111 results about "Crossover test" patented technology

The invention relates to three-class cross breed paddy multifunctional restorer molecule assistant breeding selection method that includes the following steps: selecting wide compatible gene material and taking polymer cross breed, taking molecule mark assistant selection to excellent individual, taking anther culture of the excellent individual containing at least four different targets gene to gain excellent material, and gaining multifunctional restore series after taking test cross, repeating test cross. The invention supplies an actual method for selective cultivating new species.

The invention relates to a breeding method of typically sterile non glutinous rice widely compatible sterile line with high stigma exposure rate, belonging to the field of rice breeding. In the invention, indica type temperature sensitive sterile line Peiai 64S serves as a female parent, conventional non glutinous rice variety Xiushui 110 serves as a male parent, the two are hybridized, direction selection is carried out on later generations; single plant with good agronomic trait, high stigma exposure rate and early blossom time is selected, test cross is carried out on the single plant by typically sterile non glutinous rice sterile line Chunjiang 16A dwarfing-sterile, meanwhile, widely compatible testing seeds are used to carry out wide compatibility appraisal, thus breeding fine material Chunjiang 95 with high stigma exposure rate, early blossom time, good wide compatibility and capacity of staying sterile; Chunjiang 16A dwarfing-sterile / Chunjiang 95 F1 serves as the female parent, and Chunjiang 95 serves as the male parent to carry out back cross; fertility appraisal in a generation by generation manner and continuous back cross is carried out on the later generations after the back cross to transform the later generations after the back cross into typically sterile non glutinous rice widely compatible sterile line Chunjiang 95A with high stigma exposure rate and early blossom time, corresponding maintenance line thereof is Chunjiang 95B. The method of the invention has the advantages of improved fertility stability and hybrid non glutinous rice seed production output of non glutinous rice sterile line.

The invention relates to a chicken breeding method, in particular to a breeding method capable of realizing feather color-based accurate self identification of the sex of a one-day chicken, which comprises the following steps: 1) crossing a pure golden Colombian feather cock with a white plymouth rock of a standard variety to obtain an F1 pure white feather cock; 2) back crossing the F1 pure white feather cock with a hen having a yellow feather male parent, and selecting a white-feather and black-tail cock and a white-feature and black-tail hen; 3) allowing a silvery-feather and black-tail cock and a white-feather and black-hen to propagate by themselves, selecting a silvery-feather and black-tail hen, eliminating golden-feature individuals, selecting a male typical Colombian feather individual, performing test cross of the male typical Colombian feather individual and the silvery-feather and black-tail hen, and eliminating heterozygous male non-silvery typical Colombian feather individuals from the offspring; and 4) crossing the silvery-feather and black-tail hen with a red-feather paternal line or yellow-feather paternal line having a golden-feather gene, wherein among the bred one-day chickens, those with white feathers are cock and those with golden feather are hens, and the self identification rate is 100 percent.

The invention discloses a method for cultivating a dominant-green-shin white feather broiler strain based on molecular assistant selection and belongs to the technical field of molecular breeding. According to the method for cultivating the dominant-green-shin white feather broiler strain based on molecular assistant selection, the method of molecular assistant selection is adopted for appraisal of a genotype of a broiler dominant white feather locus, so that the genotype of the white feather locus is rapidly judged, the dominant-green-shin white feather broiler strain with the II genotype is produced, complexity of test cross selective breeding is avoided, a generation interval is shortened, the breeding progress is accelerated, and therefore the cultivation cost is reduced. Meat of the dominant-green-shin white feather broiler strain cultivated through the method is fine and tender, taste of the meat of the broiler strain is delicious, the production performance of the broiler strain is high, color feather residues do not exist on a carcass, and therefore the carcass is beautiful. In addition, the strain can serve as a male parent and be hybridized with a high-yield laying hen strain or a fast-growing large broiler strain in a matched mode so that high-yield or fast-growing large dominant-green-shin white feather broilers can be produced respectively.

The invention discloses a cultivation method of broad-spectrum durable blast resistance rice, comprising the following steps: 1 a rice-blast resistance germplasm gene bank construction; 2 a basic colony construction; 3 intercross-shuttle selection- intercross; 4 pedigree shuttle high-pressure selection; 5 test cross identification; 6. start-point temperature identification; 7 resistance spectrum measurement, the germplasm material with complete group resistance frequency of above 90% to the known rice blast pathogene is kept to be broad-spectrum durable blast resistance rice. The broad-spectrum durable blast resistance rice is generated using group improvement technique, thus the rice has broad-spectrum persistent rice-blast resistance and copes with new rice-blast physiological strain resistance-gene and solves biparental homologous resistance of hybrid rice and linkage problem of resistance gene and disadvantageous gene, thus the broad-spectrum durable blast resistance rice of good quality can be obtained and both production and income can be increased.

The present invention uses rice intermedium type sterile line as female parent, uses intermedius type restoring line as male parent for cross breeding rice hybrid species. Said invention also includes that uses hsien rice sterile line and intermedius sterile line as female parent and uses rice hsien-keng crossed duplex haploid (DH) population and hsien-keng crossed recombinant inbred line (RIL) population as male parent to make test cross to obtain four hybrid populations of hybrid F1 generation. By detecting character of F1 and using hisen-keng molecule specific probe to detect keng-type index of F1 correspondent to male parent DH and RIL the F1 character-male-type keng-type index correlation can be obtained.

Method for producing YY super-male and XY physiological female common carps, with which YY-chromosome super-male common carp can be cultivated and androgenetic YY super-male common carp is produced, where microsatellite markers are used for paternity testing and test crossing confirmation. The YY common carp is crossed with normal female common carp to produce the progenies of only male common carp, and without sex identification, the juvenile male common carp with known sex are subjected to artificial sex reversal to produce XY physiological female common carp.

The invention discloses a breeding method for a high-yield new variety line of recessive white-feather gooses and application thereof. The method comprises the following steps of: selecting the high-yield white-feather gooses without broodiness or with weak broodiness, and eliminating dominant white-feather individuals and keeping recessive white-feather individuals A by the test cross or the molecular marker method; self-reproducing the male individuals and female individuals of A to breed the recessive white-feather gooses B; and breeding B again, eliminating dominant white-feather homozygote and heterozygote, self-reproducing the recessive white-feather high-yield individuals with homozygous genotype to expand the group, building a family and breeding the family until the high-yield recessive white-feather goose new variety line C is bred, and performing dualistic or polyphyletic mating by taking the C as a female parent and a colour-feather local goose as a male parent to produce colour-feather gooses or simulated local gooses.

The invention discloses a method for breeding a recessive nuclear sterile temporary maintainer line by utilizing a Brassica napus microspore culture technology, which comprises the following steps of: crossing by taking a good variety / strain as a female parent and taking a recessive nuclear sterile temporary maintainer line as a male parent to obtain an F1 generation; then carrying out inducing culture on the isolated microspore of the plant of the F1 generation by a Brassica napus microspore culture technology, thereby obtaining a DH strain; carrying out self cross on the DH strain and carrying out test cross on the heat exchange and a sterile line, wherein the test cross variety is completely sterile, and the corresponding DH strain is the recessive nuclear sterile temporary maintainer line. The good temporary maintainer line with a good genetic background can be rapidly obtained by the method and is applied to Brassica napus recessive nuclear sterile cross breeding.

The invention discloses a method for selecting and breeding Brassica napus recessive genic-male-sterile (GMS) line through the microspore culture technology and the cloning technology. The method comprises the following steps: a fine variety / line is hybridized with a recessive GMS line or temporary maintaining line to obtain the F1 generation; then the Brassica napus microspore culture technology is adopted to perform inducing culture to the isolated microspores of the F1 generation plant and obtain a DH strain; and then the DH strain with the sterile character is test-crossed with the temporary maintaining line, 100% of the tester strain is sterile, the corresponding sterile DH strain is the recessive GMS line; and then the cloning technology is used to rapidly obtain a large number of plants of the sterile line which are used for the complete sterile line production. By using the method of the invention, the sterile line with stable and excellent genetic background can be rapidly obtained and used in the Brassica napus recessive GMS cross-breeding.

The present invention discloses a method for breeding Brassica napus varieties and materials with a double haploid induction line of rapeseed, including: 1) determining target traits of breeding restorer lines, maintainer lines and conventional varieties of Brassica napus; 2) crossing or convergently crossing two or more Brassica napus with the target traits; 3) pollinating the cross or back-cross progenies with the double haploid induction line of rapeseed; 4) identifying the stability of the induced progenies; 5) performing test-cross identification or yield and resistance identification on the stable progenies; and 6) forming stable restorer lines and maintainer lines for cross breeding combination or for forming conventional varieties. The present invention can quickly and efficiently obtain rapeseed materials or conventional varieties with application value in breeding on a large scale, and lays a solid foundation for genetic breeding of Brassica napus, innovation of breeding resources, breeding of conventional rapeseed varieties, and breeding of new varieties of hybrid rapeseed. The method of the present invention can greatly improve the breeding speed and efficiency of hybrid or conventional varieties of Brassica napus, and reduce the human and material resources.

The invention discloses a method for breeding green-shelled egg laying ducks. The method is characterized by comprising the following steps: (1) collecting hatching eggs of male and female ducks from five or more than five towns of a central laying area, wherein the shape and appearance of the hatching eggs meet the variety, and the green-shelled egg rate of the hatching eggs is more than 40 percent; (2) cleaning, sterilizing and hatching the hatching eggs, eliminating unqualified ducklings to enable the variety and the appearance to be relatively consistent, then feeding mixed duckling groups until the ducklings are 120 days old, and establishing a basic green-shelled egg laying duck group according to a ratio of the male ducks to the female ducks by taking a family as a unit, wherein the ratio of the male ducks to the female ducks is 2:10; (3) making a comprehensive selection index, recording the egg yield, the daily egg number, the egg weight, the number of green-shelled eggs and the number of white-shelled eggs of each of at least thirty families in a determination process, eliminating white-shelled egg laying ducks from each generation, breeding a generation every two years, and performing test cross to improve the purity of green-shell genes and the egg laying performance so as to avoid mating of full sibs and half sibs, and breeding three to five generations to obtain a green-shelled egg product group.

The invention relates to a method for breeding a novel strain of anurous laying hens. The method includes steps of (1), selecting mutant anurous cocks and hens from indigenous chicken or laying hen resources and enabling the cocks and hens to perform self-reproduction to expand propagation; (2), selecting anurous cocks and hens from descendents of the expanded propagation, introducing blood relationship of high-yield laying hens into the anurous cocks and hens, selecting anurous cocks and hens in hybrid descendents, enabling the anurous cocks and hens to perform cross-breeding, and selectively reserving anurous cocks and hens in cross-bred descendents; (3), performing test-cross on the reserved anurous cocks and hens, evaluating the anurous cocks and hens and screening individual cocks and hens which are anurous genetic homozygous and enabling the individual cocks and hens to perform self-reproduction to expand propagation; (4), selecting the anurous genetic homozygous cocks and hens and improving the bodily form and appearance uniformity and the laying performance of the anurous genetic homozygous cocks and hens; (5), repeating executing the steps (3) and (4) until the novel strain of laying hens with the anurous character capable of being stably inherited. Appearance features of the anurous cocks and hens in cross-bred descendents meet requirements of breeding objectives. The method has the advantages that the bred novel strain of anurous laying hens is fast in growing speed and high in dressing percentage and egg numbers, and requirements of modern laying hen and broiler production can be met.

The invention provides a method for selecting and breeding a cabbage type rape cytoplasm sterile line through a rape doubled haploid inducible system. The method comprises the following steps: 1), determining restoring and maintaining relationships of stable strains; 2), according to test-crossing progeny trait specialization, performing backcross or multi-generation backcross on a temporary maintainer line and a stable sterile line; 3), pollinating the backcross progeny by using the rape doubled haploid inducible system; 4), identifying fertility of induced progeny; 5), selecting a perfect sterile single strain, and continuously using the rape doubled haploid inducible system to pollinate the sterile single strain; 6), after twice inducible pollinated progeny forms the sterile line, identifying the genetic stability and the sterility of the strain; 7), using the rape doubled haploid inducible system to pollinate, maintain, and select and breed stable new sterile lines with the maintainer line characteristics. According to the method provided by the invention, the cabbage type rape cytoplasm sterile line can be quickly selected and bred, and four generations can be obtained within two years at most; the genetic-stability cabbage type rape cytoplasm sterile line is obtained, the selecting and breeding speed of the cabbage type rape cytoplasm sterile line is greatly improved, the selecting and breeding speed and efficiency of the cabbage type rape hybrid variety are improved, and the labor and material investments are reduced.

The invention discloses a cucurbita pepo breeding method based on cold plasma treatment, and particularly relates to a method for obtaining cucurbita pepo with the functions of improving the seed germinating ability, increasing the height of seedlings, increasing the main root length, lowering a first female flower insertion node, improving the combining ability, antedating initial picking and increasing the biological yield. Collected cucurbita pepo seeds are planted and screened and are subjected to self-cross selective breeding to obtain cucurbita pepo parent materials, and seeds of the cucurbita pepo parent materials are subjected to the cold plasma treatment; the seeds of the cucurbita pepo parent materials subjected to the cold plasma treatment are subjected to excellent strain generation-adding selection, and an excellent variation self-cross line which has a target trait and is high in combining ability is selected by virtue of manners such as self cross, back cross and test cross; the excellent variation self-cross line is subjected to self-cross purification and rejuvenation to obtain stable male and female parents, and the male and female parents are hybridized to obtain an excellent cucurbita pepo hybridization assembly; by the combination of an anti-identification test and a variety comparison test, a new excellent cucurbita pepo variety is obtained finally.

The present invention discloses one kind of fast and efficient corn shuttle pedigree breeding process. By using American corn germ plasm 3163 as basic material and through four years of shuttle alternate planting in Sichuan Province in spring and summer and in Hainan Province in autumn and winter, excellent corn selfing line is bred. At the same time of selecting superior plants for selfing in Hainan Province, pollen and test variety parent 48-2 are test crossed; and via ecological comparison to select the excellent combination ZH002, regional test and production test for five years, outstanding corn hybrid Zhenghong-2 is bred. The shuttle breeding between the northern and the southern has fast breeding process and high selfing breeding efficiency.

The present invention relates to a method for breeding silkworm variety whose imaginal ala has no paraphyllium. Said breeding method includes the following several steps: parental selection, hybridization, backcross, test cross, continuous subculture and selfing so as to obtain two silkworm varieties whose imaginal ala has no paraphyllium.

A honglian-type hybrid japonica rice breeding method pertains to an application technique in the technical field of biology and modern agriculture, and is applicable to three-line japonica rice heterosis utilization. The honglian-type hybrid japonica rice breeding method comprises the following steps: honglian-type sterile cytoplasm of indica type rice is introduced to the japonica rice variety for transformation breeding of the honglian-type japonica rice sterile line; the honglian-type japonica rice sterile line is used for the test-cross screening or hybridization breeding of the honglian-type japonica rice restoring line; the honglian-type hybrid japonica rice is bred by selected mating of the honglian-type japonica rice sterile line and the honglian-type japonica rice restoring line. By breeding the honglian-type japonica rice sterile line and the corresponding restoring line to breed the honglian-type hybrid japonica rice, the honglian-type hybrid japonica rice breeding method solves the problem that the hybrid japonica rice BT sterile line in the existing production has unstable sterility and haploid cytoplasm and realizes three-line matching of the honglian-type japonica rice.

The invention provides an edible sunflower breeding method of sterile, maintainer and restorer lines. The method has the advantages of light workload and fast stability of sterile, maintainer and restorer lines. In a first year, an edible sunflower farmer species is used as a female parent, a cenospecies is used as a male parent for hybridization, and at the same time self-breeding is carried out for the cenospecies; in a second year, an excellent sterile single plant is separated from self-bred progenies of the cenospecies as a female parent, and an excellent single plant which is selected from hybrid progenies of the farmer specie and cenospecies is used as a male parent for test cross; self-breeding is carried out for a branch type fertile single plant which is selected from cenospecies segregating progenies; in a third year, materials of test cross varieties which show semi-sterility are eliminated; at the same time, self-breeding is continuously carried out for an excellent single plant which is selected from the branch type fertile progenies; in a fourth year, backcross in pairs is carried out for an excellent single plant which is selected from the sterile line and the maintainer line whose fertilities are stable; the branch type materials inbreed continuously; after 4-5 generations of backcross, the sterile line and the maintainer line whose agronomic properties are stable are generated; after 4-5 generations of self-breeding of the branch type fertile single plant, combining ability is detected, and a stable restorer line is obtained.

The invention discloses a cultivation method of an aromatic high-quality rice sterile line. A restorer line with Yunnan japonica rice gene and other different restorer lines are selected to conduct hybridization, a sterile plant is screened out from F2 generation, the sterile plant and the restorer line are test crossed, and the aromatic high-quality rice sterile line is obtained through backcrossing conservation breeding. According to the cultivation method of the aromatic high-quality rice sterile line, the rice variety with the Yunnan japonica rice gene or the restorer line is taken as a parent to conduct cross breeding with a hybrid rice restorer line with good characters, and the sterile plant in progeny is screened out from the F2 generation; by means of the method of the backcross, a rice sterile line which is high in sterile degree, good in restorability, good in character and stable in inheritance is bred. By means of the cultivation method of the aromatic high-quality rice sterile line, the restorer line with the Yunnan japonica rice gene is required by the sterile cytoplasm parent, a container line has big ears and big grains, sterile line sterile degree of transform breeding reaches to over 99.5%, percentage of chalky rice of the bred sterile line is within 10%, and the fragrance is achieved.

The invention discloses a method for breeding leaf mustard type rape varieties and materials by a rape double haploid inducing line. The method comprises the following steps: firstly, determining objective traits of the bred leaf mustard type rape varieties and materials; secondly, performing hybridization, convergent cross or backcross on at least two leaf mustard type rapes with the objective traits; thirdly, pollinating progenies obtained by hybridization, convergent cross or backcross by using the rape double haploid inducing line; fourthly, identifying the genetic stability of induced progenies, and realizing selfing breeding according to strains; fifthly, identifying the yield, resistance and quality traits of induced stable progeny strains; sixthly, determining the stable strains according to the yield, the quality traits and the resistance to form a conventional variety of leaf mustard type rape; seventhly, inducing stable progeny genetic strains to form a new leaf mustard type rape line and performing test cross on the new leaf mustard type rape line and a leaf mustard type rape sterile line to form a maintainer line or restorer line, or entering next round of breeding process of the varieties and materials. According to the method, the breeding speed and efficiency of a hybrid variety or a conventional variety of the leaf mustard type rape can be greatly improved, and labor force and material resources are reduced.

The invention discloses a hybrid rice breeding method using parents with close duration from seeding to heading and belongs to the breeding field of hybrid rice. The hybrid rice breeding method disclosed by the invention comprises: improving a rice sterile line and a restorer line by using different rice parents with close duration from seeding to heading and relatively remote genetic distance; carrying out sowing in different stages under different ecological environmental conditions to obtain a sterile line and a restorer line with close duration from seeding to heading and stable agronomic traits; then carrying out a test cross, planting a test cross F1 generation, and selecting a hybrid combination with excellent traits to obtain the hybrid rice with parents having close growing duration. The method ensures flower synchronization when the male parent and the female parent are sowed at a same stage, so that the reliability of production of hybrid seeds is improved; meanwhile, the mechanical operation in the field is further facilitated, the dependence on the labor force is reduced, the cost is lowered, and the efficiency of production of hybrid seeds is improved.

The invention discloses a seed selection method of sorghum A3 cytoplasmic male sterile line, The seed selection method comprises following steps, a test cross combination is obtained by taking A3TX398A as a cytoplasm source, and A1 and A2 sorghum restoring lines as male parents; F1 generation is cultivated, and fertility observation and identification is performed in blooming period so as to select a material possessing restoring?capacity on A1 and A2, and hold capacity on A3 cytoplasm; and the obtained material and A3 cytoplasm sourced TX398A are subjected to continuous backcross transformation for eight generations so as to obtain the sorghum A3 cytoplasmic male sterile line. The seed selection method of the invention is capable of providing a novel approach for seed selection of sorghum male sterile lines. The successful application of A3 cytoplasm in forage sorghum breeding and producing is capable of enriching hereditary basis of sorghum hybrid species, and increasing the types of sorghum hybrid species and the novel types of forage grass, and possesses significant importance in avoiding and eliminating the prevalence of sorghum cytoplasm specific diseases.

The invention belongs to the technical field of seed breeding. Japonica rice three-line sterile line plant repeated propagation stock producing method comprises the steps of: (1) individual selection, twin test cross and plant spacing comparison of a maintainer line and a sterile line; (2) plant spacing shed covering or greenhouse propagation of the maintainer line and the sterile line; (3) purity appraisal of the sterile line large samples; (4) open air propagation of the plant of the maintainer line and the sterile line; (5) purity appraisal of the sterile line large samples; (6) storage of the seeds in a refrigeratory of the maintainer line and the sterile line; (7) stock production of the sterile line; (8) purity appraisal of the sterile line large samples. The invention has the advantages of labor saving and time saving, high origin point of the seed purity, increased purity appraisal samples, large propagation coefficient and high production efficiency without purifying year-to-year.

The invention relates to a recurrent selection method for a dominant germplasm population of hybrid wheat. The recurrent selection method comprises the following steps: respectively carrying out half-sibling ear line phenotype recurrent selection on a CMS sterile line population and an R restoring line population; carrying out clustering analysis of genetic difference and heterotic group on diallel cross F1 combinations and parents thereof, wherein the parents comprise maintainer lines and restoring lines of male sterile lines CMS of wheat cells; carrying out CMS line half-slib recurrent selection by taking CMS sterile lines as female parents and B lines as male parents, selecting excellent sterile lines subjected to recurrent selection, and carrying out double haploid induction to obtainmale sterile DH lines; carrying out R line half-slib recurrent selection by taking dominant genic sterile Tal ear lines as female parents and R lines as male parents, selecting excellent sterile plants subjected to recurrent selection, and carrying out the double haploid induction to obtain fertile DH lines with restoring genes; carrying out test cross on the sterile DH lines and the fertile DH lines, selecting heterosis crosses of the lines according to the test cross result, and cultivating wheat hybrid varieties.

The invention relates to a method for breeding a brassica napus and mustard cytoplasm male sterility restoring line and belongs to the field of breeding of crops, which comprises the following steps of: carrying out intervarietal hybridization by using a mustard napus cytoplasm male sterile line WJS1A or a brassica napus and mustard cytoplasm male sterile line WNJ01A as a female parent and using green Chinese cabbages or Beijing cabbages as a male parent; selecting sterile plants with the characteristics of brassica napus from F2 to carry out selfing, carrying out test-crossing on the sterile plants serving as the male parent and the sterile line WJS1A to obtain male parent selfing seeds and test-crossing seeds and sowing the seeds; identifying the fertility of a test-crossing 1-generation in the flower season to eliminate a sterile combination and the male parent; selecting brassica napus plants which accord with the breeding aim from a fertile male parent line of a strong advantage combination to carry out selfing and carrying out test-crossing on the selected brassica napus plants and a brassica napus and mustard cytoplasm male sterile line; and repeatedly carrying out test-crossing for 3 to 4 generations until the brassica napus and mustard cytoplasm male sterility restoring line is bred. The method has very important significance of breeding the cytoplasm male sterility restoring lines of rapes and other cruciferae crops and utilizing the advantages of hybrids of the cytoplasm male sterility restoring lines of the rapes and other cruciferae crops.

The invention discloses a cultivation method of chromosomal-translocation triploid seedless watermelons. The cultivation method comprises the following steps of: (a) breeding of a male parent, namely crossing a chromosomal-translocation diploid watermelon breeding material with a diploid material suitable for serving as a seedless watermelon male parent, thus obtaining the male parent in a breeding method for four-generation continuous backcross, one-generation self cross and one-generation test cross; (b) breeding of a female parent, wherein mutating a diploid watermelon breeding material suitable for being mutated into tetraploid watermelons into tetraploid watermelons by a colchicine solution with the mass concentration of 0.2%, thus obtaining the female parent by self-crossing fertile restoration for 3-4 generations; and (c) performing cross matching on the female parent and the male parent to obtain the chromosomal-translocation triploid seedless watermelon variety through variety tests. The cultivation method disclosed by the invention has the advantages that chromosomal translocation and 3X double abortion are superposed, so that the breeding difficulty of more colored and abortive grains of 3X seedless watermelons is effectively overcome; and when the chromosomal-translocation triploid seedless watermelons are planted in protected areas and areas with large temperature difference at day and night, the colored and abortive grains do not occur, so that the planting range is expanded.

The invention discloses a large scale producing method for all female mandarin fishes. The method includes the following steps: S1, the breeding processing for sex reversal of mandarin fishes: sex reversal of mandarin fishes is performed by adopting the method that bait fishes which are fed with forage mixed with 17alpha-methyl testosterone hormone are ingested by the mandarin fishes in ten days after the mandarin fish fries start feeding and before the differentiation of the sexual gland; S2, the sexual gland identification for the sex reversal individuals; S3, the test cross for the sex reversal individuals; S4, the production for all female mandarin fish fries. The sex reversal of mandarin fishes is performed by adopting the method that the bait fish which are fed with forage mixed with17alpha-methyl testosterone hormone are ingested by the mandarin fishes. The technology of inducing sex reversal of mandarin fishes with high efficiency is established to cooperate with the corresponding biological sex sexual gland microscopic identification technology and the genetic sex test cross identification technology to provide technical support for the control of the mandarin fish sex and the all-female breeding. The sex reversal of the masculinization of 99% of female fishes of mandarin fishes can be realized by the provided method, the female proportion of all-female mandarin fishproduction can reach 100%, and all-female mandarin fishes have obvious growth advantages compared with common breeding mandarin fishes so that the method has an excellent application prospect.

The invention discloses a quick cultivation method of a sterile line of three-line hybrid rice. The quick cultivation method comprises the steps of hybridizing F1, which is generated by hybridizing a new rice species P and an improved maintainer line B and serves as a male parent, and a test cross offspring of the new rice species P and a sterile line A when the fertility of the test cross offspring of the new rice species P and the sterile line A is identified and the fertility of the test cross offspring sterile line is kept, and selecting single plants tending to target characters from F2, which is generated by hybridizing the new rice species P and the improved maintainer line B, and later generations to be continuously hybridized in pairs with selected sterile plants generated by re-hybridization of the test cross offspring of the new rice species P and the sterile line A so as to continuously feed genetic components of the single plants of the maintainer line into the sterile line, so that the sterile line can be quickly stabilized, and the yielding ability of the sterile line is improved. According to the method, a parent is subjected to restoring and maintaining relationship detection in the initial stage and transformation of sterile cytoplasms is executed at the same time; during selection of segregative generations, the transformation of the sterile cytoplasms is also executed, so that the breeding period is greatly shortened.

The present invention is cross breeding process of cotton hybrid utilizing cytoplasmic male sterile. Pest and disease resisting gene is transferred into sterile line to breed sea island cotton type cytoplasmic male sterile line and upland cotton type cytoplasmic male sterile with resistance to cotton blight, bollworm and pink bollworm separately. Through further hybridization and backcross, the sea island cotton type restoring line and the upland cotton type restoring line with strong cytoplasmic male sterility restoring capacity are produced separately. Through test cross and screening with sea island cotton type sterile line and upland cotton type restoring line or upland cotton type sterile line and sea island cotton type restoring line, combined advantageous hybrid F1 with high yield, high quality and high resistance is bred. The present invention has relatively low cost of producing hybrid.