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Chicken coccidial oocyst protective solution and preparation method thereof

A chicken coccidia oocyst and protection solution technology, which is applied in the field of chicken coccidia oocyst protection solution and its preparation, can solve the problem of low sporulation rate, non-compliance of safety and efficacy testing, and impact on human and animal health, etc. problem, achieve the effect of increasing sporulation rate, low cost, and improving vitality

Active Publication Date: 2017-08-08
TIANJIN HLINTE BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Due to environmental protection and food safety issues, the Ministry of Agriculture stipulates that the addition of antibiotics and antiparasitic drugs to feed is prohibited, and antibiotic-free feed is gradually realized. In recent years, the use of drugs, especially sulfonamides, has been reduced in the prevention and treatment of coccidiosis. Gradually develop towards the use of coccidiosis vaccines. At present, the protective solution of coccidiosis vaccines at home and abroad basically uses potassium dichromate solution, polyhexamethylene guanidine hydrochloride solution, chloramine T and chloramine B solution, sucrose solution, etc. as spores Culture culture and protection solution, but the sporulation rate is not high, and long-term storage will lead to safety and efficacy testing does not meet the requirements
Potassium dichromate solution is a metal compound, long-term use will remain in the animal body and the environment, affecting the health of humans and animals. Therefore, it is necessary to seek a green, environmentally friendly, safe and long-lasting chicken coccidian oocyst protection solution in the future. Priorities for research and development of parasite vaccines

Method used

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  • Chicken coccidial oocyst protective solution and preparation method thereof
  • Chicken coccidial oocyst protective solution and preparation method thereof
  • Chicken coccidial oocyst protective solution and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] A chicken coccidian oocyst protection solution, the components and weight of the protection solution are respectively: 10 kg of chlorhexidine acetate, 40 kg of sodium sodium benzoate, 10 kg of sodium chloride, 30 kg of beta-mannan, and 80 kg of sterilized water.

[0024] A chicken coccidian oocyst protection solution and a preparation method thereof, the steps of the preparation method are:

[0025] (1) Weigh chlorhexidine acetate, sodium benzoate, sodium chloride, and β-mannan in sequence according to the weight ratio and put them into the liquid mixing tank, then add sterilized water until the above-mentioned materials are covered, and soak at room temperature for 3 After one hour, continue to add the remaining sterilized water, stir evenly until the feeding material is completely dissolved, then filter with a 0.45um polyethersulfone filter element to obtain solution A, and store it in the liquid mixing tank;

[0026] (2) It is advisable to heat the liquid distributio...

Embodiment 2

[0030] A chicken coccidian oocyst protection solution, the components and weight of the protection solution are respectively: 5 kg of chlorhexidine acetate, 10 kg of sodium sodium benzoate, 5 kg of sodium chloride, 10 kg of β-mannan, and 10 kg of sterilized water.

[0031] The steps of its preparation method are the same as in Example 1.

Embodiment 3

[0033] A chicken coccidian oocyst protection solution, the components and weight of the protection solution are respectively: 8 kg of chlorhexidine acetate, 8 kg of sodium sodium benzoate, 6 kg of sodium chloride, 20 kg of beta-mannan, and 60 kg of sterilized water.

[0034] The steps of its preparation method are the same as in Example 1.

[0035] Clinical application

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Abstract

The invention relates to a chicken coccidial oocyst protective solution and a preparation method thereof. The composition consists of the following components in parts by weight: 5-10 parts of chlorhexidine acetate, 10-40 parts of sodium benzoate, 5-10 parts of sodium chloride, 10-80 parts of sterilized water and 10-30 parts of [beta]-mannase. The protective solution provided by the invention is scientific and reasonable in formula composition, simple in process, convenient to use and relatively low in cost; the protective solution, which takes such ingredients as the chlorhexidine acetate and the like as chicken coccidial oocyst protective liquid, can effectively improve a sporation rate of chicken coccidial oocysts; and when chicken E.tenella, E.acervulina, E.maxima and E.necatrix coccidial oocysts are preserved within 6 months, activity is free from any influence, and the chicken E.tenella, E.acervulina, E.maxima and E.necatrix coccidial oocysts, when preserved within 8 months, conform to requirements based upon safety test and efficacy test.

Description

technical field [0001] The invention belongs to the field of veterinary biological products, in particular to a chicken coccidia oocyst protection solution and a preparation method thereof. Background technique [0002] Due to environmental protection and food safety issues, the Ministry of Agriculture stipulates that the addition of antibiotics and antiparasitic drugs to feed is prohibited, and antibiotic-free feed is gradually realized. In recent years, the use of drugs, especially sulfonamides, has been reduced in the prevention and treatment of coccidiosis. Gradually develop towards the use of coccidiosis vaccines. At present, the protective solution of coccidiosis vaccines at home and abroad basically uses potassium dichromate solution, polyhexamethylene guanidine hydrochloride solution, chloramine T and chloramine B solution, sucrose solution, etc. as spores Culture culture and protective solution, but the sporulation rate is not high, and long-term storage will lead t...

Claims

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Application Information

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IPC IPC(8): C12N1/04C12R1/90
CPCC12N1/04
Inventor 陈庆忠安同伟曹桂敏李迎梅
Owner TIANJIN HLINTE BIOTECH CO LTD
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