1. The bactericidal composition shows a synergistic effect in the above proportion range, has the characteristics of high efficiency, low toxicity, broad spectrum, long lasting period and low application cost, meanwhile delays the drug resistance of pathogenic bacteria, and reduces the dosage of chemical fungicides. The bactericidal composition can be used for controlling main fungal diseases of pears, especially pear black spot, pear scab, pear anthracnose, pear rust, pear fruit ring rot and other fungal diseases, and has obviously superior control effects to corresponding single agents and conventional compounding agents.

The invention belongs to the field of pesticides, in particular to a pesticide composition containing kasugamycin and polyoxamycin, the weight ratio of kasugamycin and polyoxamycin is 1:25-25:1, which can be used for the prevention and treatment of crop diseases , especially for the control of tomato leaf mold and tomato gray mold, with obvious synergistic effect.

The invention relates to "Bacillus thuringiensis LTS290, insecticidal gene cry57Ab, expressed protein and application thereof", belonging to the technical field of biological control. Bacillus thuringiensis LTS290, its preservation number is: CGMCC No.10232. The insecticidal protein isolated from strain LTS290 has the amino acid sequence shown in SEQ ID NO 2 and the gene encoding the insecticidal protein, preferably the nucleotide sequence of the gene is shown in SEQ ID NO1. The above-mentioned genes have certain toxicity to lepidopteran pests, and can be applied to transform microorganisms and plants to make them exhibit toxicity to related pests, and overcome and delay the generation of drug resistance of pests to engineering bacteria and transgenic plants.

The invention relates to a Bacillus thuringiensis insecticidal gene cry79Aa1, an expression protein and an application thereof, and belongs to the technical field of biological control. The insecticidal protein Cry79Aa isolated from Bacillus thuringiensis LTS290 (the deposit number: CGMCC No. 10232) has the amino acid sequence shown in SEQ ID NO. 2, and the gene encoding the insecticidal protein, preferably the gene The nucleotide sequence is shown in SEQ ID NO.1. The above-mentioned genes have certain virulence to lepidopteran pests, and can be applied to transform microorganisms and plants to show toxicity to related pests, and to overcome and delay the generation of drug resistance of pests to engineering bacteria and transgenic plants.

The invention relates to a bacillus thuringiensi strain, a combination protein and application of the protein, and belongs to the technical field of biological prevention control. The preservation number of the bacillus thuringiensi strain IPPBI0TSUC1O12 is CGMCC No. 10783, wherein proteins ST1 and ST2 extracted from the strain have high toxicity to rice planthopper injurious pests, can be applied to transformation of the microorganism and the plant to enable the microorganism and the plant to show the toxicity to relative injurious pests, and prevent and slow down the insecticide resistance generation of the injurious pests to engineering bacteria and transgenic plants.

The invention disclose an insecticidal composition using cyanogen insect amide and molosultap as effective components, prepared into aqueous emulsion (EW), micro emulsion (ME), suspending agent (SC), wettable powder (WP) or water dispersivity grain (WDG) by adding solvents and latent solvents, emulsifying agents, dispersing agents, antifreezing agents, defoaming agents, thickening agents, stabilizing agents or adhesives according to the method known by technicians in the technical field. The weight ratio of the cyanogen insect amide and the molosultap is 1:0.1-200, and the sum of the weights of the cyanogen insect amide and the molosultap is 2.2-70% of the total weight of the insecticidal composition. The composition has the remarkable synergized effect on the veneficium of insects, has the characteristics of broad insecticidal spectrum, high efficiency, quick effect, low poison to mammals and natural enemies, less insecticide amount, low agricultural cost, no cross resistance betweeninsecticides and the like, and overcomes and defers the generation of the insecticide resistance of injurious insects. The composition effectively prevents and controls agricultural injurious insectsand is particularly suitable for preventing and controlling rice injurious insects.

The invention discloses a difenoconazole and biological antifungal substance HSAF (heat stable antifungal factor) containing fungicidal composition and application thereof. The fungicidal compositiontakes difenoconazole and HSAF as main effective components, and a mass ratio of difenoconazole to HSAF is 1:25-25:1. The fungicidal composition has synergistic effects in the above ratio range, drug resistance of germs is overcome and retarded, and high fungicidal speed, great prevention effects, long efficacy lasting period and low application cost are realized. The fungicidal composition is capable of preventing and controlling various crop fungal and oomycetes diseases and evidently superior to single agents in prevention and control effects on fungal diseases such as black spot, rot, scab,anthracnose, rust disease and the like of fruit trees and vegetables.

The invention discloses an efficient insectofungicide containing beauveria bassiana and pyraclostrobin. Active ingredients of the product are beauveria bassiana and pyraclostrobin. Mass content of beauveria bassiana is 10-30%, and mass content of pyraclostrobin is 10-25%. The dosage form of the insectofungicide is an oil-suspended injection, wettable powder or water dispersible granules. The insectofungicide can be used for controlling plant diseases and insect pests for crops, and has an effect of treating plant diseases and insect pests for various crops, including main vegetable pests whitefly and diamondback moth and important vegetable diseases powdery mildew, downy mildew, late blight, early blight, anthracnose, etc. What's more important is that pyraclostrobin can enhance virulence of beauveria bassiana to diamondback moth.

The invention relates to "bacillus thuringiensis FH21, insecticidal gene, expressed protein and application thereof", belonging to the technical field of biological control. A strain of Bacillus thuringiensis FH21, the preservation number of which is CGMCC No.10090, four insecticidal proteins were isolated from the strain, having amino acid sequences as shown in SEQ ID NO 2, 4, 6, and 8, and encoding the insecticidal protein The gene of insect protein, preferably its nucleotide sequence is shown in SEQ ID NO1, 3, 5, 7. The bacterial strain and the above-mentioned genes have high toxicity to lepidopteran pests, and can be used to transform microorganisms and plants, so that they can show toxicity to related pests, and overcome and delay the development of pesticide resistance of pests to engineering bacteria and transgenic plants.

The invention discloses a sterilizing composition comprising prochloraz and biological antibacterial substance HSAF (heat stable antifungal factor) and application of the sterilizing composition. Theprochloraz and the HSAF are main effective components of the sterilizing composition. The mass ratio of the prochloraz to the HSAF ranges from 1:10 to 10:1. The sterilizing composition has synergisticinteraction effect within the matching range, overcomes and delays drug resistance of bacteria, and has high sterilization speed, good prevention and treatment effect, long lasting period and low application cost. The sterilizing composition can be used for preventing and controlling various crop fungus and oomycete diseases, especially fungus diseases such as black spots, anthracnose, scabs andrust diseases of pear trees and apple trees, and has obviously better prevention and control effect than a single agent.

The present invention relates to an insecticidal protein, its nucleotide sequence and its application. From the B4F11 strain (preservation number CGMCC NO.18611) isolated from the soil samples of Beijing Baiwangshan Forest Park, our laboratory obtained a positive clone of 1 gene. After purification and detection, the protein coded The gene has a total of 380 amino acids, as shown in SEQ ID NO: 1, and the coding gene has a total of 1143 bases, and the gene sequence is shown in SEQ ID NO: 2. The cloned gene was expressed, and the protein function verification showed that the protein had a strong poisonous effect on three kinds of rice planthoppers, especially on SBPH. The protein concentration is 30ppm, and the corrected mortality rate of the three rice planthoppers all reaches 90%, which has a good application prospect for the control of rice planthopper pests.