44results about How to "High sporulation rate" patented technology

The invention provides a composite bacillus preparation containing three strains, a preparation method of the composite bacillus preparation and application of the composite bacillus preparation to ecological breeding, and belongs to the technical field of microorganism and feed additives. Bacillus coagulans, butyric acid clostridia and bacillus megaterium are used as fermentative strains respectively, and large-scale, low-cost and high-density fermentation production of spores is conducted through step-by-step magnification; three live bacterium preparations are prepared by mixing bacterial sludge with the spores and dry starch; the three live bacterium preparations are matched according to the equal weight ratio, the composite bacillus preparation is obtained, and the concentration of the spores reaches more than 1*108-109 cfu/g; the composite bacillus preparation serves as a microorganism and feed additive to be added to farmed animal feed according to the mass ratio ranging from 0.02% to 0.5%. The composite bacillus preparation is simple and stable in process, low in cost, high in spore content and environment tolerance, easy to store and high in survival rate; the purposes of composition and synergism are achieved through the collaborative supplementary effects of different bacilli in the aspect of micro-ecology adjustment, growth of farmed animals is promoted, the feed utilization rate and feed rewards are increased, diarrhea is prevented and reduced, and the breeding ecological environment is improved.

The combination of probiotics and food is helpful for inhibiting the growth of pathogens and absorbing special nutritions. However, the probiotics need to survive at high temperature in the process of food processing, and after the probiotics enter the body, the probiotics must go through the test of stomach acid and bile salt to reach the intestines. Accordingly, the invention provides a bacillus subtilis strain BS139, which is characterized by the survivability of resisting environmental stresses, including the characteristics such as acid resistant, bile salt resistant, high temperature resistance and the like. The probiotic strain is preserved in Agricultural Research Service, NRRL and has a preservation number of NRRL No.B-50347, with further applications in the preparation of food commodities, pharmaceutical compositions, and daily necessities.

The invention provides a streptomycesvinaceus-drappus microbial inoculum. The streptomycesvinaceus-drappus microbial inoculum uses an active bacteria culture prepared by liquid submerged fermentation or solid fermentation of an active strain of streptomycesvinaceus-drappus, of which the storage number is CGMCC No. 2664, as an active ingredient. A tentative name of the streptomycesvinaceus-drappus microbial inoculum is S506, and the streptomycesvinaceus-drappus microbial inoculum was submitted to the CGMCC for storage on 11st September, 2008. The preparation method comprises the following steps of: A, activation of the strain; B, preparation of liquid seeds of the streptomycesvinaceus-drappus; and C, liquid submerged fermentation of the streptomycesvinaceus-drappus. The invention also provides a novel process of 'liquid seed preparation and solid fermentation' which is favorable for the growth of the streptomycesvinaceus-drappus, and the novel process meets the technical requirements on high effective viable count, high spore production rate, light contamination of crossbred bacteria and short fermentation period. When the active streptomycesvinaceus-drappus is applied to the roots of vegetables, various root diseases, such as blight, root rot and root knot nematode disease, of vegetables can be effectively prevented and controlled, and the streptomycesvinaceus-drappus microbial inoculum has obvious effects of enforcing growth of root systems and degrading autopoisonous matters of root systems, so that the streptomycesvinaceus-drappus microbial inoculum has a wide application prospect in effectively solving the problem of continuous cropping obstacles which are commonly happed to vegetables, fruit trees, medicinal materials and the like.

The invention belongs to the field of veterinary parasites, and relates to coccidian oocyst culture preservative fluid and an application method thereof. The invention is characterized in that the culture preservative fluid takes polyhaxemethylenguanidine hydrochloride as a main ingredient. The application method of the coccidian oocyst culture preservative fluid comprises the following steps of: separating and purifying fresh coccidian oocysts from fowl dung or intestinal tract tissues containing the coccidian oocysts; adding an appropriate amount of a polyhaxemethylenguanidine hydrochloride solution; adjusting the density of the oocysts; controlling the depth of a culture medium; putting the culture medium in a shaking table to perform shaking culture; performing microscopic examination; stopping culture when the sporulation rate of the oocysts exceeds 85%; collecting the oocysts; adding an appropriate amount of the polyhaxemethylenguanidine hydrochloride solution again; and preserving in a refrigerator of which the temperature is 4 DEG C. The coccidian oocyst culture preservative fluid is efficient, long-acting and safe, pollution-free to the environment, simple to prepare and low in cost, and is ideal coccidian oocyst culture preservative fluid. By applying the application method provided by the invention, the operation is simple and convenient, the efficiency is high, the automation can be realized, and the coccidian oocyst culture preservative fluid is suitable for large-scale culture preservation of coccidian oocysts.

The invention provides a production method for bacillus licheniformis with a high sporation rate. According to the production method, no manganese chloride is added into a fermentation medium in a fermentation tank, a sterilized manganese chloride solution is supplemented after fermentation is carried out for 23-25 h, and the volume of the sterilized manganese chloride solution accounts for 0.03-0.04 % of the fermentation volume. As manganese chloride is added in the middle and later stage of fermentation, early-stage growth of the bacillus licheniformis is not affected, the sporation rate of the bacillus licheniformis is increased, the fermentation cycle is shortened, the fermentation production cost is reduced, and better product quality is achieved.

The invention discloses a solid fermentation method of bacillus coagulans. The method comprises the following steps of: (1) putting the bacillus coagulans in a seed culture solution for recovery passage so as to obtain a seed bacterium solution; (2) inoculating the prepared seed bacterium solution in a sterile solid culture medium for fermental cultivation so as to obtain zymophyte; and (3) drying the prepared fermentation products at 60 DEG C in vacuum so as to prepare finished fermentation bacillus coagulans product. The solid fermentation method of bacillus coagulans disclosed by the invention has the beneficial effects that the fermentation time is 24 hours and is saved, the fermentation period is shortened, besides, the cell concentration is large, spore formation rate is high, common fermentation matrix is used, so the storage and transportation are convenient; not only are no waste liquid and environmental pollution caused, but also the cost is low.

The invention relates to the technical field of insect biological control, in particular to a locust microsporidia AL200801 strain as well as a biological preparation and application of the locust microsporidia AL200801 strain. The invention provides a locust microsporidia AL200801 strain, and the preservation number of the locust microsporidia AL200801 strain is CCTCC No. V202137. The locust microsporidia AL200801 strain disclosed by the invention has the advantages of high timeliness, high lethality, high sporulation rate, no toxicity and strong obligate property.

The invention relates to a paecilomyces lilacinus strain PNVT8 for expressing the hemoglobin VHb of vitreoscilla and the application of the paecilomyces lilacinus strain PNVT8. The recombinant paecilomyces lilacinus strain PNVT8 is collected in the China center for type culture collection with the collection number of CCTCC No:M 2013296. The paecilomyces lilacinus strain PNVT8 is obtained by optimizing a codon, a promoter and a gene structure, successfully integrating the hemoglobin VHb from the vitreoscilla into a chromosome of a wild paecilomyces lilacinus strain ACSS which has a plant parasitic nematode killing effect and is provided by the agricultural culture collection of China, and performing screening. Compared with the wild paecilomyces lilacinus strain ACSS, the paecilomyces lilacinus strain PNVT8 has the advantages that more extracellular proteases, chitinase, alpha-amylases and the like can be generated, and meanwhile, the biomass and the cell yield are improved; after the paecilomyces lilacinus strain PNVT8 is applied as a product, the growth rate during field planting under a soil micro-aerobic condition also is increased to further improve the nematode larva killing activity.