Complex microbial inoculant for black and odorous water body as well as preparation method and application thereof

A compound bacterial agent, black and odorous water technology, applied in biochemical equipment and methods, chemical instruments and methods, microorganism-based methods, etc., can solve the problems of unsatisfactory treatment effect, single microbial effect, difficult to widely use, etc. Achieve the effect of eliminating odor, improving water transparency, strong combination and synergy

Active Publication Date: 2019-04-05
合肥市东方美捷分子材料技术有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In recent years, microbial agents have been used in the treatment of black and odorous water bodies, but there are still many problems, such

Method used

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  • Complex microbial inoculant for black and odorous water body as well as preparation method and application thereof
  • Complex microbial inoculant for black and odorous water body as well as preparation method and application thereof
  • Complex microbial inoculant for black and odorous water body as well as preparation method and application thereof

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Experimental program
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Effect test

preparation example Construction

[0032]The preparation process of each bacterial strain bacterium liquid used in the embodiment of the present invention is as follows:

[0033] (1) Four strains of Pseudomonas slough, Pseudomonas alcaligenes, Paracoccus denitrificans and Micrococcus lyticus were picked and inoculated into solid slant medium for cultivation, the cultivation temperature was 28°C, and the cultivation time was After 24 hours, after the growth of colonies on the slant medium, inoculate it into the liquid medium for shaker culture, the shaker speed is 150-180r / min, the culture temperature is 28-30°C, and the culture time is 24-36h , when the number of live bacteria reaches 1×10 8 When the CFU / mL was reached, the preparation of the bacterial solution was completed, and the bacterial solutions of Pseudomonas saccharides, Pseudomonas alcaligenes, Paracoccus denitrificans and Micrococcus lysolyticus were obtained respectively.

[0034] The solid slant medium is prepared by mixing the following raw mate...

Embodiment 1

[0039] Mix the bacterial solutions of Pseudomonas sludge, Pseudomonas alcaligenes, Geobacillus stearothermophilus, Paracoccus denitrificans and Micrococcus lyticus at a volume ratio of 1:2:3:3:1, and then After high-temperature sterilization, inoculate with a volume ratio of 10% of the fermentation medium, ferment at 30°C for 5 days, and when the number of viable bacteria reaches 5×10 7 When the CFU / mL is above, through centrifugal filtration and freeze-drying at -21°C until the water content is less than or equal to 5%, the composite bacterial agent for treating black and odorous water can be obtained. The fermentation medium is made by mixing the following raw materials in weight ratio: brown sugar 15-25g, peptone 9-10g, beef extract 3-5g, NaCl 2-5g, KH 2 PO 4 0.75-1.5g, K 2 HPO 4 0.8-1g, supplemented with distilled water to 1L, pH = 7, sterilized at 121°C for 30min and cooled before use.

[0040] The composite bacterial agent is mainly used for the treatment of black a...

Embodiment 2

[0046] Others are the same as in Example 1, with the water body 2.5 / 10000 adding the composite bacterial agent of the present invention, taking water samples for testing every three days, and observing the changes in water quality. The experimental results show that after adding the composite bacterial agent of the present invention, the phenomenon of black odor is obviously weakened on the 6th day, the transparency of water quality is improved, and the peculiar smell of the water body is obviously weakened. After 18 days, COD, NH 3 The removal rates of -N and TP content reached 91.08%, 74.11% and 91.85%, respectively. For removal see figure 2 , the water quality indicators of each stage after treatment are shown in Table 2.

[0047] Table 2 Various indicators of water quality in different stages of the black and odorous river treatment

[0048] governance stage

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Abstract

The invention discloses a complex microbial inoculant for a black and odorous water body as well as a preparation method and application thereof. The complex microbial inoculant is obtained by singlyculturing strains such as pseudomonas borbori, pseudomonas alcalophila, geobacillus thermophilus, paracoccus denitrificans and micrococcus lysodeikticus, performing mixed inoculation and fermentation,and then performing collection. The metabolic function of the strains contained in the complex microbial inoculant has high synergetic complementarity; the thallus reproduction rate is high; the production cost is low; the environment protection is achieved; no pollution exits; the performance is excellent and efficient; the water quality of the black and odorous water body can be effectively improved; the COD, NH3-N, TP, and odor degree and chromaticity of the water body can be effectively reduced; the transparency of the water body is improved.

Description

technical field [0001] The invention relates to a composite bacterial agent for black and odorous water bodies, a preparation method and application thereof, and belongs to the technical field of sewage treatment. Background technique [0002] With the continuous acceleration of urbanization in my country, the continuous discharge of urban sewage has caused eutrophication of water bodies, and even led to black and smelly water bodies, seriously affecting people's living environment and life, and hindering social and economic development. The phenomenon of eutrophication and black odor in water bodies such as rivers and lakes is mainly due to the increase in the content of trophic pollutants in the water body, which leads to severe anoxic water bodies, and anaerobic microorganisms digest organic pollutants and produce a large amount of malodorous gases (such as hydrogen sulfide, Ammonia and mercaptans, etc.), causing the water body to smell; metal ions such as iron and mangan...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C12R1/38C12R1/07C12R1/265C12R1/01
CPCC12N1/20C02F3/34
Inventor 朱守诚桑建伟黄家榜杨宏星孙庆红黄浩程鹏飞周锐
Owner 合肥市东方美捷分子材料技术有限公司
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