Composite bacillus preparation containing three strains, preparation method of composite bacillus preparation and application of composite bacillus preparation to ecological breeding

Abstract

The invention provides a composite bacillus preparation containing three strains, a preparation method of the composite bacillus preparation and application of the composite bacillus preparation to ecological breeding, and belongs to the technical field of microorganism and feed additives. Bacillus coagulans, butyric acid clostridia and bacillus megaterium are used as fermentative strains respectively, and large-scale, low-cost and high-density fermentation production of spores is conducted through step-by-step magnification; three live bacterium preparations are prepared by mixing bacterial sludge with the spores and dry starch; the three live bacterium preparations are matched according to the equal weight ratio, the composite bacillus preparation is obtained, and the concentration of the spores reaches more than 1*108-109 cfu / g; the composite bacillus preparation serves as a microorganism and feed additive to be added to farmed animal feed according to the mass ratio ranging from 0.02% to 0.5%. The composite bacillus preparation is simple and stable in process, low in cost, high in spore content and environment tolerance, easy to store and high in survival rate; the purposes of composition and synergism are achieved through the collaborative supplementary effects of different bacilli in the aspect of micro-ecology adjustment, growth of farmed animals is promoted, the feed utilization rate and feed rewards are increased, diarrhea is prevented and reduced, and the breeding ecological environment is improved.

Description

technical field [0001] The invention relates to a compound bacillus preparation with high live bacteria content, high spore content and long shelf life, its production and preparation method, and its application as a microbial feed additive in animal ecological breeding, belonging to the technical field of microorganisms and feed additives. Background technique [0002] In recent years, with the popularization and application of high-density intensive artificial breeding technology, my country's breeding production scale has achieved rapid development, and it has become a major producer and trader of livestock, poultry and aquatic products in the world. However, the high-density intensive breeding method makes animal diseases appear to be high-incidence, and people have to use a large number of antibiotics and other drugs extensively, resulting in an increase in drug resistance and drug-resistant strains, destroying the normal micro-ecological balance of the animal intestines...

AI Technical Summary

Problems solved by technology

In particular, there is only one patent on the compound preparation of Bacillus megaterium, and it is only used for raising meat ducks

As we all know, microorganisms such as lactic acid bacteria, yeasts, and molds cannot form spores, resulting in poor stability of preparation products, intolerance to storage, intolerance to gastric acid, and easy inactivation in the gastrointestinal tract of animals to reduce their beneficial effects; while Bacillus can produce endogenous Spore-forming, strong environmental resistance, resistant to acid, alkali, high temperature and high pressure, not easy to inactivate during feed processing and storage, easy to colonize in animal intestines, maintain a high number of viable bacteria and give full play to its physiological effects

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