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71 results about "Induction Phase" patented technology

Multiple-variable dose regimen for treating TNFalpha-related disorders

Multiple-variable dose methods for treating TNFα-related disorders, including Crohn's disease and psoriasis, comprising administering TNFα inhibitors, including TNFα antibodies, are described. Multiple-variable dose methods include administration of a TNF-inhibitor in an induction or loading phase followed by administration of the agent in a maintenance or treatment phase, wherein the TNF-inhibitor is administered in a higher dosage during the induction phase.
Owner:ABBVIE BIOTECHNOLOGY LTD

Fast calibration method of single-phase electric energy meter

The invention relates to a fast calibration method of a single-phase electric energy meter. According to the fast calibration method, the gain initial value of a power gain correction register of a metering chip of a calibrated meter is preset so as to be fast positioned to a target error value, only a 100%Ib-point 0.5-induction error point is debugged; apparent power deviation and active power deviation are simultaneously obtained at the error point; 100%Ib-point 1.0-resistance gain correction is performed through utilizing the apparent power deviation; 100%Ib-point 0.5-induction phase correction is performed through utilizing the active power deviation; and a 100%Ib-point 0.5-induction error value which has been obtained through calculation is directly utilized to perform 5%Ib-point 1.0-resistance small signal correction, and therefore, too long debugging time of small signals can be avoided. The fast calibration method has the advantages of fast error target value positioning, high meter calibration efficiency and significantly-improved accuracy.
Owner:NINGBO SANXING MEDICAL & ELECTRIC CO LTD

Tissue culture and rapid propagation method of Jiuhua Polygonatum sibiricum

The invention provides a tissue culture and rapid propagation method of Jiuhua Polygonatum sibiricum. The tissue culture and rapid propagation method comprises the following step of: allocating and optimizing culture medium formulas and culture conditions of all culture phases by taking a Polygonatum sibiricum root-like stem as an explant through adventitious buds induction phase, callus propagation phase, root-taking phase and acclimatization and transplanting phase, so as to form a tissue culture technology which has a propagation speed of 106 times each year. The tissue culture and rapid propagation method realizes the tissue culture and the rapid and stable propagation of the Jiuhua Polygonatum sibiricum and can effectively solve the problem of high-quality seedling supply of the Jiuhua Polygonatum sibiricum.
Owner:QINGYANG JIUHUA RHIZOMA POLYGONATI INDAL ASSOC

Intracranial pressure monitoring method based on magnetic induction phase difference change

InactiveCN103598883AImproving medical references for providing informationDiagnostic recording/measuringSensorsPhase differenceInduction Phase
An intracranial pressure monitoring method based on magnetic induction phase difference change comprises the following steps of (1) arranging an exciting coil on the periphery of the brain and generating a drive signal with a frequency of 10MHz and power of 1w, wherein the drive signal transmits through the brain; (2) arranging a receiving coil on the periphery of the brain, wherein a superposition sensing signal of a signal of an induced magnetic field produced by the tested brain and a sensing signal of the exciting coil are picked up by the receiving coil; (3) conducting difference calculations on a phase of the drive signal and a phase of the superposition sensing signal of the receiving coil picked up in the step (2) through a computer to obtain a variable curve of magnetic sensing phase difference along with time; (4) obtaining a intracranial pressure value corresponding to a point on a changing curve of the magnetic sensing phase difference along with the time and a changing curve of intracranial pressure along with the time in step (2). By means of the monitoring method, the intracranial pressure can be monitored.
Owner:ARMY MEDICAL UNIV

Method for Monitoring the Depth of Anesthesia

A method for monitoring the depth of anesthesia is provided for detecting the conscious state of one being anesthetized in the recovery phase or induction phase of anesthesia course in order to facilitate an anesthesiologist to predict exactly the dosage of an anesthetic required. At first, an original electroencephalogram (EEG) is taken from one being tested. Then, the original electroencephalogram is analyzed by approximate entropy to obtain its approximate entropy value. Next, the approximate entropy value is multiplied by 1000 / 17, and the corrected value is assumed as the predicted value of depth of anesthesia. The predicted value of depth of anesthesia represents degree of the conscious state or the depth of anesthesia for the one being tested. The higher the predicted depth of anesthesia value, the more conscious the one being tested is, i.e., in a shallower depth of anesthesia. On the other hand, the lower the predicted depth of anesthesia value, the less conscious the one being tested is, i.e., in a deeper depth of anesthesia.
Owner:YUAN ZE UNIV

Composite forward osmosis membrane and preparation method thereof

The present invention relates to a composite forward osmosis membrane and a preparation method thereof. The composite forward osmosis membrane comprises an ion exchange resin doped polymer support layer and a polyamide active layer compounded on the polymer support layer surface, wherein a mass ratio of the ion exchange resin to the polymer in the polymer support layer is (0.1-25):(5-40). The preparation method comprises: doping ion exchange resin micro-particles in a polymer solution, preparing a composite forward osmosis membrane support layer containing the ion exchange resin by using a non-solvent induction phase separation method, and compounding an active layer adopting polyamine and poly acyl chloride on the surface of the composite forward osmosis membrane support layer containing the ion exchange resin by using an interfacial polymerization method so as to prepare the composite forward osmosis membrane, wherein the prepared composite forward osmosis membrane can be adopted as the osmosis membrane for seawater desalination and other processes. Compared with the method in the prior art, the method of the present invention has advantages of economy, high efficiency, high water flux, high salt trapping rate, and the like.
Owner:EAST CHINA UNIV OF SCI & TECH

Methods of treating eosinophilic esophagitis

The present disclosure provides methods of treating inflammation of the upper gastrointestinal tract, especially the esophagus, by administering an oral corticosteroid. In some cases, the methods include treating eosinophilic esophagitis (EoE) by administering an oral corticosteroid in an induction phase and a maintenance phase to improve peak eosinophilic counts and symptoms. In embodiments, the methods include treating EoE by administering the oral corticosteroid at nighttime and / or while the patient is lying down.
Owner:ELLODI PHARM LP

Process for plasmid DNA fermentation

ActiveUS7943377B2Improve plasmid DNA production yieldImprove plasmid DNA qualityGenetic therapy composition manufactureTissue cultureBatch fermentationPlasmid dna
Improvements in plasmid DNA production technology are needed to insure the economic feasibility of future DNA vaccines and DNA therapeutics. General methods are described, by means of which it is possible to dramatically increase plasmid DNA productivity in a fermentor. These processes feature Fed-batch fermentation strategies, combined with novel growth and induction phase temperature shifts.
Owner:ALDEVRON LLC

Three-phase unequal-turns fractional slot concentrated winding

The invention relates to a three-phase unequal-turns fractional slot concentrated winding, wherein each phase of winding is formed by serially connecting 12 coils with pitch of 1; each coil of four coils in the 12 coils has the turns of n, wherein n is a natural number, and each coil of the rest 8 coils has the turns of 2n; the turns ratio of two kinds of unequal-turns coils is 2:1; three phases of windings are in difference of 1200 electric angles and are of three-phase symmetrical windings; the slot fullness of each slot is balanced; a stator iron core is inclined to 1 / 7 slot pitch; and thethree phases of windings can be in r connection or Y connection. The unequal-turns fractional slot concentrated winding not only preserves the advantages, such as coil pitch of 1, capability of beingdirectly wound on one tooth and short winding end part of a concentrated winding with slot number q=1 / 2 of each phase and each pole, but also overcomes the serious defects that the q=1 / 2 equal-turns concentrated winding has the potential harmonic content up to 27.3 percent in the induction phase and poor performance, and a multipole permanent magnet servo motor cannot be used in a servo system with strict requirements on position precision. The three-phase unequal-turns fractional slot concentrated windings has the potential harmonic content less than 2 percent in the induction phase, reachesan ideal sinusoidal wave state, ensures that the permanent magnet servo motor reaches high performance, can be used for the fields, such as numerical control laths having strict requirement on position precision, and the like and has the advantages of traditional process, easiness in implementation and excellent performance, thereby having an actual popularization meaning.
Owner:TELLHOW SCI TECH CO LTD

Method for setting initial green light time based on pedestrian and bicycle group

InactiveCN101819719ASure easySignal timing results are realisticControlling traffic signalsInduction PhaseGreen-light
The invention discloses a method for setting an initial green light time based on a pedestrian and bicycle group, which relates to a semi-actuated signal timing algorithm of a two phase signalized intersection and is used for determining an initial green light time meeting an induction phase of queuing left-turning vehicles at a signalized intersection under semi-actuated control. The method mainly comprises the following steps of: observing a critical gap and an average acceptable gap provided by the pedestrian and bicycle group which is in conflict with left-turning vehicles; determining the relation between average acceptable gap time and the number of traversable left-turning vehicles; determining the arrival rate of the left-turning vehicles arriving during red light period and the initial green light period; and determining the relation between left-turning vehicle arrival and dispersive balance. The invention improves the reasonability of the setting of actuated control phase initial green light time, can provide sufficient passing time for the left-turning vehicles to pass through a crossing and improve the control efficiency of the whole crossing.
Owner:BEIJING UNIV OF TECH

Micro-potato production method by mist culture method

The invention discloses a micro-potato production method by a mist culture method. By the micro-potato production method, micro-potatoes are cultured by the aid of mist spraying technique, the whole culture process is divided into a growth phase, an induction phase and a tuberization phase by combining growth characteristics of micro-tubers, and three different nutrient solutions including growth phase nutrient solution, induction phase nutrient solution and tuberization phase nutrient solution are used, so that better growth of the micro-potatoes is promoted. The micro-potato production method has the advantages that micro-potato growth period is shortened, the tuberization quantity is increased, and single micro-potato weight is increased.
Owner:SHUICHENG DASHAN SEED IND CO LTD

Method for high-density fermentation of pichia pastoris of insulin precurosor protein

The invention relates to the field of microorganisms and discloses a method for high-density fermentation of pichia pastoris of insulin precurosor protein. The method comprises the steps of expanding culture of strain, fermentation culture and thallus collection. Fermentation culture is divided into a batch growth phase, a glycerinum replenishment phase and a methyl alcohol induction phase. By controlling parameters including age, replenishing rate and pH value of strain during fermentation, the concentration of glycosylation 2 can be effectively reduced by 60+ / -8%, and the unit yield of insulin precurosor protein can be increased by 25% or above. Furthermore, the method has the advantages of being simple, easy to operate, low in cost and the like.
Owner:YICHANG HEC CHANGJIANG PHARMA CO LTD

Method for monitoring the depth of anesthesia

A method for monitoring the depth of anesthesia is provided for detecting the conscious state of one being anesthetized in the recovery phase or induction phase of anesthesia course in order to facilitate an anesthesiologist to predict exactly the dosage of an anesthetic required. At first, an original electroencephalogram (EEG) is taken from one being tested. Then, the original electroencephalogram is analyzed by approximate entropy to obtain its approximate entropy value. Next, the approximate entropy value is multiplied by 1000 / 17, and the corrected value is assumed as the predicted value of depth of anesthesia. The predicted value of depth of anesthesia represents degree of the conscious state or the depth of anesthesia for the one being tested. The higher the predicted depth of anesthesia value, the more conscious the one being tested is, i.e., in a shallower depth of anesthesia. On the other hand, the lower the predicted depth of anesthesia value, the less conscious the one being tested is, i.e., in a deeper depth of anesthesia.
Owner:YUAN ZE UNIV

Silicone free Anti-foaming process and controlled foaming process for petroleum coking

The current invention provides an improved petroleum coking process wherein the risk of silicone poisoning of units downstream of the coke drums is eliminated. The method of the current invention controls the foam layer within the coke drum by injection of a non-silicone anti-foam agent, preferably a tire oil. In another embodiment, the current invention provides a controlled foaming method which increases the liquid production from the coke drum. In the controlled foaming method, an anti-foam agent is cyclically injected into the drum early in the coke drum fill cycle. The cyclic injections manage the foam layer without inducing the induction phase in the coke feedstock, thereby increasing the total volume of feedstock received by the coke drum.
Owner:UNIVERSITY OF TULSA

Somatic embryogenesis and plant regeneration method for Picea asperata Mast

The invention relates to a somatic embryogenesis and plant regeneration method for Picea asperata Mast. The method comprises the following steps: collecting freely-pollinated clonal cones of the Picea asperata Mast, and isolating immature zygotic embryos for somatic embryogenesis. The somatic embryogenesis comprises four phases: an embryonic callus induction phase, an embryonic callus proliferation phase, a somatic embryo mature phase and a somatic embryo germination phase. Compared with the conventional international research on the same or similar tree species, the frequency of embryonic callus induction is greatly increased, and the differentiation capacity and germination capacity of somatic embryos are also greatly increased. Meanwhile, a long-time keeping and proliferation method for embryonic callus is obtained, thus greatly reducing the risk of embryonic loss.
Owner:INST OF FORESTRY CHINESE ACAD OF FORESTRY

Array substrate, related current detection method, display panel and display device

The invention discloses an array substrate, a related current detection method, a display panel and a display device. A part, exposed by an open hole, in a first power line serves as an induction electrode; when touch control or fingerprint identification is carried out, a coupling capacitor is formed between the finger and the induction electrode; the size of the coupling capacitor is changed along with the distance between the finger and the sensing electrode. A pixel circuit electrically connected with an induction electrode is set to comprise a first switch transistor; The first switchingtransistor is configured to be in an induction phase. Under the control of a signal of the first control signal end, a signal of the induction electrode is transmitted to the driving transistor, the driving transistor outputs current to the current detection end, the current is related to the size of the coupling capacitor, and therefore the functions of touch control and fingerprint recognition can be achieved by detecting the current change of the current detection end. In addition, the scheme of the invention is simple, no complex Mask process needs to be added, the cost is low, and the current during touch control or fingerprint identification is measured.
Owner:BOE TECH GRP CO LTD +1

Inductive arterial road coordination control method based on electronic tag

InactiveCN105303849AOvercome sensory control failureEffective use of green light timeControlling traffic signalsEngineeringGreen-light
The invention relates to an inductive arterial road coordination control method based on an electronic tag. An induction phase reads the number of vehicles with electronic license plates in a road section through an RFID reader which is arranged at an intersection. The density of the vehicles in the road section is used as a control index to carry out induction control on the induction phase, so that the inductive coordination control induction phase overcomes failed induction control when traffic flow is supersaturated. The inductive arterial road coordination control coordination phase operates in a fixed timing scheme mode, participates in intersection coordination. A non-coordinated phase operates in an induction control mode to adapt to the randomness of traffic flow reaching under traffic control. Main road green wave control is realized. The green light time of secondary road traffic flow is effectively used. The average delay and the average number of stops of vehicles at the intersection can be effectively reduced.
Owner:SHANGHAI INST OF TECH

Method for preparing hydrophilic charged composite ultrafiltration membrane

The invention relates to a method for preparing a hydrophilic charged composite ultrafiltration membrane. The method comprises together dissolving acrylamide (AM), acrylic acid (AA), 2-acrylamide-2-methylpropanesulfonic acid (AMPS) and a high-molecular membrane-preparing materials in a membrane solution, adding an initiator into the membrane-forming solution to initiate free radical polymerization and generate a high-molecular copolymer additive in the membrane-forming solution. The high-molecular copolymer additive is strong in hydrophilia, is negatively charged, and is easy to disperse and dissolve in the membrane-forming solution. The membrane-forming solution containing the high-molecular copolymer additive is used to prepare the composite ultrafiltration membrane by employing a non-solvent induction phase separation process, so that the high-molecular copolymer additive is embedded into the membrane structure, and introduced various hydrophilic and charged functions are not easy to lose. According to the method for preparing the hydrophilic charged composite ultrafiltration membrane, the high-molecular copolymer additive molecule, which is strong in hydrophilia and negatively charged, is embedded into the membrane, and helps to effectively improve the hydrophilia, the membrane flux and the antipollution capability of the ultrafiltration membrane, and the ultrafiltration membrane is lasting and stable in performances.
Owner:CHINA PETROLEUM & CHEM CORP +1

Methods for expression and purification of immunotoxins

In one aspect the present invention relates to a method of expressing an immunotoxin in Pichia pastoris strain mutated to toxin resistance comprising a) growing the Pichia pastoris in a growth medium comprising an enzymatic digest of protein and yeast extract and maintaing a dissolved oxygen concentration at 40% and above; and b) performing methanol induction with a limited methanol feed of 0.5-0.75 ml / min / 10 L of initial volume during induction along with a continuous inusion of yeast extract at a temperature below 17.5° C., antifoaming agent supplied up to 0.07%, agitation reduced to 400 RPM, and the induction phase extended out to 163 h. In another aspect, the present invention relates to a method of purifying a nonglycosylated immunotoxin comprising a) loading a solution containing the nonglycosylated immunotoxin onto a hydrophobic interaction column; b) obtaining a first non-glycosylated immunotoxin containing eluant from the hydrophobic interaction column; c) loading the non-glycosylated immunotoxin containing eluant from step (b) onto an anion exchange column; d) obtaining a second non-glycosylated immunotoxin containing eluant from the anion exchange column by eluting the non-glycosylated immunotoxin with a sodium borate solution; e) diluting the concentration of sodium borate in the second non-glycosylated immunotoxin containing eluant from step (d) to about 50 mM or less; f) concentrating the diluted non-glycosylated immunotoxin containing eluant from step (e) over an anion exchange column; and g) obtaining a purified non-glycosylated immunotoxin from the anion exchange column.
Owner:UNITED STATES OF AMERICA

Crystalline polyaryletherketone porous membrane with adjustable pore diameter, preparation method and application of membrane

The invention discloses a crystalline polyaryletherketone porous membrane with an adjustable pore diameter, a preparation method and application of the membrane, and belongs to the technical field ofpolymer materials. A soluble polyaryletherketone precursor solution is prepared into a porous membrane by means of an immersion phase inversion method or a steam induction phase inversion method or atemplate method, then the porous membrane is crystallized through acidification and heat treatment, and therefore the crystalline polyaryletherketone porous membrane with the adjustable pore diameteris prepared. The crystallinity of the crystalline polyaryletherketone porous membrane with the adjustable pore diameter is 26-29%, the pore diameter of the membrane is within the range of 0.01-5 microns, the porosity of the membrane is 50-80%, pores are finger-shaped pores or sponge-shaped pores, and the thickness is 10-130 microns. The operation of the method is simple, the cost is low, large-scale production can be achieved, the immersion phase inversion method and the steam induction phase inversion method do not require external auxiliaries, the process cost is further reduced, and the crystalline polyaryletherketone porous membrane can be widely applied to fuel cells, super capacitors, lithium ion batteries, ultrafiltration membranes and the like.
Owner:JILIN UNIV

Multi-cylinder diesel engine with variably actuated valves

In a Diesel engine with variably actuated valves, the cam controlling each inlet valve is shaped to provoke the opening of the respective inlet valve during the engine's normal exhaust phase and thus realize exhaust gas recirculation within the engine, due to the fact that during the normal exhaust phase, part of the exhaust gas passes from the cylinder into the inlet port, from where it returns to the cylinder during the next induction phase, while part of the exhaust gas that had previously passed into the exhaust port returns to the cylinder during the induction phase due to the additional opening of the exhaust valve, in consequence of which the exhaust gas charges that return to the cylinder are subjected to further combustion in the next engine cycle.
Owner:CENT RICERCHE FIAT SCPA

Biological tissue blood flow real-time monitoring system and simulation monitoring system based on magnetic induction phase displacement

The invention discloses a biological tissue blood flow real-time monitoring system and simulation monitoring system based on magnetic induction phase displacement. The system comprises a signal source, an exciting coil unit, a receiving coil unit, a digitizer and a host computer PC. The signal source outputs two sinusoidal signals with the same frequency and phase and is respectively connected with the exciting coil unit and the digitizer, and the receiving coil unit is connected with the digitizer and is used for collecting an output signal which is sent by the exciting coil unit and is generated in the receiving coil unit after passing through a to-be-detected part; and the real-time state of the to-be-detected part is obtained by transmitting the output signals to the host computer for analysis and processing. The system provided by the invention utilizes the magnetic induction phase shift to monitor the cerebral blood flow pulsation condition, continuously and effectively monitors the change of the cerebral blood flow pulsation through the combination of the arterial blood flow dynamics theory and the magnetic induction phase shift detection principle, and has the advantages of being noninvasive, safe, non-contact, small in size and high in penetrability.
Owner:CHONGQING UNIV OF TECH

Test device

ActiveUS20180010084A1Determination of bacterial identification or antimicrobial susceptibilityEasy to determineBioreactor/fermenter combinationsImage enhancementMicroscopic observationPhacus
The invention provides a technology for promptly determining bacterial identification or an antimicrobial susceptibility testing. In the invention, first, a state where the bacteria are divided is monitored by performing microscopic observation with respect to the shape or the number of bacteria in each of wells of a culture plate for bacterial identification culture or the antimicrobial susceptibility testing. In addition, the shape, the number or the area of the bacteria are interpreted from the image obtained by the microscopic observation whether or not the bacteria proliferate at a stage from an induction phase to a logarithmic phase, and the time-dependent changes thereof are made into a graph. From the graph, it is determined whether or not the bacteria proliferate for each measurement, the determination results are displayed on the screen, and accordingly, the result of the antimicrobial susceptibility is provided every time when the measurement is performed (FIG. 12).
Owner:HITACHI HIGH-TECH CORP

Collagen phased mixing, induction and fermentation method

The invention discloses a collagen phased mixing, induction and fermentation method, which belongs to the technical field of protein engineering. The method comprises the following steps: purifying and preserving a strain, activating the strain and spreading cultivation; shaking a flask, culturing seeds in a seed tank; culturing the strain in a fermentation cylinder and acclimating the strain by methanol, carrying out an initial induction phase and a mixing increased induction phase; and post-processing a broth to obtain the collagen. The mixing increased induction mode by combining an inducer, low temperature and high power is employed, compared with induction carried out by adding the inducer at a single phase, the strain has better adaptation process to carbon source methanol, biomass accumulation can be further realized, induction intensity can be gradually enhanced, and output of target protein can be greatly increased; compared with the situation that only a temperature induction mode is used, correct folding of the target collagen can be guaranteed, massive requirement for the dissolved oxygen by the strain at an inducible expression phase can be satisfied by enhancing the power condition, expression output of the target collagen is increased, and poisoning phenomenon of the strain is avoided.
Owner:SHANDONG LUKANG PHARMA

Anti-radiation phase-change fabric and preparation method thereof

The invention discloses anti-radiation phase-change fabric and a preparation method thereof. The preparation method comprises the steps of spinning warp yarn by mixing nanometer metal shielding fiber yarn, fine sulfur cotton and flame retardant fiber, spinning weft yarn by mixing silver fiber with polyacrylonitrile fiber, and then spinning the warp yarn and the weft yarn into semi-finished fabric; padding and rolling the obtained semi-finished fabric in finishing liquor, and obtaining finished fabric by drying and cooling. Polyethylene glycol is an organic polymer phase-change material and has higher crystallinity degree and higher phase-change latent heat, the induction phase-change temperature of the polyethylene glycol is exactly in a range that people feel comfortable, and thus the obtained clothes fabric has good anti-radiation performance, good anti-bacterial performance, good anti-acarid performance and flame retardant performance.
Owner:EASTERN LIAONING UNIV

Three-phase unequal-turns fractional slot concentrated winding

The invention relates to a three-phase unequal-turns fractional slot concentrated winding, wherein each phase of winding is formed by serially connecting 12 coils with pitch of 1; each coil of four coils in the 12 coils has the turns of n, wherein n is a natural number, and each coil of the rest 8 coils has the turns of 2n; the turns ratio of two kinds of unequal-turns coils is 2:1; three phases of windings are in difference of 1200 electric angles and are of three-phase symmetrical windings; the slot fullness of each slot is balanced; a stator iron core is inclined to 1 / 7 slot pitch; and thethree phases of windings can be in r connection or Y connection. The unequal-turns fractional slot concentrated winding not only preserves the advantages, such as coil pitch of 1, capability of beingdirectly wound on one tooth and short winding end part of a concentrated winding with slot number q=1 / 2 of each phase and each pole, but also overcomes the serious defects that the q=1 / 2 equal-turns concentrated winding has the potential harmonic content up to 27.3 percent in the induction phase and poor performance, and a multipole permanent magnet servo motor cannot be used in a servo system with strict requirements on position precision. The three-phase unequal-turns fractional slot concentrated windings has the potential harmonic content less than 2 percent in the induction phase, reachesan ideal sinusoidal wave state, ensures that the permanent magnet servo motor reaches high performance, can be used for the fields, such as numerical control laths having strict requirement on position precision, and the like and has the advantages of traditional process, easiness in implementation and excellent performance, thereby having an actual popularization meaning.
Owner:TELLHOW SCI TECH CO LTD

Method for increasing output of alkaline pectinase produced by fermentation by adding sorbitol

InactiveCN101525604AIncrease productionDoes not inhibit enzyme productionMicroorganism based processesEnzymesPectinasePichia pastoris
The invention discloses a method for increasing the output of alkaline pectinase produced by fermentation by adding sorbitol, relating to the technical field of application of the carbon source adding policy in the optimization of fermentation. In the method, recombinant Pichia pastoris GS115 is adopted as fermentation strains, and sorbitol is added into the fermentation liquid at a constant speed or accelerated speed during fermentation induction phase to improve the activity of cells, weaken the toxicity of methanol, and increase the output of the alkaline pectinase. The lower adding amount of sorbitol can not inhibit the enzyme production effect of methanol to the strains, on the other hand, the toxicity to the strains from the methanol can be reduced to a certain extent, and the efficiency of enzyme production is further increased. When the sorbitol is added at a varying speed (0.9g / h-7.2g / h), the activity of enzyme is increased from 930U / ml to 1541U / ml. The invention is simple and effective in process, capable of being applied in the industrial production of pectinase in the future, and instructive to the optimization of fermentation of other Pichia pastoris.
Owner:JIANGNAN UNIV

A scale inhibiting method of extraction apparatus in the wet phosphoric acid purifying process

A scale inhibiting method of extraction apparatus in the wet phosphoric acid purifying process includes: adding additive agent extraction apparatus scale formation powder or calcium sulphate powder or 93% of concentrated sulfuric acid in the feed acidity of the wet phosphoric acid purify process extraction process, breaking the 'medium safe state' of the feed phosphoric acid, shorting its induction phase, speeding up calcium sulphate deposition, thereby stoping the scale formation on the extraction apparatus; the extraction apparatus scale formation powders are taken dorm form the extraction apparatus, soaking with water, washing, wherein, the scale block is collapsed into powder, drying, flour milling, sifting to obtain the power. The scale formation powder grain fineness number is 60-100 mesh, the added amount is 1-3g feed acidity per 100mL; and the added amount of the calcium sulphate is 0.2%-5.0% of the feed phosphoric acid; and the sulfuric acid dosage is 1-5 times of calcium ion content. After the feed phosphoric acid of the practical application in the inventive method can pass through decalcification preliminary treatment, the extraction apparatus scale formation phenomenon is greatly alleviated, the apparatus scavenging period is extended, thereby the apparatus production efficiency is enhanced, which is suitable for producing enterprises for purifying wet phosphoric acid.
Owner:WENGFU (GRP) CO LTD
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