Bacillus coagulans strain and application thereof

A technology of bacillus coagulans and strains, applied in the field of microbiology, can solve the problems of unreachable effects and discounted probiotic effects, and achieve the effects of favorable spore formation, high spore formation rate, and good heat resistance

Pending Publication Date: 2020-06-09
HANGZHOU BIOCOM BIOLOGICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, even if the application of Bifidobacteria and Lactobacillus can be kept in a low-temperature storage environment, the probiotic effect will be greatly reduced after being ingested by the human body, and it will not be able to achieve the effect of various product packaging promotions

Method used

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  • Bacillus coagulans strain and application thereof
  • Bacillus coagulans strain and application thereof
  • Bacillus coagulans strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] Hangzhou Baoankang Biotechnology Co., Ltd. screened out a strain of Bacillus coagulans (Bacillus coagulans), whose preservation number is CGMCC No. 17374, which is a new strain.

[0060] Its fermentation process is as follows:

[0061] (1) Primary slant culture of Bacillus coagulans:

[0062] The original bacteria were inoculated on the primary slant medium, and cultured at 30°C-45°C for 20-48h. The primary slant medium is a mixed solution (g / L) prepared according to the following ratio: yeast powder 6.0-15.0 g / L peptone 6.0-15.0 g / L glucose 2.0-10.0 g / L sodium chloride 5.0-10.0 g / L dipotassium hydrogen phosphate 1.0~5.0g / L manganese sulfate 0.1~3.0g / L pH 6.6~7.2

[0063] (2) Secondary culture of Bacillus coagulans:

[0064] Inoculate the single colony of the primary Bacillus coagulans in the secondary culture medium in the step (1), the liquid volume is 50-100mL / 200mL / 300mL / 500mL, and cultivate under the conditions of 30°C-45°C and 150-300rpm / min After 20-48 hours,...

Embodiment 2

[0074] Bacillus coagulans strain (Bacillus coagulans), its preservation number is CGMCC No.17374, the application of this strain in the direction of microbial inoculation agent.

[0075] details as follows:

[0076] (1) Collect the solid fermentation product of Bacillus coagulans in Example 1 and dry it at a temperature of 40-60°C for 18-36 hours until the water content reaches 5-10%.

[0077] (2) The dried product in step (1) is pulverized, and the particles are passed through a 60-mesh sieve, collected and bagged, sealed and stored in a cool and dry place.

[0078] (3) Determining the amount of viable Bacillus coagulans and the number of spores of the microbial agent in step (2).

[0079] (4) Based on the measurement results in step (3), the powder in step (2) is quantitatively packed into the capsule shell, and the quantification of live bacteria is 1.5×10 8 ~3.0×10 8 CFU / capsule.

[0080] The above-mentioned capsules are commercially available medicinal capsule shells....

Embodiment 3

[0083] The microbial preparation capsule described in Example 2 was tested for biological efficacy.

[0084] 1. Subject volunteers 50 people voluntarily participated in this research and signed the informed consent.

[0085] Inclusion criteria:

[0086] Inclusion criteria: (1) have long-term or short-term constipation, laxative-dependent constipation, seasonal or common diarrhea, alcoholic colitis, abdominal distension and other symptoms of intestinal diseases; (2) aged 8 to 60 years old; (3) voluntarily participate in this Study, signed informed consent.

[0087] 2. Method

[0088] 1. Research method: The volunteers were tested for symptoms of intestinal diseases, and 50 people who met the inclusion criteria were selected, and they were given the Bacillus coagulans microbial preparation capsules described in Application Example 1, and a comparative test was carried out for 60 days. And continue to pay attention to the effect after stopping taking it.

[0089] 2. Evaluatio...

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Abstract

The invention discloses a Bacillus coagulans strain and application thereof. A preservation number of the Bacillus coagulans strain is CGMCC No.17374. The Bacillus coagulans strain is applied to microbial preparation field. The screened strain has the characteristics of highly producing spores and acid; the strain has obvious difference with other congeneric strains, formation rate of the spores is very high, the heat resistance is good, and the fermentation level is high; the strain is a probiotic and has the effects of maintaining balance of intestinal tracts, stimulating the immune and improving the health level of bodies same as normal lactic acid bacteria. A capsule preparation prepared by taking the strain as a core component has a good treatment effect and a function of regulating multiple intestinal discomforts.

Description

technical field [0001] The invention relates to the field of microbiology, in particular to a bacillus coagulans strain and application thereof. Background technique [0002] The gut is known as the second brain of the human body, and the importance of gut health is self-evident. In terms of intestinal function, in addition to the common digestion and nutrient absorption, there are immune and detoxification functions that have been ignored by most people. A person consumes about 70 tons of food in his life, which is more than 1,000 times his body weight, and these foods are processed by the intestines. The total length of the adult large intestine and small intestine is about 6 to 7 meters. It is an important organ for our nutrient absorption. 99% of the various nutrients needed by the human body are absorbed from the intestinal tract. Furthermore, the intestine is the largest immune organ in the human body. Numerous immune cells such as intestinal dendritic cells and some...

Claims

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Application Information

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IPC IPC(8): C12N1/20A61K35/742A61P1/10A61P1/12A61P1/14A61P1/00C12R1/07
CPCC12N1/20A61K35/742A61P1/10A61P1/12A61P1/14A61P1/00C12R2001/07C12N1/205
Inventor 王云龙吴勃王天珍徐永雷王云祥
Owner HANGZHOU BIOCOM BIOLOGICAL TECH
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