Method for preparing composite biological fertilizer by combined solid fermentation of double bacterium
A compound biological bacteria, solid fermentation technology, applied in the direction of organic fertilizers, etc., can solve the problems of unstable product performance, long fermentation cycle of bacteria, low spore formation rate, etc., to reduce the probability of bacterial contamination and spore formation rate. The effect of high and lower production cost
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Embodiment 1
[0009] Embodiment 1 prepares secondary jelly-like bacillus seeds
[0010] 1. Cultivate jelly-like Bacillus (bacteria are purchased from the strain preservation center of Beijing Institute of Microbiology, Chinese Academy of Sciences) first-class seeds by shaker flasks, shaker temperature 26-30 ° C, shaker speed 180-200 rpm, The pH value is 7.7-7.9, and the growth cycle is 14-15 hours.
[0011] In the primary medium formula, the weight percentage of each component is as follows: 84% starch, 0.85% ammonium sulfate, 15% NaCL, and 0.15% sucrose.
[0012] 2. Place the secondary seed culture medium in the seed tank and perform sterilization treatment. The sterilization temperature of the seed tank liquid is 121 ° C, the holding time is 30 minutes, and the pH value of the seed liquid after sterilization is 7.7-7.9; then the primary The seeds are inoculated into the seed tank, so that the temperature in the seed tank is controlled at 28-30 ° C, and the tank pressure is 0.5 kg / cm 2 ,...
Embodiment 2
[0015] Embodiment 2 prepares secondary bacillus megaterium seed
[0016] 1. Cultivate the first-class seeds of Bacillus megaterium (the bacteria are purchased from the strain preservation center of Beijing Institute of Microbiology, Chinese Academy of Sciences) by shaking the shaker flask. 7.8, the growth cycle is 14 hours.
[0017] 2. Place the secondary seed medium in the seed tank and carry out sterilization treatment. The sterilization temperature of the seed tank liquid is 121 ° C, the holding time is 30 minutes, and the pH value of the seed liquid after sterilization is 7.5-7.8; then the primary Seeds are inoculated into the seed tank, and the inoculation amount is 10% of the total amount (first-level seed + second-level seed medium), so that the temperature in the seed tank is controlled at 30 ° C ± 1, and the tank pressure is 0.4-0.6 kg / cm 2 , the speed of the seed tank is 180 rpm, and the ventilation volume is 7m 3 / hr, the production cycle is 12 hours.
[0018] I...
Embodiment 3
[0021] Production of compound biological fertilizer by combined solid-state fermentation of Bacillus jelly-like and Bacillus megaterium
[0022] (1) Weigh the components of the solid medium according to the formula requirements, totaling 100 kg, including 23 kg of bran, 30 kg of straw, 15 kg of fly ash, 6 kg of bean cake powder, CaCO 3 (quicklime) 0.7 kg, grass carbon 10 kg, K 2 HPO 4 0.3 kg, 3 kg of sucrose, 12 kg of loam, the straw is crushed to 20 mesh, and the rest of the components are in powder form;
[0023] All the above components are purchased from the market, K 2 HPO 4 It is a powder product.
[0024] (2) mix each component and stir evenly, send into solid fermenting tank and carry out sterilization, after solid fermenting tank is sealed, heat up to 121 ℃ and keep warm for 2 hours, cool down naturally for use;
[0025] (3) Second-level jelly-like bacillus seeds and second-level bacillus megaterium seeds in the seed tanks of embodiment 1 and embodiment 2 were r...
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