59results about How to "Strong killing effect" patented technology

The invention relates to a formula of an aquatic product composite preservative. The formula of the aquatic product composite preservative is characterized in that the aquatic product composite preservative in each liter of distilled water comprises the following components: 0.4 to 0.6 g of muramidase, 2.5 to 3.5 g of tea polyphenol, 16 to 20 g of carboxymethyl chitosan, 10 to 14 g of nisin, 25 to 35 g of potassium sorbate, 17 to 23 g of sodium alginate, 20 to 30 g of lactobacillus, 1.5 to 2.5 g of propolis extracts and 10 to 14 g of spice extracts; and the spice extracting liquid comprises the raw materials: galangal, garlic, onion, cinnamon, clove and rosemary. The aquatic product composite preservative provided by the invention is prepared by compounding biological preservatives, is safe and non-toxic, has simple preparation and application methods, prominent microbial inhibition effect and good preservation effect, can greatly prolong the shelf life of frozen aquatic products, and has important industrial value.

The invention provides a pinus extract and application thereof. The pinus extract is prepared by a method comprising the following steps of: burning pinus plant fragments; condensing and recycling exhausted gas to obtain coarse liquid; standing the coarse liquid in a closed container for three to five months; removing an upper layer of oily matter and a lower layer of sedimentation; taking a middle layer of liquid to distill; taking distillate of fractions at 98 DEG C to 103 DEG C; absorbing by active carbon, filtering by filter paper (for filtering more than once); and taking filtrate to obtain the pinus extract. The invention provides the pinus extract with multiple medical effects. The pinus extract has a certain treating effect on various diseases, has good effect particularly on human epidermal injury, has assistant treating functions on 00 AIDS and unexpected infectious diseases, and provides a basis for screening out new medicaments.

The invention discloses an in-vitro amplification method of natural killer cells (NK). The in-vitro amplification includes the steps of S1, culturing mononuclear cells separated from peripheral blood with a serum-free medium while adding in IFN-gamma to stimulate for a first preset time; S2, adding class-I cell factors in the serum-free medium for stimulation induction after the first preset time; S3, adding in class-II cell factors in the serum-free medium for induced amplification after culturing for a second preset time, and then performing cell fluid replacement and culturing for a third preset time; and S4, collecting the cells. According to embodiments of the invention, the natural killer cells cultured by the in-vitro amplification method have high killing action and high significant effect to target cells.

The invention belongs to the field of medicinal preparations, and relates to a targeted nanometer delivery release system aiming at a multidrug resistant tumor and a preparation method of the targeted nanometer delivery release system. According to the system and the preparation method, the photosensitizer, namely, pyrophaeophorbide-a (PPA) is bonded to two ends of the copolymer, namely, hydroxylated-polylactic acid-polyglycolic acid (HO-PLA-PEG-PLA-OH) through a chemical bonding method, thus the product, namely, the photosensitizer-embedded polylactic acid-polyglycolic acid polymer (PPA-PLA-PEG-PLA-PPA) is obtained, then the chemotherapy medicine, namely, paclitaxel is encapsulated physically by adopting an emulsified solvent evaporation method, thus the binary-drug-loading nanometer delivery release system (PPA NP-PTX) is prepared, then F3 peptide with the specific targeting function and the cell penetrating function is modified on the surface of the system, and thus the photodynamic therapy and chemotherapy combined drug delivery nanometer delivery release system with the binary targeting property is prepared. The experiment proves that the delivery system has the strong lethal effect for drug resistant tumor cells, has the obvious in-vivo tumor targeting effect, and has the obvious treatment effect for the mice bearing the multidrug resistant tumor, and thus the combined drug delivery nanometer system has the clinical application prospect.

The invention discloses an application method for replacing antibiotics with microbic muramidase in feed. The excellent effect can be obtained by controlling the volume of addition at 60 to 400g / ton feed; and the volume of addition in the feed of different animals is relatively adjusted. By using the method, the inflammation and the infection can be resisted effectively by using the excellent effects of antibiosis and sterilization of the muramidase, the effects of antibiosis and sterilization also have the strong killing effect on bacterial strains generate drug resistance on antibiotics, and the effects of antibiosis and sterilization can ensure that the health of intestinal canals of the animals, prevent and cure the diarrhea of the animals and improve the production performance of the animals. The application method has the advantages of good curative effect, high safety, low cost, no drug resistance, no drug residue and no toxic or side effect, and the microbic muramidase is a green environment-friendly active substance.

The invention discloses bacteriophage pAhMJG and application of the bacteriophage pAhMJG in treatment of fish diseases caused by aeromonas hydrophila. The preservation number of the bacteriophage pAhMJG preserved in the China Center for Type Culture Collection is CCTCC No: M 2019202. The whole length of a bacteriophage pAhMJG genome is 45,057 bp; and the genome is a linear double-stranded DNA andcontains 45 open reading frames. The bacteriophage pAhMJG does not contain sRNA, tRNA or rRNA, also does not contain antibiotic genes, virulence genes or lysogen related genes, has an obvious killingeffect on aeromonas hydrophila, can be used for treating fish diseases caused by aeromonas hydrophila, and has a wide application prospect.

The invention discloses a botanical insecticide, which comprises the following active components extracted from sophora flavescens, tobacco, common monkshood mother roots, kusnezoff monkshood roots, a wormwood, ligusticum, nux vomica, quisqualis indica and croton: 55-70% by weight of matrine, 0.5-5% by weight of tobacco-alkaloid, and 25-44 % by weight of aconitine, ligusticum oil, brucine, potassium quisqualate and crotin. The insecticide belongs to an insecticidal and acaricidal pesticide, can be used for pest control of vegetables, fruit trees, crops and the like, the selected plants are naturally ubiquitous, can be degraded by microorganisms in the nature, have no injury or residues to crops, do not pollute the environment or agricultural products, do not kill natural enemies, have no teratogenic, carcinogenic and mutagenic hazards to mammals, have strong pest-killing effects, are efficient and safe, have good control efficiency and are low in cost. The botanical insecticide is particularly suitable for controlling citrus pests.

The invention discloses a preparation method and application of a photosensitive magnetic nanoparticle system capable of inhibiting growth of breast cancer cells. The preparation method includes: performing amination treatment on magnetic nanoparticles Fe3O4-OA wrapped by oleic acid, and activating carboxyl on a photosensitizer; grafting the photosensitizer onto the surface of Fe3O4-OA through condensation reaction of amino and carboxyl to obtain the photosensitive magnetic nanoparticle system. The photosensitive magnetic nanoparticle system has effect of photodynamically treating breast cancer in the condition of illumination, realizes a combination of breast cancer targeting and folic acid cell targeting of a magnetic field, can effectively inhibit growth of the breast cancer cells in a targeted manner, has the characteristics of high slow release performance, stability, dispersity and uniformity and has the advantage of low toxic and side effect. The system is a novel administration path combining magnetic targeting with nano technology, is of great significance in fully utilizing drug to realize high-efficiency low-toxicity treatment effect and has good application prospect.

The invention discloses application of artemisinin in the preparation of drugs to inhibit or kill breast carcinoma stem cells. Artemisinin can kill breast carcinoma stem cells effectively, can betterkill tumor stem cells than carcinoma cells experiencing no stem evolution, and has selective efficient killing effect against tumor stem cells. Artemisinin can induce 'ferroptosis' of breast carcinomastem cells. By the use of artemisinin with Fe3O4 nano particles, and alternatively further coupling the magnetic nanoparticles with targets, such as tyrosine kinase-like orphan receptor 1 antibody, the effect of killing breast carcinoma stem cells is improved significantly. The invention also discloses an antitumor drug composition to inhibit or kill breast carcinoma stem cells. A new strategy isprovided for tumor therapy to specifically kill tumor stem cells; the application herein is important to the improvement of tumor treatment effect, the reduction of tumor recurrence, the improvementof survival quality of tumor patients and the extending of survival time.

The invention relates to CD8 cytotoxic T-lymphocyte (CTL) for treating lung cancer and a preparation method thereof. The preparation method is characterized by comprising the steps of loading antigen peptide on 668 strains of artificial antigen presenting cell (aAPC), high efficiently activating the artificial antigen presenting cell in vitro, separating CD8T cell from peripheral blood of an HLA-A2 positive tumor patient, activating the CD8T cell to be split into the CD8 cell (CTL) capable of killing the tumor cells, secreting IFN-gamma from the induced CTL, an obvious effect for killing the human tumor cell strains in vitro and self tumor cell in vivo can be realized, the CTL is returned to the patient to treat the tumor cancer or prolong the life of the patient.

The invention discloses a new application of aromatic acid derivative with nitric oxide donor in preparation of medicines for treating malignant cancers. According to the invention, ferulic acid or caffeic acid derivatives with nitric oxide with strong antitumor activities are screened by a plurality of experiments. The experimental results show that the aromatic acid derivative has good effects of inhibiting the growth of tumor cells and promoting tumor cell apoptosis, and also has strong killing function for various malignant cells, and the application range is wider. Especially, three compounds show very strong activity for resisting various tumors, thereby being expected to be developed to new antitumor drugs; and the three compounds are respectively (E)-3-(4-hydroxy-3-methoxyphenyl) propenoic acid-4-[4-benzenesulfonyl-1,2,5-oxadiazole-5-oxy-3-oxy]-2-butyl ester, (E)-3-(4-hydroxy-3-methoxyphenyl) propenoic acid-4-[4-benzenesulfonyl-1,2,5-oxadiazole-5-oxy-3-oxy]-1-butyl ester, and (E)-3-(4-hydroxy-3-methoxyphenyl) propenoic acid-2-{2-[4-benzenesulfonyl-1,2,5-oxadiazole-5-oxy-3-oxy]-ethyoxyl}ethyl ester.

The invention relates to a plastic composite film, in particular to an anti-bacterial medical protective film. The anti-bacterial medical protective film comprises an upper-layer film, an intermediate-layer film and a lower-layer film which are sequentially adhered together, wherein the intermediate-layer film comprises the following ingredients: 10-40 parts of linear low-density polyethylene, 10-40 parts of low density polyethylene and 20-80 parts of metallocene intermediate density polyethylene or metallocene high density polyethylene; the upper-layer film comprises the following ingredients: 10-30 parts of metallocene intermediate density polyethylene or metallocene high density polyethylene, 32-52 parts of low density polyethylene, 30-50 parts of linear low density polyethylene, 1-10 parts of an anti-bacterial agent, 0.5-0.8 part of zeolite and 0.01-0.2 part of a slipping agent. The anti-bacterial medical protective film provided by the invention has a favorable bacterium and fungus killing function, is safe and nontoxic, only needs to be disinfected for a short time before use, or, is directly used without disinfection, and meanwhile, has relatively high transparency.

The invention relates to an external xenoliths vaccine combined dendritic cell cytokine induced killer (DC-CIK) new technology for cancer therapy. Cancer autologous peripheral blood is utilized, mononuclear leucocyte is separated and purified by using a level-100 good manufacturing practice (GMP) workshop, a part of mononuclear leucocyte is induced and differentiated to be dendritic cell (DC), and external xenoliths (EXO) in supernate is collected; and the other part of mononuclear leucocyte is induced into cell factor induced CIK; and DC and CIK are co-cultured simultaneously; and after quality testing passes, cancer patients are actively and passively immunized. EXO is a novel acellular vaccine, so that the EXO is safe, effective, stable and practical; and DC-CIK co-culture has a coordination antineoplastic effect, and costimulatory molecule expression promoting DC is increased; and therefore, cancer treatment through the external xenoliths vaccine combined DC-CIK is a good method for preventing relapse and metastasis of cancers.

The invention discloses a plant type washing-free hand sanitizer. The plant type washing-free hand sanitizer comprises the following components in percentage by mass of 0.4-0.9% of alkyl polyglycoside, 0.2-0.4% of a preservative, 0.1-0.2% of quaternary ammonium salt, 0.01-0.05% of essence, 0.02-0.08% of sodium citrate, 0.005-0.015% of citric acid, 0.5-1.0% of a hydrating agent, 0.5-1.0% of a plantextract and the balance water. The plant extracts are respectively a thyme extract, a purple perilla extract, a common lantana leaf extract and a railway beggarticks extract, and the mass ratio of the hydrating agent to the thyme extract to the purple perilla extract, to the common lantana leaf extract to the railway beggarticks extract is 5:2:1:1:1. The washing-free hand sanitizer can realize efficient long-acting sterilization, can restrain breeding of bacteria, can effectively protect skin safety, and has the effects of washing and moisturizing skin. The preparation method is simple, easyfor mass production and free from pollution in the preparation process.

The invention discloses an insecticide aerosol. The aerosol comprises the following components in percentage by weight: 0.01 to 0.2 percent of imiprothfin, 0.1 to 0.3 percent of ES-bioallethrin, 0.1 to 0.3 percent of d-phenothrin, 0.4 to 1.5 percent of octacide 264, 0.4 to 2.0 percent of butoxide, 29 to 68 percent of organic solvent and 29 to 68 percent of propellant, wherein the octacide 264 is N-(2-ethylhexyl) bicyclo-[2,2,1] heptyl-5-alkene-2,3-dicarboximide; and the butoxide is 3,4-methylene dimethoxy-6-propylbenzyldiethyleneglycol butyl ether. The insecticide aerosol has good effects on killing, preventing and controlling a plurality of pests such as mosquitoes, flies, cockroaches, ants, fleas, house dust mites and the like, has the characteristics of high efficiency, broad spectrum, low toxicity and no residue and is safe to human beings and livestock and compatible to the environment.

The invention discloses a porcine compound astragalus polysaccharide injection and a preparation method thereof. The invention aims to provide a compound astragalus polysaccharide injection which treats the diarrhea caused by compound infection of porcine bacteria and viruses, has quick response, treats both principal and secondary aspects of diseases, reduces the applied times of the medicament and has convenient medicament use, and a preparation method with simple process and easy realization. Each 100L of injection comprises 0.5 to 5kg of astragalus polysaccharide, 1 to 7kg of maquindox, 3 to 21kg of sodium salicylate, 2 to 10kg of colistin sulfate, 0.18kg of atropine sulfate, 30kg of propanediol, 5kg of 95 percent ethanol, and the balance of water for injection. The injection synchronously takes effect from many aspects through the reasonable proportioning of the consumption of various components of several medicaments, has quick response on the treatment of the diarrhea caused by the compound infection of the porcine bacteria and viruses, treats both principal and secondary aspects of diseases, reduces the drug resistance of bacteria, improves the resistance of organisms, and ensures that the recovery of pigs is accelerated. At the same time, a compound preparation of the invention is the injection and has convenient use.

The invention relates to application of demethylzeylasteral to preparation of a medicine for treating pancreatic cancer. The application proves that the compound demethylzeylasteral has a significant killing effect on human pancreatic cancer cells, can induce tumor cell cycle arrest in a G0 / G1 phase and achieves a pancreatic cancer resisting effect through induction of autophagic death and Caspase-3-dependent apoptosis of the cells; in combination with gemcitabine, the demethylzeylasteral can significantly reduce the IC50 of the gemcitabine, and through combined drug administration, a better inhibitory effect on the human pancreatic cancer cells is achieved; at low concentration, the demethylzeylasteral can induce the autophagic death of the cells so as to improve the antitumor effect of the gemcitabine, while at high concentration, the demethylzeylasteral improves the chemotherapeutic effect of the gemcitabine mainly through promotion of the apoptosis. On the basis of the application, the demethylzeylasteral can be applied to preparation of the medicine for treating the pancreatic cancer, and the prepared medicine is combined with the gemcitabine for use, or the demethylzeylasteral can be used for researching a cell autophagy or apoptosis mechanism.

The invention discloses a gold nanometer podophyllotoxin liposome, a preparation method and use thereof. The gold nanometer podophyllotoxin liposome is a nano-composite obtained by causing a gold nanorod to be modified by a polyelectrolyte and then combined with the outer surface of the podophyllotoxin liposome containing carboxyl. The gold nanometer podophyllotoxin liposome is in a typical vesicle shape with a particle diameter of about 129.8 nm and a zeta potential of +15.7 mV, and has a high extinction coefficient (8.08Lg<-1>cm<-1>) and photothermal conversion efficiency (46.1%); and the gold nanometer podophyllotoxin liposome has no cytotoxicity and no hemolysis. The gold nanometer podophyllotoxin liposome has good photothermal stability under near-infrared irradiation and can achieverapid release; and the synergistic treatment of chemotherapy-photothermal therapy has a strong killing effect on tumor cells, and an effect thereof may not be limited to this.

The invention discloses a preparation method of an environment-friendly deodorant. The preparation method comprises the following steps: A, feeding hot water into a preparation device, separately adding sodium benzoate and potassium sorbate, and performing stirring and mixing until uniformity to obtain a primary mixed solution; B, cooling the primary mixed solution to room temperature, adding benzalkonium bromide and a stabilizer, and performing stirring and mixing until uniformity to obtain a secondary mixed solution; and C, metering and packaging the secondary mixed solution to obtain the deodorant. The preparation method of the environment-friendly deodorant has the advantages of simple steps, and convenience in operation, and the prepared deodorant has the advantages of long deodorization duration, fundamental removal of odor, and good deodorization effect.

The invention discloses an antibacterial filter core and its preparation method and application. The antibacterial filter core has a strong antibacterial effect, has a strong killing effect on Escherichia coli and Staphylococcus aureus in a short time and is suitable for water purification. The antimicrobial filter core comprises nanometer copper-carbon composite particles and cellulose. A weight ratio of the nano-copper-carbon composite particles to the cellulose is (8-12): 1. The particle sizes of the nano-copper-carbon composite particles are 20 to 50nm. The preparation method comprises uniformly mixing a nano-copper-carbon composite material and cellulose, putting the mixture in a mold cavity, carrying out calcination and carrying out cooling, drying and molding.

The invention discloses mildew-proof glass glue and a preparation method thereof and relates to the technical field of glass processing. The mildew-proof glass glue is prepared from raw materials as follows: 10-20 parts of dichloromethane, 15-25 parts of methyl silicone, 5-7 parts of polyacrylate, 8-10 parts of polyurethane, 4-8 parts of hydroxyl silicone, 3-5 parts of a plasticizer, 5-7 parts of methyl polytetrafluoroethylene, 6-8 parts of calcium carbonate, 6-8 parts of titanium dioxide, 3-5 parts of benzoyl peroxide, 6-8 parts of silicon dioxide and 2-4 parts of a modifier. The mildew-proof glass glue has the benefits as follows: the technological procedure is concise, the proportion of raw materials is reasonable, the raw materials are cheap and easy to obtain, the product has very high adhesion force and lasting flexibility, the mildew-proof effect is improved due to addition of methyl silicone and oxidized polyethlene wax, and the mildew-proof glass glue is application to occasions where mildew is easily bred and has strong repelling and killing functions on various bacteria.

The invention discloses 2,3-epoxy-2-propyl sulfone-5,8-dimethoxy-1,4-naphthoquinone as well as a preparation method thereof, and a medicine containing 2,3-epoxy-2-propyl sulfone-5,8-dimethoxy-1,4-naphthoquinone, and belongs to the technical field of medicines. The structural formula is as shown in the description. The method comprises the following steps: I, dissolving 5,8-dimethoxy-1,4-naphthoquinone in methyl alcohol, adding 1-propyl sulfhydrate for reaction at room temperature, then adding sodium bichromate and concentrated sulfuric acid for continuous reaction, carrying out extraction withdichloromethane and a saturated saline solution, drying the product with anhydrous sodium sulfate, filtering the product, and concentrating the product till the product is dry; II, dissolving the product in chloroform, continuously adding 3-chloroperoxybenzoic acid for reaction at room temperature, adding a NaHCO3 solution at the concentration of 5 percent after the reaction is completed to neutralize surplus metachloroperbenzoic acid in the reaction, then ending the reaction, carrying out extraction with the dichloromethane and the saturated saline solution, drying the product with the anhydrous sodium sulfate, filtering the product, and concentrating the product till the product is dry. The compound and a medicine composition can be applied to preparation of a medicine for treating a liver cancer.

The invention discloses an enhanced anti-tumor fusion protein, a preparation method and use thereof. The fusion protein includes at least two of a costimulatory molecule B7.1 that induces and activates T cells, a costimulatory molecule B7.2 that induces and activates the T cells, and a superantigen Staphylococcus aureus enterotoxin A. The fusion protein of the present invention constructs at leasttwo of B7.1, B7.2 and SEA into the fusion protein, and exerts the superimposed amplification effect. The biological activity is far more than that of a single source protein, the T cells are inducedand activated to kill tumors, the T cells secrets cytokines such as tumor necrosis factor alpha (TNF-alpha) and granzymes and induces the cancer cells as target cells to produce Fas and the like to cause cancer cells to apoptosis, The experiments have shown that each of the fusion proteins of the present invention has the significant killing effect on various cancers and solid tumors.

The invention discloses a preparation method of a medical film having a sterilizing function. The preparation method comprises the following steps: stirring and uniformly mixing PVC, phenolic resin, PET, stearate, polyvinyl alcohol and paraffin in a stirrer to obtain a mixture I, then transferring the mixture I into a reaction kettle, adding sodium dodecyl benzene sulfonate, melamine, polyacrylamide and hydroxypropyl methyl cellulose into the reaction kettle, heating and stirring under a negative pressure condition, then cooling to the room temperature, adding an antioxidant, an auxiliary antioxidant and barium stearate, uniformly stirring and mixing to obtain a mixture II, extruding and granulating the mixture II in a dual-screw extruder to obtain a master batch; finally extruding and blowing the master batch by adopting a plastic film blowing machine to obtain the medical film having the sterilizing function. The medical film prepared in the method is good in sterilizing performance and physical performance and wide in application prospect.

The invention provides a health-care traditional Chinese medicine scented sachet, which is characterized in that medicines in the scented sachet do not have the function of directly killing virus and bacteria, but give off persistent aromatic odor, which stimulates the human body to generate secreted immunoglobulin A, and such an antibody has a strong action of killing virus and bacteria, thus achieving the health-care effect.

The invention relates to a dihydroporphin photosensitizer as well as a preparation method and an application thereof. The dihydroporphin photosensitizer is meso-4(3-N,N-diethylaminemethyl)-4-metoxyphenyl)-dihydroporphin, and the chemical structural formula thereof is provided. The photosensitizer is prepared by the following steps: reducing meso-4(3-N,N-diethylaminemethyl)-4-metoxyphenyl)-dihydroporphin through a reducer in the presence of nitrogen gas and eluting through silica gel column chromatography. The dihydroporphin photosensitizer provided by the invention is used for preparing medicines for treating tumors and photoactivated insecticides. The preparation method is simple. The dihydroporphin photosensitizer can be made into hydrochloride injections to use, and has wide application prospects.

The invention discloses application of Fenghuang Dancong tea extract in preparation of anti-breast-cancer drugs. The Fenghuang Dancong tea extract is acquired by means of water extraction; water-soluble active ingredients in the Fenghuang Dancong tea extract have good antitumor activity; the Fenghuang Dancong tea extract is highly worthy of research for human breast cancer. Raw material for the Fenghuang Dancong tea extract herein is low in price and easy to obtain; the extraction process is simple and convenient and has simple conditions; the Fenghuang Dancong tea is safe to human; the Fenghuang Dancong tea extract is expectedly promotive in the application of Fenghuang Dancong tea and its isogeneic tea drinks in the fields such as anti-breast-cancer medicine, cosmetics, health products and foods; the Fenghuang Dancong tea extract is also applicable to the fields such as foods, health products and biomedicine to resist oxidation and improve body immunity.

The invention relates to a marine microorganism adhesion-resistant olefin composite material and a preparation method thereof. The preparation method comprises the following steps: (1) weighing a copolymerized olefin material, an inorganic nano component and an olefin monomer with a cyclopentadiene structure, performing blending, and carrying out ultrasonic dispersion treatment to obtain a blend;and (2) adding the blend obtained in the step (1) and a catalyst into a reactor, mixing the substances uniformly, injecting the mxiture into a mold, and conducting polymerizing, curing and molding toobtain a target product. Compared with the prior art, the prepared olefin composite material has excellent mechanical properties like good wear resistance, high strength, high toughness and the like,and more importantly, adhesion of marine microorganisms can be effectively inhibited, and the olefin composite material has excellent marine corrosion resistance and is beneficial to application of the composite material in the field of ocean engineering.

The invention relates to the technical field of pesticides, in particular to novel chlorine-containing bifenthrin, a preparation method thereof and application of the novel chlorine-containing bifenthrin in anti-mosquito drugs. The novel chlorine-containing bifenthrin is as shown in structural formula I. The preparation method includes: adding an intermediate A as shown in structural formula II and a compound B as shown in structural formula III into solvent toluene, adding potassium phosphate, and adding a catalyst to perform reaction to obtain the novel chlorine-containing bifenthrin. By thenovel chlorine-containing bifenthrin and the preparation method and application thereof, the technical problems that existing pyrethroid in the prior art easily generates potential toxicity and is poor in pesticide effect are solved.

The invention relates to a pesticide and a preparation method thereof. According to the pesticide, cucurbit leaves, apricot leaves, halogeton arachnoideus and garlic root hairs are adopted as raw materials and subjected to proper weight ratio, thus the pest-killing functional components of all the raw materials interact and are synergistically enhanced, the pesticide has the significant killing effect on crop pests and has the broad-spectrum effect. According to the preparation method of the pesticide, by optimizing the preparation process, the extracting efficiency is significantly improved,and active components are better reserved. In addition, the pesticide is extracted from natural plants, environmental protection is achieved, the toxic and side effects on the human body are avoided,using is safer, and the preparation method is simple, the cost is low, and the pesticide is suitable for industrial production.