45results about How to "Overcoming or delaying drug resistance" patented technology

The invention discloses a bactericide pesticide composite containing oligochitosan, which comprises a component A and a component B, wherein the component A is oligochitosan or a derivative of the oligochitosan; and the component B is one or several of active compounds. The pesticide composition can produce a synergistic action, reduce the dosage of a chemically synthetic bactericide, prevent or delay the medicament resistance of the bactericide, improve crop stress resistance and yield, regulate metabolic balance in plant bodies and improve quality of agricultural products, improve the application safety of the bactericide and is more safe to ecological environmental and human bodies.

The invention discloses a bactericide pesticide composition containing chitosan oligosaccharide. The bactericide pesticide composition comprises a component A and a component B, wherein the component A is chitosan oligosaccharide or oligosaccharide derivatives; and the component B is one or more of active compounds. The bactericide pesticide composition has a synergistic effect, overcomes or delays pesticide resistance of bactericides, improves stress resistance and yield of crops, adjusts the metabolic balance in plants, improves the quality of agricultural products and enhances the application security of bactericides, and is safer for an ecological environment and people; and the using amount of chemical synthetic bactericides is reduced.

The invention relates to an insecticidal composition containing fluorin inspect bisamide and monosultap. The insecticidal composition has strong insecticidal activity, is applied to the field of chemical protection of agricultural production plants, is mainly used for preventing and curing the harm of lepidoptera pests to crops such as paddy rice, vegetables, fruit trees, cotton, seeding plants, flowers, and the like, has excellent insecticidal effect, and is different from other traditional insecticidal compositions. The insecticidal composition is prepared by combining effective ingredientsof the fluorin inspect bisamide and the monosultap. The insecticidal composition comprises the following preparation raw materials by weight percent: 0.1% to 50% of the fluorin inspect bisamide, 5% to 90% of the monosultap and 8% to 90% of additive. The composition preparation is aqueous emulsion, microemulsion, wettable powder, suspending agent, water dispersible granule or water soluble granule. The additive comprises solvent and one of or the mixture of latent solvent, emulsifying agent, dispersant, humectant, anti-freeze agent, thickening agent, defoaming agent, disintegrant, adhesive andauxiliary carrier.

The invention discloses a pesticidal compound taking chlorantraniliprole and monosultap as active ingredients. The weight ratio of the chlorantraniliprole and the monosultap is 1:1 to 100, and the sum of the weight of the chlorantraniliprole and the monosultap is 10 to 50 percent of the total weight of the compound. The pesticidal compound is added with a solvent or a hydrotropy agent, an emulsifier, a dispersant, a sharing emulsifying agent, an antifreeze, a foam killer, a thickening agent, a stabilizing agent, an adhesive, etc. which are all different, and can be processed into different agent types such as an emulsion in water (EW), a microemulsion (ME), a suspending agent (SC), a wettable powder (WP), or a water dispersible grain agent (WDG), etc. The pesticidal compound can effectively prevent agricultural insects, in particular is applicable to prevent rice insect pests, has obviously synergized action for the poison function of insects, shows the characteristics of wide range of desinsection, high efficiency, rapid functional speed, low toxicity for mammals and natural enemy insects, low dosage and agricultural use cost, no cross resistance among medicaments, etc., and overcomes and defers the generation of the drug fastness of pests.

The invention relates to recombined Bt genes mvip3Aa11, mcry2Ab4, an assortment of genes and application thereof, belonging to the technical field of biological prevention and control. The recombined genes mcry2Ab4 encode a protein having an amino acid sequence of SEQ NO 4 and has the nucleotide sequence of SEQ NO 3. The recombined genes mvip3Aa11 encode the protein with amino acid sequence of SEQ NO 2 and has the nucleotide sequence of SEQ NO 1. In the invention, the nucleotide compositions of the genes cry2Ab4 and vip3Aa11 are adjusted, so that the genes approach to the frequency of the codon of the plant gene and the encoded amino acid sequences are not changed; the assortments of recombined genes are expressed in the plant to obtain the resistance to the Lepidoptera. More importantly, the recombination of two proteins shows the remarkable cooperating effect and has very strong poisoning activity to resistant insects.

The invention relates to a bacillus thuringiensis cry1Ca gene, expressed protein and application of the bacillus thuringiensis cry1Ca gene and belongs to the technical field of biological prevention and control. Insecticidal protein has amino acid sequence shown as SEQ ID NO.2. In a gene for encoding the insecticidal protein, preferably, the nucleotide sequence of the gene is shown as SEQ ID NO.1. The gene has high toxicity on lepidoptera pests and is applied to transformation of microorganisms and plants, so that the microorganisms and the plants have toxicity on the related pests, and drug resistance of the pests on engineering bacteria and transgenic plants is overcome and delayed.

The invention relates to a bacillus thuringiensi strain, a combination protein and application of the protein, and belongs to the technical field of biological prevention control. The preservation number of the bacillus thuringiensi strain IPPBI0TSUC1O12 is CGMCC No. 10783, wherein proteins ST1 and ST2 extracted from the strain have high toxicity to rice planthopper injurious pests, can be applied to transformation of the microorganism and the plant to enable the microorganism and the plant to show the toxicity to relative injurious pests, and prevent and slow down the insecticide resistance generation of the injurious pests to engineering bacteria and transgenic plants.

The invention relates to a reconstructed mvip3Aa11 gene and application thereof, belonging to the technical field of biological prevention and control. The code of the reconstructed mvip3Aa11 gene has the protein of the amino acid sequence shown in SEQ NO2, and the nucleotide sequence is shown in SEQ NO1. In the invention, by adjusting the nucleotide composition of the vip2Aa11 gene, the vip2Aa11 gene is close to the codon frequency of plant gene without changing the coded amino acid sequence, and the reconstructed gene combination is expressed in plant so as to obtain resistance against lepidoptera pests.

The invention relates to bacillus thuringiensis LTS290 as well as an insecticidal gene cry57Ab, an expression protein and application of the bacillus thuringiensis LTS290, and belongs to the technical field of biological control. The preservation number of the bacillus thuringiensis LTS290 is CGMCC No.10232. The insecticidal protein separated from the bacterial strain LTS290 has the amino acid sequence as shown in SEQ ID NO.2 and a gene for encoding the insecticidal protein; the nucleotide sequence of the gene is preferred as SEQ ID NO.1. The gene has a certain killing force for lepidoptera pests, shows toxicity to related pests when being applied to the conversion of microorganisms and plants, and can be used for overcoming and delaying generation of drug resistance of pests to the engineering bacteria and transgenic plants.

The invention relates to a bacillus thuringiensis FH21, an insecticide gene, an expression protein and an application thereof, and belongs to the technical field of biological control. The collection number of the bacillus thuringiensis FH21strain is CGMCC No.10090, four insecticide proteins are separated out from the strain, the strain has the amino acid sequences represented by SEQ IN No 2, 4, 6, 8, and gene for encoding the insecticide proteins, preferably, the nucleotide sequences of the gene are represented by SEQ ID No 1, 3, 5, 7. The strain and the gene have high toxicity to lepidoptera pests, is applicable for converted microorganisms and plants to show the toxicity to related pests, and the resistance to drugs of the pests to engineering bacteria and transgenic plants are overcome and delayed.

The invention relates to a modified mcry2Ab4 gene and its application, and belongs to the technical field of biological control. The modified mcry2Ab4 gene encodes a protein with an amino acid sequence as shown in SEQ ID NO4. The nucleotide sequence is as shown in SEQ ID NO3. By regulating the nucleotide composition of cry2Ab4 gene, its codon frequency is similar to that of a plant gene without changing the encoding amino acid sequence, and the modified gene composition is expressed in plant so as to obtain the resistance to lepidoptera insects.

The invention discloses a fungicidal composition containing 2-methyl sulfone-5-(2,4-dichlorophenyl)-1,2,3-oxadiazole and fluopicolide and application of the fungicidal composition. The active ingredients comprise 2-methyl sulfone-5-(2,4-dichlorophenyl)-1,2,3-oxadiazole and fluopicolide, the compound weight ratio of 2-methyl sulfone-5-(2,4-dichlorophenyl)-1,2,3-oxadiazole to fluopicolide is 1:30-30:1, and preferred weight ratio is 1:20-10:1. The fungicidal composition can be prepared into emulsifiable concentrate, wettable powder, oil-dispersible suspending agent, suspending agent, water dispersible granule, aqueous emulsion, microemulsion and granules. The fungicidal composition is reasonable in composition and good in fungicidal effect, and can achieve an obvious synergistic effect within the proportion range. The fungicidal composition has remarkable control effects on potato late blight and other various crop diseases.

The invention relates to a bacillus thuringiensis insecticidal gene cry79Aa1, an expression protein and application thereof and belongs to the technical field of biological control. The insecticidal protein Cry79Aa separated from bacillus thuringiensis LTS290 (preseravtion number: CGMCC No.10232) has an amino acid sequence shown as SEQ ID NO.2, and a gene encoding the insecticidal protein, preferably the nucleotide sequence of the gene is shown as SEQ ID NO.1. The gene has certain toxicity to lepidopteran pests, so that the gene can be applied to transformation of microobes and plants, can show toxicity to related pests, and can overcome and delay the generation of drug resistance of the pests to engineering bacteria and transgenic plants.

The invention discloses a bactericide pesticide composition containing chitosan oligosaccharide. The bactericide pesticide composition comprises a component A and a component B, wherein the component A is chitosan oligosaccharide or oligosaccharide derivatives; and the component B is one or more of active compounds. The bactericide pesticide composition has a synergistic effect, overcomes or delays pesticide resistance of bactericides, improves stress resistance and yield of crops, adjusts the metabolic balance in plants, improves the quality of agricultural products and enhances the application security of bactericides, and is safer for an ecological environment and people; and the using amount of chemical synthetic bactericides is reduced.

The invention relates to a thuringiensis cry8I gene, protein and application thereof, which belong to the field of biotechnology, wherein the thuringiensis cry8I gene and the protein have high efficiency on coleopteran pests. The thuringiensis cry8I gene which is efficient on the coleopteran pests is obtained through the separation from a wild BT-SU4 strain, a sequence of the thuringiensis cry8I gene is shown in SEQ ID NO1, and an amino acid sequence of a coding of the thuringiensis cry8I gene is shown in SEQ ID NO2. A gene expression product has higher virulence to the coleopteran pests, and can overcome the disadvantages that Bt preparation products and gene species which are used for preventing the coleopteran pests in the world at present are single, make the pests easy to generate the resistance, have narrower insecticidal spectrum and lower virulence and so on; and the new gene can be applied to transforming microorganisms and plants, make the microorganisms and the plants show high virulence to related pests, and overcome and delay the occurrence of the drug resistance of the pests to engineering strains and transgenic plants.

The invention discloses a difenoconazole and biological antifungal substance HSAF (heat stable antifungal factor) containing fungicidal composition and application thereof. The fungicidal compositiontakes difenoconazole and HSAF as main effective components, and a mass ratio of difenoconazole to HSAF is 1:25-25:1. The fungicidal composition has synergistic effects in the above ratio range, drug resistance of germs is overcome and retarded, and high fungicidal speed, great prevention effects, long efficacy lasting period and low application cost are realized. The fungicidal composition is capable of preventing and controlling various crop fungal and oomycetes diseases and evidently superior to single agents in prevention and control effects on fungal diseases such as black spot, rot, scab,anthracnose, rust disease and the like of fruit trees and vegetables.

The invention relates to a bacillus thuringiensi strain, an expression protein and application of the protein, and belongs to the technical field of biological prevention control. Insecticide protein has an amino acid sequence shown in SEQ ID NO: 1 and a gene for encoding the insecticide protein, preferably the nucleotide sequence of the gene is as shown in SEQ ID NO: 2. The gene has high toxicity to rice planthopper injurious pests, can be applied to transformation of the microorganism and the plant to enable the microorganism and the plant to show the toxicity to relative injurious pests, and prevents and slows down the insecticide resistance generation of the injurious pests to engineering bacteria and transgenic plants.

The invention discloses an efficient insectofungicide containing beauveria bassiana and pyraclostrobin. Active ingredients of the product are beauveria bassiana and pyraclostrobin. Mass content of beauveria bassiana is 10-30%, and mass content of pyraclostrobin is 10-25%. The dosage form of the insectofungicide is an oil-suspended injection, wettable powder or water dispersible granules. The insectofungicide can be used for controlling plant diseases and insect pests for crops, and has an effect of treating plant diseases and insect pests for various crops, including main vegetable pests whitefly and diamondback moth and important vegetable diseases powdery mildew, downy mildew, late blight, early blight, anthracnose, etc. What's more important is that pyraclostrobin can enhance virulence of beauveria bassiana to diamondback moth.

The invention discloses a sterilizing composition comprising prochloraz and biological antibacterial substance HSAF (heat stable antifungal factor) and application of the sterilizing composition. Theprochloraz and the HSAF are main effective components of the sterilizing composition. The mass ratio of the prochloraz to the HSAF ranges from 1:10 to 10:1. The sterilizing composition has synergisticinteraction effect within the matching range, overcomes and delays drug resistance of bacteria, and has high sterilization speed, good prevention and treatment effect, long lasting period and low application cost. The sterilizing composition can be used for preventing and controlling various crop fungus and oomycete diseases, especially fungus diseases such as black spots, anthracnose, scabs andrust diseases of pear trees and apple trees, and has obviously better prevention and control effect than a single agent.

The invention discloses a bactericidal composition for controlling main fungal diseases of pears and application thereof. The bactericidal composition adopts flusilazole, pyraclostrobin and a chitosanquaternary ammonium salt as the main components, according to different action mechanisms of flusilazole and pyraclostrobin, combines the induced disease resistance and the film-forming properties ofchitosan quaternary ammonium salt, and is assisted by appropriate auxiliary assistants, wherein the chitosan quaternary ammonium salt accounts for 50% of the mass of the main components, and the massratio of flusilazole to pyraclostrobin is 1:10-10:1. The bactericidal composition shows a synergistic effect in the above proportion range, has the characteristics of high efficiency, low toxicity, broad spectrum, long lasting period and low application cost, meanwhile delays the drug resistance of pathogenic bacteria, and reduces the dosage of chemical fungicides. The bactericidal composition can be used for controlling main fungal diseases of pears, especially pear black spot, pear scab, pear anthracnose, pear rust, pear fruit ring rot and other fungal diseases, and has obviously superior control effects to corresponding single agents and conventional compounding agents.

The invention belongs to the field of pesticides, in particular to a pesticide composition containing kasugamycin and polyoxamycin, the weight ratio of kasugamycin and polyoxamycin is 1:25-25:1, which can be used for the prevention and treatment of crop diseases , especially for the control of tomato leaf mold and tomato gray mold, with obvious synergistic effect.

The invention relates to "Bacillus thuringiensis LTS290, insecticidal gene cry57Ab, expressed protein and application thereof", belonging to the technical field of biological control. Bacillus thuringiensis LTS290, its preservation number is: CGMCC No.10232. The insecticidal protein isolated from strain LTS290 has the amino acid sequence shown in SEQ ID NO 2 and the gene encoding the insecticidal protein, preferably the nucleotide sequence of the gene is shown in SEQ ID NO1. The above-mentioned genes have certain toxicity to lepidopteran pests, and can be applied to transform microorganisms and plants to make them exhibit toxicity to related pests, and overcome and delay the generation of drug resistance of pests to engineering bacteria and transgenic plants.

The invention relates to a Bacillus thuringiensis insecticidal gene cry79Aa1, an expression protein and an application thereof, and belongs to the technical field of biological control. The insecticidal protein Cry79Aa isolated from Bacillus thuringiensis LTS290 (the deposit number: CGMCC No. 10232) has the amino acid sequence shown in SEQ ID NO. 2, and the gene encoding the insecticidal protein, preferably the gene The nucleotide sequence is shown in SEQ ID NO.1. The above-mentioned genes have certain virulence to lepidopteran pests, and can be applied to transform microorganisms and plants to show toxicity to related pests, and to overcome and delay the generation of drug resistance of pests to engineering bacteria and transgenic plants.

The invention relates to a bacillus thuringiensi strain, a combination protein and application of the protein, and belongs to the technical field of biological prevention control. The preservation number of the bacillus thuringiensi strain IPPBI0TSUC1O12 is CGMCC No. 10783, wherein proteins ST1 and ST2 extracted from the strain have high toxicity to rice planthopper injurious pests, can be applied to transformation of the microorganism and the plant to enable the microorganism and the plant to show the toxicity to relative injurious pests, and prevent and slow down the insecticide resistance generation of the injurious pests to engineering bacteria and transgenic plants.

The invention disclose an insecticidal composition using cyanogen insect amide and molosultap as effective components, prepared into aqueous emulsion (EW), micro emulsion (ME), suspending agent (SC), wettable powder (WP) or water dispersivity grain (WDG) by adding solvents and latent solvents, emulsifying agents, dispersing agents, antifreezing agents, defoaming agents, thickening agents, stabilizing agents or adhesives according to the method known by technicians in the technical field. The weight ratio of the cyanogen insect amide and the molosultap is 1:0.1-200, and the sum of the weights of the cyanogen insect amide and the molosultap is 2.2-70% of the total weight of the insecticidal composition. The composition has the remarkable synergized effect on the veneficium of insects, has the characteristics of broad insecticidal spectrum, high efficiency, quick effect, low poison to mammals and natural enemies, less insecticide amount, low agricultural cost, no cross resistance betweeninsecticides and the like, and overcomes and defers the generation of the insecticide resistance of injurious insects. The composition effectively prevents and controls agricultural injurious insectsand is particularly suitable for preventing and controlling rice injurious insects.

The invention discloses a difenoconazole and biological antifungal substance HSAF (heat stable antifungal factor) containing fungicidal composition and application thereof. The fungicidal compositiontakes difenoconazole and HSAF as main effective components, and a mass ratio of difenoconazole to HSAF is 1:25-25:1. The fungicidal composition has synergistic effects in the above ratio range, drug resistance of germs is overcome and retarded, and high fungicidal speed, great prevention effects, long efficacy lasting period and low application cost are realized. The fungicidal composition is capable of preventing and controlling various crop fungal and oomycetes diseases and evidently superior to single agents in prevention and control effects on fungal diseases such as black spot, rot, scab,anthracnose, rust disease and the like of fruit trees and vegetables.

The invention discloses an efficient insectofungicide containing beauveria bassiana and pyraclostrobin. Active ingredients of the product are beauveria bassiana and pyraclostrobin. Mass content of beauveria bassiana is 10-30%, and mass content of pyraclostrobin is 10-25%. The dosage form of the insectofungicide is an oil-suspended injection, wettable powder or water dispersible granules. The insectofungicide can be used for controlling plant diseases and insect pests for crops, and has an effect of treating plant diseases and insect pests for various crops, including main vegetable pests whitefly and diamondback moth and important vegetable diseases powdery mildew, downy mildew, late blight, early blight, anthracnose, etc. What's more important is that pyraclostrobin can enhance virulence of beauveria bassiana to diamondback moth.

The invention relates to "bacillus thuringiensis FH21, insecticidal gene, expressed protein and application thereof", belonging to the technical field of biological control. A strain of Bacillus thuringiensis FH21, the preservation number of which is CGMCC No.10090, four insecticidal proteins were isolated from the strain, having amino acid sequences as shown in SEQ ID NO 2, 4, 6, and 8, and encoding the insecticidal protein The gene of insect protein, preferably its nucleotide sequence is shown in SEQ ID NO1, 3, 5, 7. The bacterial strain and the above-mentioned genes have high toxicity to lepidopteran pests, and can be used to transform microorganisms and plants, so that they can show toxicity to related pests, and overcome and delay the development of pesticide resistance of pests to engineering bacteria and transgenic plants.

The present invention relates to an insecticidal protein, its nucleotide sequence and its application. From the B4F11 strain (preservation number CGMCC NO.18611) isolated from the soil samples of Beijing Baiwangshan Forest Park, our laboratory obtained a positive clone of 1 gene. After purification and detection, the protein coded The gene has a total of 380 amino acids, as shown in SEQ ID NO: 1, and the coding gene has a total of 1143 bases, and the gene sequence is shown in SEQ ID NO: 2. The cloned gene was expressed, and the protein function verification showed that the protein had a strong poisonous effect on three kinds of rice planthoppers, especially on SBPH. The protein concentration is 30ppm, and the corrected mortality rate of the three rice planthoppers all reaches 90%, which has a good application prospect for the control of rice planthopper pests.