Cucumber SNP marker and detection methods thereof
A detection method and technology for cucumber, which are applied in the fields of biochemical equipment and methods, recombinant DNA technology, and microbial determination/inspection, can solve the problems of difficulty, high cost, and restriction on the application of SNP markers in the identification of SNP marker sites. , to achieve the effect of shortening development and verification time and reducing inspection costs
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0028] A method for rapid identification of SNP loci
[0029] Step 1: Sequencing the target DNA interval on the cucumber parent materials to determine the candidate SNP sites; then the F 1 Sequencing of the target DNA interval in a single generation
[0030] In the present invention, the PCR marker is used to scan and screen the cucumber genome BAC library, and then obtain the related genome sequence linked with the M gene. All PCR reaction systems are: BAC library plasmid 20ng, bidirectional primers 0.2μmol / L, 200μmol / L dNTPs, 2mmol / L MgCl 2 , 1×Taq buffer, 0.5UT Taq DNA polymerase, the total reaction system is 10 μL, and Taq DNA polymerase was purchased from Promega Company. The amplification program was: 94°C for 3min; 40cycles, 94°C for 20s, 65°C for 30s, 72°C for 30s; 72°C for 5min, and the results were displayed by 1.5% agarose gel electrophoresis.
[0031] The present invention first uses the marker S_ME8SA7 to screen the expanded population, obtains two exchanged in...
Embodiment 2
[0037] Construction of genetically isolated populations and identification of exchanged individual plants
[0038] Step one, F 2 Segregation Population Construction
[0039] European greenhouse type inbred line H34 (male parent), hermaphrodite flower variety, the whole plant bears hermaphrodite flowers under normal growth conditions, the plant grows faster, mature fruit is spherical, dark green; South China type inbred line S52 (female parent) This), a unisexual flowering variety, in the early stage of plant growth (lower node position) male flowers are born, then male flowers and female flowers are distributed alternately, in the late growth period (higher node position) female flowers are completely born, the plant growth rate is slow, and the mature fruit is long. shape, white. F 1 Progeny, unisexual flowering plant (strong female plant), long strip-shaped fruit, dark green. Inbred lines S52, H34, F 1 Judgment criteria for offspring and unisexual flowering plants / bisex...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com