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Chinese beimao spore liquid culture fermentationi technology

A technology of Chinese hairy spores and liquid strains, applied in the direction of fungi, etc., can solve the problems of different production processes, liquid strain preservation problems not mentioned, and different medium formulations, etc., to achieve light color and shorten activation time , the effect of small footprint

Inactive Publication Date: 2004-03-03
安徽林苑虫草研究所
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  • Abstract
  • Description
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AI Technical Summary

Problems solved by technology

The main reasons are: (1) the strains used are not the same strain; (2) the medium formula is different; (3) the production process is different; (4) the preservation of liquid strains is not mentioned

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0039] The Chinese Mortierella liquid strain fermentation process method includes the following production steps:

[0040] A. The acquisition of strains

[0041] RCEF0273 strain (Hirsutella sinensis Liu, Guo, Yu & Zeng) was isolated from the subunit of Cordyceps sinensis (Berk) Sacc. collected in Qinghai.

[0042] B. Strain culture in a solid triangular flask:

[0043] Inoculate the isolated and purified strains into the culture medium of the Erlenmeyer flask, put them into an incubator at 19°C, and cultivate them for 20 days. The weight ratio of raw materials is: 200g potato juice, 20g glucose, 10g maltose, 20ml wort, 10g peptone, 10g yeast powder, MgSO 4 1.5g, KH 2 PO 4 3g, 0.4g ammonium citrate, 20g agar, add water to 1L, pH6.5.

[0044] C. Liquid shake flask seed culture:

[0045] In a 500ml conical flask, put 200ml of liquid seed culture medium into it, and sterilize it under high pressure at 1.5pa / kg for 30 minutes. When it is cooled to room temperature, inoculat...

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Abstract

A liquid fermentation process for the Chinese pilous spore includes such steps as getting bacterial spawn, Erlenmeyer flask culture of bacterial spawns, liquid seed culture, and liquid fermenting. Its advantags are high successful rate, low cost and no environmental pollution. The resultant mycelia can be used for medical or health-care product. The fermented liquid can be used as oral liquir or beverage.

Description

technical field [0001] The present invention relates to the fermentation process of the anamorphic Mortierella sinensis liquid strain of Cordyceps sinensis, specifically the anamorphic propagation of the Mortierella sinensis (Hirsutella sinensis Liu, Guo, Yu & Zeng) RCEF0273 strain isolated from Cordyceps sinensis The liquid strain fermentation process method and preservation method of the invention. Background technique [0002] The medicinal value of Cordyceps sinensis (Berk.) Sacc. has long been recorded in ancient my country. It is a traditional precious Chinese medicine. It is as famous as ginseng and velvet antler. It is one of the three famous tonics. Based on the role of Cordyceps sinensis in medicine and health care, and the excessive collection of natural resources, which are on the verge of exhaustion, the existing production cannot meet the demand, and the price has also doubled. In the 1970s, it was only sold for tens of yuan per 500 grams, but the current marke...

Claims

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Application Information

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IPC IPC(8): C12N1/14
Inventor 李春如樊美珍李增智黄勃
Owner 安徽林苑虫草研究所
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