A kind of insecticidal protein, its nucleotide sequence and its application

Abstract

The present invention relates to an insecticidal protein, its nucleotide sequence and its application. From the B4F11 strain (preservation number CGMCC NO.18611) isolated from the soil samples of Beijing Baiwangshan Forest Park, our laboratory obtained a positive clone of 1 gene. After purification and detection, the protein coded The gene has a total of 380 amino acids, as shown in SEQ ID NO: 1, and the coding gene has a total of 1143 bases, and the gene sequence is shown in SEQ ID NO: 2. The cloned gene was expressed, and the protein function verification showed that the protein had a strong poisonous effect on three kinds of rice planthoppers, especially on SBPH. The protein concentration is 30ppm, and the corrected mortality rate of the three rice planthoppers all reaches 90%, which has a good application prospect for the control of rice planthopper pests.

Description

technical field [0001] The present invention relates to the technical field of biological control, in particular further, the present invention relates to an insecticidal gene with high virulence against rice planthopper agricultural pests and a protein encoded by the gene, and the use of the insecticidal protein in the control of rice planthopper Applications. Background technique [0002] Rice is one of the three major staple crops in my country, and its safe production is related to the national economy and people's livelihood. Rice planthopper is an important piercing-sucking pest on rice, which mainly includes three groups: Laodelphax striatellus, Nilaparvata lugens and Sogatella furcifera. Such pests belong to the Hemiptera planthopper family. They mainly suck the juice of rice and other crops through their piercing-sucking mouthparts, plundering their nutrients, resulting in an increase in the rate of empty seedlings, a decrease in the thousand-grain weight, and a de...

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Problems solved by technology

[0004] At present, the control of such pests mainly depends on the control of chemical pesticides, such as chlorpyrifos, imidacloprid, malathion, pymetrozine, etofenproxil, etc. However, the long-term abuse of chemical pesticides has brought about the increase of pest resistance, pesticide residues, environmental Pollution, food safety and other serious consequences, there is an urgent need to discover proteins with high insecticidal activity against such pests for biological control and transgenic plants

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