Complex microbial inoculant for petroleum hydrocarbon and benzene series treatment as well as preparation method and application thereof
A technology of composite bacterial agents and benzene series, which is applied in the fields of biotechnology and environmental protection, can solve the problems of limiting the scope of application of exogenous microbial bacterial agents, the use effect, and the slow degradation speed, so as to speed up the degradation speed, reduce the addition, cost-saving effect
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[0054] Four, the preparation of composite bacterial agent:
[0055] Mix the fermentation cultures of the components of the above obtained composite bacterial agent in proportion, and adjust the fermentation culture time and the mass percentage content of the solid fermentation culture to obtain a composite bacterial agent containing a specific effective number of viable bacteria.
[0056] In the composite microbial agent of the present invention, the mass percentage of the effective viable count of each strain accounting for the total effective viable bacterial count of the composite microbial agent is as follows:
[0057] Aspergillus oryzae 30-35%, Neurospora sp 30-35%, Pseudomonas putida 10-15%, Pseudomonas alcaligenes 10-15% %, Brevibacterium epidermidis 5-10%, Erwinia sp 5-10%.
[0058] The formula of above-mentioned each culture medium is as follows:
[0059] Strain preservation medium: beef extract 5g, peptone 10g, NaCl 5g, agar 20g, distilled water 1000mL, pH 7.0;
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Embodiment 1
[0067] The present embodiment provides a kind of (stabilization of urban stock garbage) composite bacterial agent, the total effective number of viable bacteria of the composite bacterial agent is 10 10 cfu / mL, the effective viable count of each strain accounts for the mass percent of the total effective viable count of the composite microbial agent as follows:
[0068] Aspergillus oryzae 35%, Neurospora 30%, Pseudomonas putida 10%, Pseudomonas alcaligenes 10%, Brevibacterium epidermidis 10%, Erwinia 5%.
Embodiment 2
[0070] The present embodiment provides a kind of composite bacterial agent, the total effective number of viable bacteria of the composite bacterial agent is 10 10 cfu / mL, the effective viable count of each strain accounts for the mass percent of the total effective viable count of the composite microbial agent as follows:
[0071] Aspergillus oryzae 30%, Neurospora 30%, Pseudomonas putida 10%, Pseudomonas alcaligenes 15%, Brevibacterium epidermidis 5%, Erwinia 10%.
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