Bacillus megaterium living bacteria agent, liquid fermentation process and application thereof

A technology of Bacillus megaterium and liquid fermentation, applied in application, bacteria, food processing, etc., to achieve green research and development, meet demand, and overcome the effect of low yield

Inactive Publication Date: 2019-10-11
YANGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the liquid fermentation process of Bacillus megaterium BM1259 has not been reported

Method used

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  • Bacillus megaterium living bacteria agent, liquid fermentation process and application thereof
  • Bacillus megaterium living bacteria agent, liquid fermentation process and application thereof
  • Bacillus megaterium living bacteria agent, liquid fermentation process and application thereof

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Effect test

Embodiment 1

[0029] Embodiment 1 Identification of Bacillus megaterium BM1259 of the present invention

[0030] Primers were designed according to the DNA sequence of the obtained strain BM1259, and its 16S rRNA was amplified and sequenced. The obtained sequence was compared with the 16S rRNA of other species in the NCBI database, and the results showed that BM1259 had a high homology with B. megaterium ATCC14581, and BM1259 was identified as a species of B. megaterium ( figure 1 ).

[0031] Embodiment 2 Shake flask test: the impact of Bacillus megaterium BM1259 on the degradation of ammonia nitrogen

[0032] Take the Bacillus megaterium with the preservation number: GMCC No: 1259, inoculate it in a Erlenmeyer flask equipped with a cell rejuvenation liquid medium (beef extract peptone), and culture it at 30-34° C. and 180 rpm for 18-20 hours. According to the inoculum amount of 10%, the activated bacterial solution was inserted into the ammonia nitrogen medium with different initial conc...

Embodiment 3

[0033]Example 3 Animal Experiment: Effects of Adding Bacillus megaterium BM1259 in Diets on Production Performance, Rumen Fermentation and Biochemical Indexes of Lactating Dairy Cows

[0034] 24 Chinese Holstein dairy cows with similar milk production, lactating age and parity were selected and randomly divided into 4 groups with 6 cows in each group. d (control group), 5g / d, 10g / d and 15g / d, to study the effect of Bacillus megaterium BM1259 on the production performance, rumen fermentation and biochemical indicators of lactating dairy cows. The result is as follows:

[0035] (1) The standard milk yield, milk protein rate and milk fat rate of the experimental group supplemented with 10-15g Bacillus megaterium BM1259 were significantly higher than those of the control group. The apparent digestibility of neutral detergent fiber nutrients 15g / d group was significantly higher than that of the control group (Table 1), wherein the control group: the level of Bacillus megaterium BM...

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Abstract

The invention discloses a bacillus megaterium living bacteria agent, a liquid fermentation process and application thereof. The bacillus megaterus living bacteria agent is prepared by adding bacillusmegaterium into a culture medium and carrying out liquid fermentation, wherein the preservation number of the bacillus megaterium is GMCC No:1259, and the culture medium comprises, 0.5% of sucrose, 0.8% of corn starch, 3% of soybean cake powder, 0.8% of corn steep powder, 0.5% of peptone, 0.05% of calcium carbonate, 0.08% of dipotassium hydrogen phosphate, 0.13% of magnesium sulfate, 0.1% of sodium chloride, 0.07% of zinc sulfate and 0.01% of potassium nitrate. According to the bacillus megaterium living bacteria agent, the liquid fermentation process and the application thereof, the production efficiency of the bacillus megaterium 1259 is greatly improved, the yield is increased, and a method is simple. In dairy cow production, the addition of the bacillus megaterium BM1259 in daily ration can significantly improve the production performance of dairy cows, promote fermentation in rumens and reduce the loss of nitrogen in excreta.

Description

technical field [0001] The invention belongs to the field of microbial additive fermentation production, and in particular relates to a bacillus megaterium bacterial agent, a liquid fermentation process and its application. Background technique [0002] With the continuous improvement of the intensification of my country's livestock and poultry breeding industry and the large investment of high-nutrition feed, the environmental pollution caused by nitrogen emissions is intensifying. First, excess nitrogen is emitted into the air to form greenhouse gases (N 2 O) and malodorous gas (NH 3 ), wherein the malodorous gas not only affects the production performance of animals, but also has adverse effects on the health of the breeders. Secondly, excessive nitrogen enters the feces and urine into rivers, causing eutrophication of water bodies, and even enters surface water and groundwater in the form of nitrate nitrogen and nitrite nitrogen, threatening the safety of drinking wate...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23K50/10A23K10/12A23K10/30A23K10/38A23K10/37A23K20/26A23K20/24A23K20/22A23K10/18C12R1/11
CPCC12N1/20A23K50/10A23K10/12A23K10/30A23K10/38A23K10/37A23K20/26A23K20/24A23K20/22A23K10/18Y02P60/87
Inventor 王琳赵国琦戴怡程秀花马钱波孙盛楠林淼黄倩倩
Owner YANGZHOU UNIV
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