The invention discloses a mask capable of removing acne, whitening skin and achieving nourishing and a preparation method of the mask. The mask is prepared from aloes, radix et rhizoma rhei, herba opuntiae, fructus gardeniae, semen plantaginis, fructus forsythiae, rhizoma alismatis, bitter melon juice, hyaluronic acid, honey, vitamin E, milk, pearl powder and kudzuvine root starch. The aloes, radix et rhizoma rhei, herba opuntiae, fructus gardeniae, semen plantaginis, fructus forsythiae and rhizoma alismatis are washed, air-dried, disinfected at high temperature, smashed and mixed, an alcohol-water system serves as solvent, heating extraction is conducted, the bitter melon juice, hyaluronic acid, the honey and the milk are added to be mixed and stirred, the rest substances are added then, stirring and filtration are conducted, heating and heat preservation are conducted, cooling is conducted to room temperature, concentration and evaporation are conducted till the mixture becomes thick paste, the thick paste is poured into a mask mold, and the mask is obtained. The prepared mask has an excellent acne removing effect, the effect is prominent, the formula is gentle, stimulation to skin does not exist, and the mask further has the effects of whitening the skin and nourishing the skin.