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CD8 cytotoxic T-lymphocyte for treating lung cancer and preparation method thereof

A technology of lymphocytes and cells, which is applied in the field of CD8 toxic T lymphocytes (CTL) and its preparation, can solve the problems of low tumor killing specificity and unclear killing mechanism, and achieve the effect of prolonging life

Active Publication Date: 2014-03-26
SHANGHAI YUYAN BIOTECH CO LTD
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

However, the specificity of tumor killing is low and the killing mechanism is not very clear

Method used

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  • CD8 cytotoxic T-lymphocyte for treating lung cancer and preparation method thereof
  • CD8 cytotoxic T-lymphocyte for treating lung cancer and preparation method thereof
  • CD8 cytotoxic T-lymphocyte for treating lung cancer and preparation method thereof

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Embodiment Construction

[0022] In order to make the present invention clearer, the present invention will be further described in conjunction with the accompanying drawings and embodiments. It should be understood that this embodiment is not intended to limit the protection scope of the present invention.

[0023] 1) Preparation and analysis of peptides: Using bioinformatics methods, analyze the antigenic peptide fragments in lung cancer-specific antigen molecules, design peptides that can bind to MHC class I molecules, and synthesize these peptides in vitro to ensure the purity of above 95. To study the binding stability of peptides to MHC class I molecules.

[0024] 2) Artificial antigen-presenting cells:

[0025] (1) Preparation of artificial antigen-presenting cells (aAPC) from insect cells: construct vectors expressing various related proteins. Based on Drosophila S2 cells, HLA-A2, β-microglobulin, CD80, and intercellular adhesion molecule-1 (ICAM-1, CD54) were co-transfected to express; flow ...

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Abstract

The invention relates to CD8 cytotoxic T-lymphocyte (CTL) for treating lung cancer and a preparation method thereof. The preparation method is characterized by comprising the steps of loading antigen peptide on 668 strains of artificial antigen presenting cell (aAPC), high efficiently activating the artificial antigen presenting cell in vitro, separating CD8T cell from peripheral blood of an HLA-A2 positive tumor patient, activating the CD8T cell to be split into the CD8 cell (CTL) capable of killing the tumor cells, secreting IFN-gamma from the induced CTL, an obvious effect for killing the human tumor cell strains in vitro and self tumor cell in vivo can be realized, the CTL is returned to the patient to treat the tumor cancer or prolong the life of the patient.

Description

[technical field] [0001] The invention relates to CD8 toxic T lymphocytes (CTL) and a preparation method thereof, in particular to CD8 toxic T lymphocytes (CTL) for treating lung cancer and a preparation method thereof. [Background technique] [0002] (1) Adoptive immunotherapy with autologous cells [0003] The emergence of autologous cell immunotherapy makes up for the shortcomings of the three traditional tumor treatments—radiotherapy, chemotherapy, and surgery—that it is difficult to completely wipe out tumor cells. It uses its own cells to treat its own disease, and improves the function of the patient's own immune system. Enhance the differentiation ability of specific tumor-killing cells, and kill tumors at the cellular level without any side effects. This "autonomous anti-cancer" therapy that realizes the patient's own body is expected to completely eliminate and cure cancer cells in the body, and will become a major direction of cancer treatment in the future. Thi...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0783A61K35/14A61P35/00A61K35/15
Inventor 施炜星杨春霞房永生
Owner SHANGHAI YUYAN BIOTECH CO LTD
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