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Method for breeding double-limitation multi-silk-quantity domestic silkworm breed

A dual-limited, variety-based technology, applied in fishing, application, animal husbandry, etc., to achieve the effects of large workload, long breeding cycle, and poor selection reliability

Inactive Publication Date: 2013-05-08
JIANGSU UNIV OF SCI & TECH
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Problems solved by technology

[0006] Technical problem to be solved: The purpose of the present invention is to solve the technical problem of using molecular markers in the breeding of double-limited and multi-filament silkworm varieties in silkworms, and to provide a molecular marker-assisted selection method for breeding double-limited and multi-filament silkworm varieties breeding method

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  • Method for breeding double-limitation multi-silk-quantity domestic silkworm breed

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Embodiment 1

[0023] 1. Description of the materials used

[0024] Existing research results have shown that silkworm cocoon silk traits are controlled by multiple genes, and molecular markers at multiple sites have been screened (Wang Xiuye. QTL for silkworm cocoon silk-related traits and mapping of the scaleless gene nlw. Graduate student of the Chinese Academy of Agricultural Sciences Master's thesis, May 2010; Shuai Zhan, et al. An integrated genetic linkage map for silkworms with three parental combinations and its application to the mapping of single genes and QTL. BMCGenomics2009, 10:389).

[0025] (1) Restricted variety resources 755 (publicly known and public, "Chinese Silkworm Varieties" Agricultural Press, May 1987, p. 262);

[0026] 1. Source: The silkworm species bred by the Sericulture Research Institute of the Chinese Academy of Agricultural Sciences are no longer used in production and are only used as breeding materials.

[0027] 2. Main features: Chinese system, duality, ...

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Abstract

A method for breeding a double-limitation multi-silk-quantity domestic silkworm breed includes the following steps: using a multi-silk-quantity breed for spring 'Jingsong' as a recurrent parent, conducting hybridization to a stripe sex-limited character donor parent through a sex-limited breed '755' female individual which is saved as a source, and then obtaining a sex-limited breed JsQXian; using a multi-silk-quantity breed for spring 'Haoyue' as a recurrent parent, conducting hybridization to the stripe sex-limited character donor parent through the sex-limited breed '755' female individual which is saved as the source to conduct economical character selection to each generation in the breeding process, and then obtaining a sex-limited breed HyQXian; enabling the target material JsQXian and the target material HyQXian to conduct hybridization, and then directly obtaining the double-limitation multi-silk-quantity domestic silkworm breed. The method is adopted to select a genetic background of the recurrent parent and a target character of the donor parent in a marker-assisted selection mode, and the new double-limitation multi-silk-quantity domestic silkworm breed which is high in yield, good in quality, and labor-saving in the reproductive process can be bred within 3-4 years quickly and high-efficiency.

Description

technical field [0001] The invention relates to a method for breeding silkworm varieties, more specifically, to a method for breeding double-limited and multi-filament silkworm varieties by using a molecular marker-assisted selection method. technical background [0002] Sericulture and silk reeling have a history of more than 5,000 years in my country. It is one of the most distinctive traditional advantageous industries in my country and one of the few products that can dominate the international market. At present, my country's silkworm cocoon and raw silk production ranks first in the world First, they respectively account for about 70% of the world's total output. [0003] The silk industry not only plays an important role in increasing farmers’ income and expanding labor employment, but also the food of silkworms—mulberry trees also play an important role and significance in environmental protection and returning farmland to forests. Therefore, in many areas, ecological...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K69/04
Inventor 李木旺侯成香谭安江黄勇平
Owner JIANGSU UNIV OF SCI & TECH
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