Multiplex amplification kit for cattle paternity identification and individual identification and application thereof
A paternity identification and compound amplification technology, which is applied in the field of compound amplification kits for cattle paternity identification and individual identification, can solve problems such as inability to identify cattle gender, and achieve improved overall identification ability, high resolution, and typing. accurate effect
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Embodiment 1
[0089] A detection method of a cattle paternity test and a multiple amplification kit for individual identification, comprising the following steps:
[0090] 1. Screening of genetic loci
[0091] According to the requirements of high polymorphism, non-linkage between markers, and easy genotyping, as well as the actual primer test results and locus arrangement requirements, 20 chromosomal STR loci and 1 Amel sex locus were screened out: UMN0108, BM2113, FJ232028 , SPS115, CSRM60, BM067, TGLA122, INRA023, BM1818, ILSTS006, UMN0929, Amelogenin, ETH225, BM1824, TGLA126, G18833, TGLA227, FJ232023, FJ232022, FJ232026, SPS113.
[0092] 2. Arrangement of gene loci
[0093] According to the above 21 loci, a unique locus arrangement and chemical fluorescent dye labeling method are designed: UMN0108, BM2113, FJ232028, SPS115, CSRM60 are the first group, and the fluorescent dye marker is 6-FAM; BM067, TGLA122 , INRA023, BM1818, ILSTS006, UMN0929 are the second group, the fluorescent dye...
Embodiment 2
[0111] The application of a compound amplification kit for cattle paternity testing and individual identification comprises the following steps:
[0112] (1) Take 5 blood spot samples from different cattle: The samples are provided by a public security bureau.
[0113] (2) Utilize the Chelex method to carry out the cattle genomic DNA extraction to the sample, use the kit of the present invention to carry out typing detection to the extracted sample, and compare all the site typing of 5 samples, as shown in Table 4:
[0114] Table 4
[0115] site name sample 1 sample 2 sample 3 Sample 4 Sample 5 UMN0108 72,74 74,74 74,74 84,84 74,74 BM2113 127,133 131,135 125,127 127,133 131,133 FJ232028 150,162 154,162 150,154 150,162 150,162 SPS115 215,219 207,209 207,209 213,215 215,219 CSRM60 250,266 254,264 264,264 256,264 246,250 BM067 117,119 117,119 117,133 117,117 117,123 TGLA122 153,155 155,167 155...
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