67 results about "Trionyx" patented technology

The invention discloses an expanded pellet feed for the trionyx sinensis. The curing degree of the expanded pellet feed is 100 percent. The expanded pellet feed is prepared from domestic white fish meal, America white fish meal, imported eel-grade red fish meat, flour, cassava pieces, soy beans, non-transgenic soybean meal, fermented soybean meal, pregelatinization starch, milk powders, mineral premix, vitamin premix, monocalcium phosphate, calcium propionate and phosphatide oil by using a twin-screw extruding fully-curing expansion machine to firstly carry out quenching and tempering on the materials for 160 to 200 seconds under the conditions that a vapour pressure is between 0.4 and 0.5MPa, a humidity is between 19 and 23 percent, and a temperature is between 85 and 90 DEG C, and then carry out curing on obtained product under the conditions that a curing temperature is 180 DEG C, a cavity pressure is between 22 and 28atm and a curing time is between 25 and 30 seconds. Each kilogram of mineral premix comprises Mg, Na, K, Fe, Cu, Mn, Zn, Se, Co and I. The expanded pellet feed for the trionyx sinensis has the advantages of reasonable configuration of various elements required by nutrition and growth, complete curing, resistance to cause damage to all nutritional factors, low cost, higher stability in water and higher digestibility.

The invention discloses a method for integrated culture of rice-finless eel-crayfishp-trionyx sinensis. The method comprises the following steps: placing myriophyllum aquaticum in a ring-shaped ditchof a paddy field; putting crayfish seedlings in April or August, putting trionyx sinensis seedlings and cipangopaludina cahayensis in May, and putting finless eel seedlings in late June to early July;performing feeding; and catching crayfish in early June or early October, and catching finless eels and trionyx sinensis in November to December. According to the method for integrated culture of therice-finless eel-crayfish-trionyx sinensis, ecosystem biodiversity of the paddy field is improved, cannibalism degree of the crayfish is reduced, requirement for live baits of the finless eels and the trionyx sinensis is satisfied; and the method has the advantages of higher biological diversity, easy catching, adequate supply of live baits, more stable culture yields, higher economic benefits, and the likes.

The invention discloses a medicine for treating the viral hemorrhagic disease of trionyx sinensis. The medicine comprises the following components: radix isatidis, fructus forsythia, flos lonicerae, flos chrysanthemi indici, cordate houttuynia, liquorices, andrographis paniculate, radix paeoniae rubra, blackberry lily, pine needle, sodium chloride, radix astragali, goldthread root, rheum officinale, phellodendron, radix sophorae falvescentis, garlic, rhizoma calami, fructus cnidii, dried tangerine peel, hawthorns, camphor, rhizome of cyrtomium, dandelion, herba epimedii, potassium sulphate, sodium bicarbonate, vitamin C, vitamin E and vitamin K3. The medicine for treating the viral hemorrhagic disease of trionyx sinensis disclosed by the invention restrains virus, can be used for treating the viral hemorrhagic disease of turtles with effective percent of over 95%, and the cure rate in 20 days reaches 90 percent above; and the medicine for treating the viral hemorrhagic disease of trionyx sinensis has reasonable compatibility, can resist fungi as well as remove garlic stink due to the use of dried tangerine peel, hawthorns, cordate houttuynia, flos lonicerae, flos chrysanthemi indici, phellodendron and the like; the preparation method of the medicine for treating the viral hemorrhagic disease of trionyx sinensis can improve the extraction rate of effective ingredients and reduce the loss of useful substances; and firstly mixing garlic with dried tangerine peel can remove the stink of garlic in advance.

The invention discloses microsatellite DNA markers for Trionyx sinensis, which is characterized in that an enriched library of microsatellites (CA)n, (AAC)n, (ACAG)n and (GATA)n from the Trionyx sinensis is constructed, and positive clones of microsatellite sequences are screened and sequenced to obtain 63 clones containing microsatellite repetitive sequences, thus determining 15 microsatellite markers with rich polymorphism, namely, PSW01, PSW02, PSW03, PSW04, PSW05, PSW06, PSW07, PSW08, PSW09, PSW10, PSW11, PSW12, PSW13, PSW14 and PSW15. The microsatellite DNA markers for the Trionyx sinensis of the invention can be applied to researching genetic diversity and paternity test for the Trionyx sinensis in different geographical populations, have the advantage of good repeatability, and are reliable and effective molecular markers.

The invention provides a special feed for breeding trionyx sinensis and a preparation method of the special feed. The special feed is prepared from the following raw materials in parts by weight: 19-21 parts of fish meal, 4.5-5.5 parts of barley pest powder, 9-11 parts of shrimp meal, 10-12 parts of tubifex powder, 9-11 parts of soybean cake powder, 9-11 parts of wheat feed, 9-11 parts of corn flour, 4.5-5.5 parts of animal blood, 8-10 parts of wheat flour, 1 part of fish oil, 1 part of corn oil, 2.5-3.5 parts of bone meal, 0.3 part of lysine, 0.2 part of methionine, 0.5 part of salt, 2 parts of multivitamins, 1.8-2.2 parts of yeast extract powder, 0.5 part of probiotics and 0.5 part of mineral substances. The feed is scientific and reasonable in nutrient substances, and good in palatability, digestive absorption is facilitated, and the immunity of the trionyx sinensis is improved. Compared with the conventional feed, the yield of a control group of the product is improved by 4.9-6.5%, and the cost is reduced by 4.7-5.3%. The feed is simple in preparation technology, easy to operate, stable in product quality, and small in equipment investment, and has good economic benefits, social benefits and ecological benefits.

The invention provides a tortoise or trionyx sinensis spawning pond, which comprises a pond body, wherein the pond body is provided with a slope, a spawning table and a spawning groove, wherein the spawning table is provided with an installation groove; the spawning groove is arranged in the installation groove; the slope is arranged in the middle of a pond bottom; one end of the spawning table is connected with the slope; the other end of the spawning table is connected onto the pond wall; the spawning groove comprises a base plate and a three-face coaming arranged on the base plate; the spawning groove is provided with a filter screen layer and spawning sand covering the spawning groove; so that external influence on a tortoise or trionyx sinensis spawning process is low, the spawning sand is easy to clear, and the egg masses are easy to collect; after being taken out from the spawning groove, the egg masses are placed into cultivation water; and the spawning pond can be used as a tortoise or trionyx sinensis cultivation pond. The tortoise or trionyx sinensis spawning pond is simple in structure and is easy to realize.

The invention relates to a bionic mixed culture method of Trionyx sinensis and full-male Pseudobagrus fulvidraco. The method comprises the following steps of: fish pond selection and disinfection, fish seed and turtle seed putting, fish seed and turtle feed feeding, water quality regulation and alternate harvesting and putting. According to the invention, the traditional situation that the mixed culture of four household fish and turtle is replaced, full-male Pseudobagrus fulvidraco with high growth speed is selected, the culture density and specification are reasonably determined, and the technical operation instruction of culture is established, thus the problem that the comparison benefit decreases when turtle is cultured in the pond is solved, the culture risk is reduced, the water utilization factor of the pond is simultaneously increased, and high-yield, high-efficiency, ecological and healthy mixed culture of Trionyx sinensis and full-male Pseudobagrus fulvidraco is realized.

The invention discloses a compound pellet feed with low fish meal dosage for trionyx sinensis juveniles and a preparation method thereof, and belongs to the field of feeds. The feed is prepared from the following raw materials in percentage by weight: 35-40 percent of fish meal, 10-15 percent of fermented soybean meal, 10-15 percent of soybean protein concentrate, 2-3 percent of corn gluten meal, 20-22 percent of gelatinized starch, 6-12 percent of compound animal-derived nutritional attractant, 1.5-2.0 percent of mixed fat, 0.1-0.3 percent of coated methionine, 0.1-0.3 percent of choline chloride, 0.8-1.2 percent of monocalcium phosphate, 0.3-0.5 percent of vitamin premix, 0.3-0.5 percent of mineral premix and 0.01-0.02 percent of phytase. The feed is comprehensive and balanced in nutrition, and can meet the nutritional requirement for the trionyx sinensis juveniles. Compared with current trionyx sinensis juvenile commercial feeds sold in the market, the compound pellet feed has the advantages that the dosage of the fish meal is reduced, the production cost is greatly reduced, the feeding effect is good, and the feed coefficient is low.

The invention belongs to the field of Chinese medicinal preparations, in particular to a Chinese medicinal plaster for treating arthritis, which is prepared from the following Chinese medicinal raw materials: blackone chicken, live trionyx, cassia twig, Chinese ephedra, common bletilla tuber, stiff silkworm, divaricate saposhnikovia root, notopterygium root, dahurian angelica root, double-teeth pubescent angelica root, twotooth achyranthes, clematis root, large-leaved gentian, siberian cocklour fruit, erythrina bark, mulberry twig, Chinese atractylodes, szechuan lovage rhizome, frankincense, myrrh, safflower, suberect spatholobus stem, garden balsam stem, costus root, cortex eucommiae, centipede, towel gourd vegetable sponge, prepared rehmannia root, slenderstyle acanthopanax bark, scallion root, Chinese angelica, common monkshood mother root, kusnezoff monkshood root, minium, pepper root, silkworm dropping, red paeony root and amur corktree bark. The Chinese medicinal plaster has thecharacteristics of quick response, low cost, freeness from toxic and side effects, difficulty in relapsing and the like.

The invention discloses a trionyx feed, comprising the following raw materials: 35-50 parts of imported fish meal, 18-22 parts of starch, 6-10 parts of soy protein, 15-20 parts of soya bean meal, 1-3 parts of fish oil, 1-2 parts of monocalcium phosphate, 1-3 parts of adhesive, 2-5 parts of beer yeast, 0.5-2 parts of multi-vitamin, 0.1-1 part of sodium salt and 0.5-2 parts of mineral substance in parts by weight. The antibiotic-free trionyx feed of the invention has comprehensive nutritional ingredients and strong palatability and can take the effect of preventing dysplasia and anorexia of trionyx.

The invention relates to a traditional Chinese medicinal composition capable of enhancing immunity. The traditional Chinese medicinal composition is prepared from the following traditional Chinese medicinal raw materials in parts by weight: 24-48 parts of herba epimedii, 6-10 parts of ginseng, 24-48 parts of radix astragali preparata, 16-32 parts of wine ligustrum lucidum ait, 4-8 parts of turtle powder, 8-16 parts of trionyx sinensis powder, 16-32 parts of fried atractylodes macrocephala and 8-16 parts of pericarpium citri reticulatae. A traditional Chinese medicinal capsule production technology disclosed by the invention is simple; the production period is shortened; the production efficiency is improved; energy and time are saved; therefore, the production cost of a product can be reduced, and the traditional Chinese medicinal composition is suitable for industrial large-scale production.

The invention discloses a feed capable of preventing and curing diseases of young trionyx sinensis and a preparation method thereof. The feed comprises the following raw materials: pork powder, shrimp meal, purple common perilla seed cake, sorghum, wheat bran, vinegar dregs, molasses, grape pomace, peach gum, tomatoes, pineapples, satsuma mandarin, soy sauce powder, onion powder, acetate ester starch, Chinese white cabbages, water spinach, mung bean sprouts, leek flowers, radix isatidis, citrus reticulata blanco peels, coptis roots, drying rehmannia roots, Chinese yams and rhubarb. The feed can be used for solving the problems of shortage of nutrition, rough preparation, poor palatability, low growth speed of the young trionyx sinensis, poor physique, easiness in illness formation, low survival rate and the like of the conventional feed for the young trionyx sinensis, the feed is rich in nutrition, fine in taste and faint scent in mouthfeel, can promote the growth and weight increment of infant terrapin and reduce the growth time, is capable of preventing and curing diseases and reducing the infection possibility of bacteria and parasite to the young trionyx sinensis, and has the advantages that the young trionyx sinensis has strong immunity, cannot get ill, and is high in survival rate, the economical benefits of the cultivation of the young trionyx sinensis can be greatly increased, and the promotion value is extremely high.

The present invention discloses a temperature and humidity control method of trionyx sinensis incubation room. The temperature and humidity control method comprises the following control steps: (1) providing a heating apparatus, a humidifying apparatus and a ventilation apparatus in the incubation room, and providing a water sprayiing apparatus at the roof of the incubation room; and (2) by virtue of a signal collected by the temperature sensor and humidity sensor provided in the incubation room, controlling the temperature of the incubation room at 28-30 DEG C and the humidity at 78%-85% by controlling the heating apparatus, the humidifying apparatus, the ventilation apparatus and the water spraying apparatus. According to the temperature and humidity control method of trionyx sinensis incubation room disclosed by the invention, after trionyx sinensis eggs in a trionyx sinensis pond are collected in a unified mode, the method is utilized to perform incubation; and the method has characteristics of a high incubation rate and short incubation time.

The invention discloses a Chinese medicinal composition for treating hepatofibrosis, which is mainly prepared from the following main raw materials: 10-20g of trionyx sinensis shell, 10-20g of tortoise shell, 9-15g of curcuma zedoary, 12-15g of rhizoma sparganii, 18-25g of black sesame seeds, 15-18g of glossy privet fruit and 30g of dry loach powder. The following raw materials of the Chinese medicinal composition can be added selectively to treat the I-type symptoms of hepatofibrosis: 6-12g of fructus aurantii, 10g of coked malt, 10g of coked hawthorn, 10g of coked medicated leaven, and in addition, 12-18g of oriental wormwood and 10-15g of cape jasmine can be added to treat internal dampness and heat and jaundice. The following raw materials of the Chinese medicinal composition can be added selectively to treat the II-type symptoms of hepatofibrosis: 20-30g of dandelion, 6-9g of pseudo-ginseng, 6-9g of red peony root, 8-12g of curcuma aromatica, and in addition, 6-9g of hairyvein agrimony and 9-12g of eclipta alba can be added to treat hemorrhage. The following raw materials of the Chinese medicinal composition can be added selectively to treat the III-type symptoms of hepatofibrosis: 12-18g of codonopsis pilosula, 8-10g of dogwood and 9-12g of caulis spatholobi. 9-15g of subprostrate sophora and 1.5g of cordyceps sinensis can be added and decocted together with the raw materials to treat the positive marker of hepatitis B virus. The Chinese medicinal composition has a significant effect on 502 patients (69.05%) of the total 727 patients with the hepatofibrosis, is effective to 189 patients (26.00%), and is invalid to 36 patients (4.95%), thereby having the total effective rate of 95.05%.

The invention discloses a powdery formula feed for trionyx sinensis juveniles special for greenhouse breeding mode. The formula feed comprises the following raw materials: fish meal, chicken meal, maggot powder, seaweed glue, pre-gelatinized starch, fermented soybean meal, soybean oil, beer yeast, minerals, calcium dihydrogen phosphate, choline chloride, multivitamins for trionyx sinensis, coated vitamin C, sodium butyrate, curcumin, antimicrobial peptide and the like. The powdery formula feed for trionyx sinensis juveniles special for greenhouse breeding mode mainly provides various nutrients necessary for the growth on account of the growth characteristics of trionyx sinensis juveniles, has advantages of strong pertinence, comprehensive nutrition, excellent breading effect and lower feed coefficient, and effectively promotes the healthy and rapid growth of trionyx sinensis juveniles.

The invention provides a method for improving yellow sand trionyx sinensis egg production. The method for improving the yellow sand trionyx sinensis egg production is characterized in that parent trionyx sinensises at the age of 2-3 are selected according to a ratio of 4-6:1 of mother trionyx sinensises to father trionyx sinensises, and are placed into a pond special for being used for egg laying. 20-30g calcined lime is placed in each cube water in the pond, and the pond is designed to be filled with flowing water. From May to the end of August, a feeding method that a breeder feeds flour weevils and ampullaria gigas to soft-shelled trionyx sinensises in a first day, the breeder feeds fly maggots and fish meals to the soft-shelled trionyx sinensises in a second day, the breeder feeds the flour weevils and the fish meals to the soft-shelled trionyx sinensises in a third day, and the breeder feeds the ampullaria gigas and the fly maggots to the soft-shelled trionyx sinensises is regarded as a cycle to feed the soft-shelled trionyx sinensises repeatedly. A place for the soft-shelled trionyx sinensises to bask is arranged in the pond. The soft-shelled trionyx sinensises are enabled to bask for 30-60minutes per day, filament lamps are used as light sources in rainy days, and the filament lamps are turned on for 1-5h every night. The purpose of turning on the lamps is to attract insects at night, and if the soft-shelled trionyx sinensises see the insects, the soft-shelled trionyx sinensises can get out of water to prey on the insects naturally. Under the lamplight, the soft-shelled trionyx sinensises can absorb luminous energy to promote absorption of calcium. The egg production of the yellow sand trionyx sinensis is improved by 20-30% through the breeding method.

The invention discloses a feed additive for trionyx sinensis and a preparation method of the feed additive. For specific conditions of the trionyx sinensis, the feed additive is prepared through combination of modern bioprocesses according to a certain rule and on the basis of the theory of traditional Chinese herbal medicines, is nature in sources, can effectively increase the nutritional value of a basic feed, improve immunity of the trionyx sinensis, guarantee health of the trionyx sinensis, remarkably improve the flavor and nutritional value of the trionyx sinensis, take effects quickly, and greatly improve the quality of the trionyx sinensis within two months. In addition, the living bacterium additive is long in guarantee period and can be stored for 18 months at normal temperature with, and the living bacterium survival rate can still reach 90% or above.

The invention provides a horizontal spiral ribbon mixer and a technology for processing of trionyx sinensis feed by the same. The horizontal spiral ribbon mixer comprises a base and a cylindrical mixing barrel fixed on the base. A main shaft runs through the mixing barrel along the axis, one end of the main shaft is dynamically connected to a motor fixed on the base through a speed reducer, the other end of the main shaft is rotatably connected to the base through a bearing, a plurality of support rods are welded and fixedly connected to the main shaft, the support rods are arranged on the horizontal main shaft along the axis direction at an equal interval, the upper and lower support rods are in staggered arrangement, and internal spiral ribbons and external spiral ribbons are welded and fixedly connected to the support rods. The technology for processing of trionyx sinensis feed by the horizontal spiral ribbon mixer specifically includes the steps of: artificial feeding, raw material cleaning, preliminary mixing, ultrafine grinding, secondary mixing, and finished product packaging. The horizontal spiral ribbon mixer provided by the invention improves the material mixing efficiency. And the technology improves the feed uniformity and reduces the feed doping rate.

The invention discloses a drug for treating trionyx sinensis saprolegniasis caused by fungi, wherein the drug comprises gentian violet, sodium chloride, honeysuckle, cnidium monnieri, wild chrysanthemum, acorus calamus, garlic, tangerine peel, and rhus chinensis. The drug of the present invention provides the inhibition effect on saprolegniaceae fungi and can be used for treating trionyx sinensis saprolegniasis. In addition, the invention further discloses a preparation method of the drug.

A method and a device for improving male hatching rate of oviparity mainly solve the drawback that the male hatching rate of the current artificial incubation of the oviparity is low. The method comprises the following steps: arranging the hatching eggs after disinfected in a sealed pressure container before the artificial incubation; dipping the hatching eggs in the mixed liquid formed by mixing made drugs analogous with distilled water or normal saline; sealing the container, pressurizing to 2-3 atmospheric pressure, and keeping for 0.5 to 1 hour; taking the hatching eggs outside, then drying and entering the artificial incubation. The invention has a simple technology, little investment, good efficiency, high male hatching rate which reaches 65% to 80%, and can be widely applied to the artificial breeding of the oviparity, such as poultry, rare birds, snakes, tortoise and trionyx sinensis.

The invention relates to the technical field of aquaculture devices, in particular to a trionyx sinensis juvenile feed table for weighing feeding amounts. The trionyx sinensis juvenile feed table comprises a pontoon. The pontoon is used for being arranged at an aquaculture water surface in a floating manner, and a weighing device is arranged above the pontoon; the weighing device comprises a tray,the tray is arranged right above the pontoon and is hung at a lifting hook of an electronic crane scale by lifting wires, and a feed disc is arranged in the tray; the electronic crane scale is arranged at an end of a fixing frame by a suspension wire, the suspension wire is wound in a rolling device arranged at the end of the fixing frame, and the rolling device is used for rolling up and payingoff the suspension wire. The trionyx sinensis juvenile feed table has the advantages that tedious operation steps in existing feeding amount measurement and calculation procedures can be effectively simplified by the trionyx sinensis juvenile feed table, and feeding amount measurement and calculation effects can be effectively quickly and conveniently realized in aquaculture systems.

The invention discloses a formula of a trionyx sinensis skin fester disease treating traditional Chinese medicine. The trionyx sinensis skin fester disease treating traditional Chinese medicine comprises licorice root, rhus chinensis mill, garlic, honeysuckle, wild chrysanthemum, rheum palmatum, ash bark, herba houttuyniae, hawthorn, coptis chinensis, scutellaria baicalensis, cortex phellodendri, isatis tinctoria, alisma plantago-aquatica linn, angelica, seasoned orange peel, radix asparagi, forsythia suspensa, fructus chebulae, artemisia carvifolia, smoked plum, pangolin, fortune paulownia flower, cayratia japonica (thunb.) gagnep, solanum lyratum thunb, viola philippica and snakegourd root. The trionyx sinensis skin fester disease treating traditional Chinese medicine of the present invention can be used for treating skin fester diseases, and has characteristics of obvious treatment effect and no side effect, wherein although the garlic and other heavy flavor materials are used, other drugs are subjected to reasonable compatibility, such that the purposes of flavor removing and treatment effect remaining are achieved.

The present invention discloses a special compound feed for young trionyx sinensis, which includes the following raw materials: puffed corns, soybean protein powder, saccharomyces cerevisiae, milk powder, blood plasma protein powder, silkworm chrysalis meal, earthworm meal, bone meal, calcium hydrogen phosphate, coconut oil, lysine, methionine, threonine, tryptophan, zinc oxide, copper sulfate, chromium picolinate, citric acid, formic acid, lactose, portulaca oleracea, medicated leaven and complex enzyme preparation. The special compound feed for young trionyx sinensis can improve the immunity of the young trionyx sinensis, reduce the morbidity of the young trionyx sinensis, increase the intake of the young trionyx sinensis, and promote the growth of the young trionyx sinensis.

The present invention discloses a special digestion promoting feed for young trionyx sinensis which includes the following raw materials: puffed corns, puffed soybean, wheat bran, blood plasma protein powder, earthworm meal, fish meal, bone meal, whey powder, calcium hydrogen phosphate, coconut oil, lysine, methionine, threonine, tryptophan, glutamic acid, methionine chelated zinc, copper carbonate, copper chloride, chromium picolinate, malic acid, formic acid, fumaric acid, sucrose, fructooligosaccharide, portulaca oleracea, malt, pepsin, phytase, trypsin, edible salt, choline chloride and complex vitamins. The special digestion promoting feed for young trionyx sinensis can improve the digestion and absorption capacities of the young trionyx sinensis, reduce the morbidity of the young trionyx sinensis, promote the growth of the young trionyx sinensis and increase the use rate of the feed.

The invention discloses a method for mixed ecological breeding of trionyx sinensis and crucian in a paddy field. The method includes the steps of fry selection, paddy field requiring, fry throwing, feed management, disease prevention and treatment and final-period management. Compared with other breeding methods, the crucian and the trionyx sinensis are mixed to be bred, so that symbiosis of the crucian, the trionyx sinensis and the paddy field is utilized more reasonably, namely the crucian and the trionyx sinensis feed on injurious insects in the field, meanwhile, excrement of the crucian and excrement of the trionyx sinensis can provide fertilizer for paddies, the usage amount of pesticide is reduced, the throwing amount of fertilizer is also reduced, and the quality of the trionyx sinensis and the quality of the crucian are improved. Incomes of farmers are increased, the surrounding environment is further protected, and ecological harmony sustainable development is realized.

The present invention discloses an instant five-spice trionyx sinensis and a preparation method thereof. The raw materials comprise trionyx sinensis, c. m. var. sarcodactylis flesh, loquat flesh, momordica. charantia var. abbreviata ser flesh, an onion juice, an apple juice, fresh lotus leaf, saururus chinensis, hibiscus mutabilis, thesium chinense turcz, rheum palmatum, hot pepper powder, oyster sauce, five-spice powder, white spirit, gravy salt, peanut oil and the like. The instant five-spice trionyx sinensis has beneficial effects of delicious taste, rich nutrition, easy eat and food therapy health effect, wherein a variety of Chinese herbs are matched so as to provide effects of yin nourishing, kidney invigorating, metabolism promoting, and life prolonging.

The invention discloses an application of a feed additive to a soft-shelled turtle feed capable of improving meat quality and growing performance of soft-shelled turtles, and belongs to the technicalfield of feed additives. The feed additive comprises 10-45% of glycerol monolaurate, 10-30% of glyceryl dilaurate, 30-55% of glycerol monostearate and 5-15% of a carrier. The glycerol monolaurate, theglyceryl dilaurate, the glycerol monostearate are compounded according to the appropriate content and proportion, so that the feed additive is prepared. The feed additive can be used for trionyx sinensis during growth phase, the growing properties of the trionyx sinensis can be improved, and further, the breeding economic benefits are increased. The feed additive can notably increase the muscle of the trionyx sinensis and the content of crude proteins, the total content of amino acids, the content of necessary amino acids and the content of palatable taste amino acids in turtle edges of the trionyx sinensis can be notably increased, the nutritional quality, the mouth feel and the flavor of the trionyx sinensis are effectively improved, and contradiction between growth rate and quality issolved.

The invention relates to an anti-trionyx sinensis immune globulin monoclonal antibody, preparing the materials according to the following steps: (1) trionyx sinensis specificity antigen: collecting trionyx sinensis disposed in a bottle, condensing at a room temperature, standing the trionyx sinensis for 1.5-3h at 35-38 DEG C, standing for 8-12h at 3-6 DEG C again, centrifugating, taking supernatant, preparing a chromatographic column, crudely extruding ammonium sulfate water solution and diluting after dialyzing and concentrating, loading after filtering and eluting, wherein the firstly eluted protein IgM is the trionyx sinensis specificity antigen; (2) polyclonal antibody: sampling blood of the antibody immune animal, separating antiserum and purifying to obtain the polyclonal antibody; (3) hybridoma cell line: fusing myeloma cell with the splenocyte for generating the anti-trionyx sinensis immune globulin monoclonal antibody to obtain the hybridoma cell line; and (4) monoclonal antibody of anti-trionyx sinensis immune globulin: generating the monoclonal antibody by the hybridoma cell line. The trionyx sinensis immune serum titer is detected by the monoclonal antibody. The invention is suitable for researching turtle etiology and immunology.

The invention relates to the field of aquaculture, in particular to a takifugu obscures, coilia nasus schlegel and trionyx sinensis three-dimensional ecological breeding method, which comprises sevensteps of pond preparation, stocking of coilia nasus schlegel fingerlings and takifugu obscures, stocking of pseudorasbora parva and silver carps, bait feeding, daily management, and net pulling for catching. The invention fully utilizes the water space of a takifugu obscurus parent breeding pond, increases yield of the coilia nasus schlegel and trionyx sinensis under the conditions of not increasing the input, not reducing the takifugu obscures parent breeding density, and not affecting the takifugu obscurus parent gonad development, improves the water utilization rate of the pond, reduces theproduction cost, improves the economic benefit, reduces the feed cost, improves the quality of the trionyx sinensis, and makes the water quality of the pond more stable; the method of the invention is easy to operate and can be easily accepted and promoted.