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Method for preparing chitin/chitosan from rind and shell of silkworm chrysalis and fly maggot

A chitin and chitosan technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of low quality of chitin products, difficulty in preparing chitin, and excessive fat in the skin , to achieve beneficial quality control, excellent stability, and no pollution

Active Publication Date: 2007-11-28
CHONGQING LIKE BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, although silkworm chrysalis and fly maggots have the above-mentioned advantages, recent practice has shown that there are difficulties in preparing chitin from silkworm chrysalis and fly maggots by chemical methods. The base is difficult, so the quality of chitin products produced is not high
In addition, due to the addition of chemical degreasing steps in the extraction process, the physical / chemical treatment of decolorization and deacetylation is strengthened, resulting in greater waste liquid, waste pollution and more energy consumption

Method used

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  • Method for preparing chitin/chitosan from rind and shell of silkworm chrysalis and fly maggot
  • Method for preparing chitin/chitosan from rind and shell of silkworm chrysalis and fly maggot
  • Method for preparing chitin/chitosan from rind and shell of silkworm chrysalis and fly maggot

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0060] Embodiment 1: the preparation of silkworm chrysalis chitin

[0061] a. Pretreatment: Collect 10kg of fresh silkworm chrysalis, wash and remove impurities, fully drain, and then use a meat grinder to mechanically break the skin, squeeze out the contents with a pressing method (hydraulic or screw press) (to be processed separately), and collect the skin The shells are dried until the water content is less than 10%, and pre-frozen at below 0°C for 6 hours. After agglomeration, the shells are crushed to a fine powder with an average particle size of 150-250 μm (60-100 mesh) with a centrifugal impact crusher.

[0062] b. The obtained fine powder is first soaked in hot water at 65°C for 30 minutes, poured out, and then mixed with clean warm water at 40°C and an equal volume of lipase and protease at a ratio of solid:liquid=1:4. Enzyme solution, at a constant temperature of 40°C, slowly mix the leather shell fine powder and crude enzyme solution in a reaction kettle, and carry...

Embodiment 2

[0065] Embodiment 2: the preparation of fly maggot chitosan

[0066] a. Pretreatment: collect 10kg of fresh fly maggots, wash and remove impurities, fully drain, and then use a meat grinder to mechanically break the skin, squeeze out the contents with a pressing method (hydraulic or screw press) (to be processed separately), and collect the skin The shells are dried until the moisture content is less than 10%, pre-frozen at below 0°C for 6 hours, and after hardening, crush them with a centrifugal impact mill to a fine powder with an average particle size of 150-250 μm (60-100 mesh).

[0067] b. The obtained fine powder is first soaked in hot water at 65°C for 30 minutes, poured out, and then mixed with clean warm water at 40°C and an equal volume of lipase and protease at a ratio of solid:liquid=1:4. Enzyme solution, at a constant temperature of 40°C, slowly mix the leather shell fine powder and crude enzyme solution in a reaction kettle, and carry out a co-enzyme hydrolysis d...

Embodiment 3

[0072] Embodiment 3: Lactobacillus fermentation prepares chitooligosaccharides

[0073] a. Pretreatment: 10 kg of silkworm pupae are cleaned and removed, and the skin is broken, and the content is extruded by pressing, and the skin is collected, dried until the moisture content is less than 10%. It is crushed to a fine powder with an average particle size of 150-250 μm (60-100 mesh).

[0074] b. Soak the fine powder in hot water at 65°C for 30 minutes, pour it off, then add clean warm water at 40°C and an equal volume of lipase and protease mixed crude enzyme solution at a ratio of solid:liquid=1:4 , at a constant temperature of 40°C, slowly mix in the reactor, stir intermittently, and perform a co-enzymatic degreasing and deproteinizing reaction. The reaction time is 18 hours. After the reaction is terminated, centrifuge (2500r / min) for solid-liquid separation.

[0075] c. Collect the solid material, wash it three times with warm water at 40°C, drain it, and wash it with 5% ...

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Abstract

The present invention discloses a method for producing chitin / chitosan from the shell of silkworm chrysalis, fly larvina, which comprises: to break into pieces to progress the shell of silkworm chrysalis, fly larvina by dry or wet method; React the fine grits obtained with the fat enzyme and protein enzyme from micro-organism by a enzymolysis reaction in order to to eliminate the fat and protein contained in the chrysalis / larvina raw material; bleach using two-step method; Collect the materials after bleach and to wash, dry materials to obtain chitin product; Obtain chitosan by to circulate to cast off Acetyl reaction in a Rhizopus oryzae full cell immobilization biology reaction vessel; Obtain height hydrosoluble shel oligosaccharide by liquid submerged fermentation chitosan using acid formers. The present invention improves the quality of the product and the efficiency of production; reduces greatly the energy cost, the usage of acid, alkali, oxidant and other harmful chemistry thing and the discharge of effluent, waste residue; produces byproducts of high value at the same time; utilizes the resources integratedly; and increases the benefits of production.

Description

technical field [0001] The invention belongs to the technical field of biotechnology application, new material and chemical product production, and particularly relates to a method for preparing chitin / chitosan from silkworm chrysalis, fly maggots and other insect skins by biological methods, specifically, The method is to prepare chitin and / or chitosan from insect skin and shell raw materials by co-enzymolysis with various microbial enzymes and biotransformation in an immobilized whole cell bioreactor. Background technique [0002] Chitin, also known as chitin, scientific name chitin (chitin), is a straight-chain natural amino polysaccharide, and it is also the only edible animal fiber with a positive charge found so far. The reserves of chitin in nature are second only to cellulose, and the annual biosynthesis of the biosphere is about 10 billion tons, which is the second largest biological resource on the earth. As a structural substance, chitin is mainly distributed in ...

Claims

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Application Information

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IPC IPC(8): C12P19/04C12R1/125C12R1/645C12R1/72
Inventor 吴力克
Owner CHONGQING LIKE BIOTECH
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