Pseudomonas compound microbial agent and application thereof in disease resistance, yield increase and quality improvement of angelica sinensis

A technology of composite microbial agent and Pseudomonas, which is applied in the direction of microorganism-based methods, microorganisms, biocides, etc., can solve the problems of not being able to improve at the same time, and improving disease resistance without angelica growth, so as to achieve the goal of improving resistance Effect

Active Publication Date: 2021-09-17
GANSU ACAD OF SCI INST OF BIOLOGY +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

And existing literature reports, the content of plant endogenous salicylic acid and jasmonic acid is negatively correlated, that is, can not improve salicylic acid and jasmonic acid in Angelica sinensis simultaneously.
At present, there is still no microbial agent that can simultaneously promote the growth of Angelica sinensis, improve disease resistance, improve the appearance quality of Angelica sinensis, and the content of various iconic components

Method used

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  • Pseudomonas compound microbial agent and application thereof in disease resistance, yield increase and quality improvement of angelica sinensis
  • Pseudomonas compound microbial agent and application thereof in disease resistance, yield increase and quality improvement of angelica sinensis
  • Pseudomonas compound microbial agent and application thereof in disease resistance, yield increase and quality improvement of angelica sinensis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Example 1 Composite microbial bacterial agent related enzyme activity detection

[0038] Inoculate the above-mentioned bacteria into 20mL PDB medium respectively, shake culture (30°C, 180r / min) for 24 hours; collect the bacteria by centrifugation at 5000r / min, resuspend the bacteria with sterilized distilled water until the turbidity of the bacteria solution is about 1 , add 1mL of the corresponding enzyme extract in the kit to a 1.5mL centrifuge tube, and add 100μL of the bacteria solution to be tested, break up the bacteria by ultrasonication in an ice bath (power 200W), ultrasonication for 3s, with an interval of 10s, and repeat 30 times; the ultrasonicated bacteria Centrifuge the solution for 10 min (4°C, 9000r / min), draw the supernatant into a new centrifuge tube, and put it on ice for testing; measure the enzyme activity according to the method described in the corresponding assay kit, and divide the calculated results by the corresponding Turbidity of bacterial s...

Embodiment 2

[0042] Example 2 Effects of Different Inoculum Treatment on Physiological and Biochemical Characteristics of Angelica Sinensis Leaf

[0043] According to the method described in the kit, the chlorophyll, MDA, H 2 o 2 Content, SOD, POD, CAT, PAO, DAO, PPO activity. Each sample was repeated 3 times and the average value was calculated.

[0044] The result is as figure 1 Shown, compound microbial agent T1 of the present invention, positive control group Bacillus compound bacterial agent T2 all significantly improved the activity of CAT, SOD, POD, PAO, DAO, PPO in Angelica blade compared with negative control group CK, and H 2 o 2 , chlorophyll content, significantly reduced the malondialdehyde content (P<0.05) in the leaves, and both treatments have played a better stress protection effect; compared with the positive control group Bacillus composite bacterial agent T2, the present invention After the compound microbial agent T1 treatment, the activities of CAT, POD, PAO and...

Embodiment 3

[0045] The influence of embodiment 3 different treatments on Angelica sinensis endogenous hormone

[0046] According to the method reported in the literature "Effects of Nano-iron and Melatonin on the Yield and Quality of Gansu Fritillaria Under Domesticated Cultivation Conditions" (Chinese Journal of Experimental Formulas, 2021), UPLC-MS was used to determine the contents of different endogenous hormones in the leaves of Angelica sinensis, Each sample was repeated 3 times and the average value was calculated.

[0047] The result is as shown in table 2, except salicylic acid, auxin, gibberellin, composite microbial agent T1 of the present invention, positive control group Bacillus composite bacterial agent T2 are more than negative control group CK, other hormone two All showed a consistent trend of change, significantly increasing the content of jasmonic acid, which increased by 257.91% and 99.94% respectively; reducing the content of abscisic acid, which decreased by 64.69% ...

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Abstract

The invention belongs to the technical field of microorganisms and biology, and particularly relates to a compound microbial agent of microorganisms and application of the compound microbial agent in disease resistance, yield increase and quality improvement of angelica sinensis. The compound microbial agent is prepared by compounding pseudomonas fluorescens, pseudomonas alcaligenes and pseudomonas psychrophila; the compound microbial agent has ACC deaminase activity, SOD activity, POD activity, NEX neutral xylanase activity, chitinase activity and laccase activity; the growth of medicinal organ roots of the underground part of the angelica sinensis can be promoted, the rhizome diameter, rhizome length, main root length and average single plant weight are increased, the yield is increased, and the appearance character of the commercial angelica sinensis is improved; the content of iconic components such as ferulic acid, Z-ligustilide and senkyunolide A, endogenous jasmonic acid and salicylic acid of the angelica sinensis can be increased, the quality of the angelica sinensis can be remarkably improved, and the compound microbial agent can be used for preparing the special microbial fertilizer for the angelica sinensis; and the compound microbial agent can prevent and treat plant diseases caused by fusarium pathogenic fungi, and can be used for preparing pesticides for preventing and treating plant diseases.

Description

technical field [0001] The invention relates to the field of microorganisms and biotechnology, in particular to a compound bacterial agent of microorganisms and its application in increasing production and quality of Angelica sinensis against diseases. Background technique [0002] Academician Huang Luqi of the Chinese Academy of Chinese Medical Sciences and Guo Lanping, a researcher team, proposed the "ecological planting of Chinese herbal medicines" model, which not only pays attention to the ecological conditions of the site, but also pays more attention to the input of agricultural inputs. It is the main mode of Chinese herbal medicines production at present. Pay attention to waste recycling and species diversity, do not use chemically synthesized fertilizers, pesticides, and plant growth regulators, and promote the use of animal sources, plant sources, microbial sources, natural minerals and other products are its basic requirements. [0003] Plant growth-promoting rhiz...

Claims

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Application Information

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IPC IPC(8): C12N1/20C05F11/08C05G3/00C05G3/60A01N63/27A01P3/00A01P21/00C12R1/39C12R1/38
CPCC12N1/20C05F11/08C05G3/00C05G3/60A01N63/27
Inventor 杨涛李有忠王治业杨晖王引权姚阳阳赵疆李鑫
Owner GANSU ACAD OF SCI INST OF BIOLOGY
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