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Novel photosynthetic organism hydrogen generation process based on oscillation-light-filling strategy

A technology of photosynthetic bacteria and hydrogen, applied in the energy field, can solve problems such as hindering light transmission, decreasing hydrogen production efficiency, and uneven light intensity distribution

Inactive Publication Date: 2012-12-05
EAST CHINA UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The light intensity in the photobioreactor (Photobioreactor, PBR) decreases sharply with the increase of the light path, and the light intensity distribution is very uneven; at the same time, some substrates tend to be colored during the metabolic process, which further hinders the transmission of light. Pass
On the other hand, with the metabolic growth of photosynthetic bacteria and the increase of biomass, the light transmittance in PBR will decrease, so the efficiency of hydrogen production which relies on light energy will decrease significantly

Method used

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  • Novel photosynthetic organism hydrogen generation process based on oscillation-light-filling strategy
  • Novel photosynthetic organism hydrogen generation process based on oscillation-light-filling strategy
  • Novel photosynthetic organism hydrogen generation process based on oscillation-light-filling strategy

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0107] 1Materials and methods

[0108] 1.1 Strains and culture conditions

[0109] Strain: Rhodobacter sphaeroides ZX-5 (public number CN 101168729A purchased from Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences);

[0110] Seed culture: 1ml Rhodobacter sphaeroides ZX-5 glycerol tube bacteria solution was inoculated into a 250ml Erlenmeyer flask containing 100ml RCVBN seed medium, initial pH=7±0.5, 30±1℃, 200rpm, aerobic culture without light, about 17h inoculation In hydrogen production medium;

[0111] Hydrogen production culture: 1ml seed solution is inoculated in 38ml photosynthetic test tube (sealed with rubber stopper) containing 34ml RCVBN hydrogen production medium (where the nitrogen source is replaced by sodium L-glutamate), initial pH=7±0.5, 30 ±1℃, light anaerobic culture, and use 50ml sterile syringe to collect hydrogen.

[0112] 1.2 Determination of light attenuation kinetics

[0113] Take a series of photosynthetic bacteria culture solutions of di...

Embodiment 2

[0193] Using Rhodobacter capsulatus to produce hydrogen by shaking fermentation

[0194] Example 1 was repeated, and the main difference was that: Rhodobacter capsulatus (Shi X-Y et al., 2005) as given in Table 6 was used to replace Rhodobactersphaeroides ZX-5.

[0195] The results show that when the carbon source is 30mM acetic acid, when I 0 Is 4000lux, I 1 In the case of 6000 lux, although the total substrate conversion rate is not greatly improved (about 10-20%), the hydrogen production rate is greatly increased, the maximum hydrogen production rate is increased by 80%, and the average hydrogen production rate is also increased by 70%. The range shortens the production cycle.

Embodiment 3

[0197] The influence of other factors (such as the amount of inoculation) on the substrate conversion rate

[0198] In this example, by changing the inoculum amount, the effect on the substrate conversion rate and hydrogen production rate during static culture or non-static culture (shaking fermentation) was observed.

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Abstract

The invention provides a novel photosynthetic organism hydrogen generation process based on the oscillation-light-filling strategy. Specifically, the invention provides a hydrogen generation method utilizing photosynthetic bacteria. The method comprises the following steps: (1) culturing photosynthetic bacteria capable of generating hydrogen under the nonstatic state and the irradiation of initial incident light with the light intensity I1, wherein the light intensity I1 of the initial incident light satisfies the following conditions: (i) I1>I0, I0 is the optimal light intensity of the initial incident light during static cultivation; (ii) under the light intensity I1, the actual light intensity I1' in at least 70% of area (or volume) of the culture system is larger than or equal to the actual light intensity I0' in the same area (or volume) during static cultivation; and (2) separating the generated hydrogen from the culture system. By using the method of the invention, hydrogen generation efficiency of photosynthetic bacteria can be effectively improved, hydrogen generation period can be shortened and high substrate conversion efficiency can be maintained.

Description

Technical field [0001] The invention relates to the field of energy, and more specifically to a novel photosynthetic biological hydrogen production process based on an oscillation-filling light strategy. Background technique [0002] With the continuous progress of society and the continuous improvement of the degree of industrialization, mankind's demand for energy is increasing day by day, and limited fossil energy can no longer meet the energy demand of all countries in the world. Energy shortages and environmental pollution are problems facing mankind in the future. The search for renewable clean energy has become the focus of attention in the energy community and related fields worldwide. At present, 80% of the energy needed in the world comes from fossil fuels, but its reserves are limited and tend to be exhausted. CO is produced when fossil fuels are burned x , SO x , NO x , CH x , Smog, dust, tar and other organic compounds, causing serious environmental pollution and ch...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P3/00C12R1/01
Inventor 王永红张明李旭张嗣良张波储炬庄英萍鲍杰
Owner EAST CHINA UNIV OF SCI & TECH
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