Composite probiotic agent for improving egg quality of laying hens, preparation method and application
A compound probiotic and egg quality technology, applied in biochemical equipment and methods, bacteria used in food preparation, and microbial-based methods, to achieve the effects of improving egg production quality, intestinal function, and nutritional value
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Embodiment 1
[0019] This embodiment provides a compound probiotic agent for improving the quality of laying eggs, including compound probiotic bacteria and a carrier, and the preparation method of the compound probiotic agent comprises the following steps:
[0020] (1) After activating Bacillus Velez, plant lactic acid bacteria, Bacteroides faecalis and Pseudomonas alcaligenes respectively, they were inoculated in LB liquid medium for expanded culture;
[0021] (2) Bacillus Veles and Lactobacillus plantarum were expanded and cultured to a colony concentration of 4.0×10 10 CFU / mL, Bacteroides faecalis and Pseudomonas alcaligenes were expanded to a colony concentration of 1.0×10 8 CFU / mL, and then mix the four bacterial solutions in equal volumes to obtain a mixed bacterial solution of compound probiotics;
[0022] (3) Prepare the carrier: mix the rice bran powder and starch according to the mass ratio of 1:8;
[0023] (4) Add 1kg of carrier to every 100ml of compound probiotics, and mix w...
Embodiment 2
[0025] The present embodiment provides a kind of composite probiotic agent that improves the quality of laying eggs, including composite probiotic bacteria and carrier, and the preparation method of this composite probiotic agent comprises the following steps:
[0026] (1) Inoculate Bacillus Velez, Lactobacillus plantarum, Bacteroides faecalis and Pseudomonas alcaligenes in LB liquid medium for expanded culture respectively;
[0027] (2) Bacillus Veles and Lactobacillus plantarum were expanded and cultured to a colony concentration of 4.5×10 10 CFU / mL, Bacteroides faecalis and Pseudomonas alcaligenes were expanded to a colony concentration of 1.5×10 8 CFU / mL, and then mix the four bacterial solutions in equal volumes to obtain a mixed bacterial solution of compound probiotics;
[0028] (3) Prepare the carrier: mix the rice bran powder and starch according to the mass ratio of 1:10;
[0029] (4) Add 1.5kg of carrier to every 100ml of compound probiotics, and mix well to obtai...
Embodiment 3
[0031] The present embodiment provides a kind of composite probiotic agent that improves the quality of laying eggs, including composite probiotic bacteria and carrier, and the preparation method of this composite probiotic agent comprises the following steps:
[0032] (1) Inoculate Bacillus Velez, Lactobacillus plantarum, Bacteroides faecalis and Pseudomonas alcaligenes in LB liquid medium for expanded culture respectively;
[0033] (2) Expand the cultivation of Bacillus Velez and Lactobacillus plantarum to a colony concentration of 4.2×10 10 CFU / mL, Bacteroides faecalis and Pseudomonas alcaligenes were expanded to a colony concentration of 1.2×10 8 CFU / mL, and then mix the four bacterial solutions in equal volumes to obtain a mixed bacterial solution of compound probiotics;
[0034] (3) Prepare the carrier: mix the rice bran powder and starch according to the mass ratio of 1:9;
[0035] (4) Add 1.2kg of carrier to every 100ml of compound probiotics, and mix well to obtain ...
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