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Molecular marking supplementary breeding method for multi-purpose restoring series of three-series hybrid rice

A molecular marker-assisted, three-line hybrid rice technology, applied in biochemical equipment and methods, microbial determination/inspection, etc.

Inactive Publication Date: 2006-09-13
RICE RES ISTITUTE ANHUI ACAD OF AGRI SCI
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  • Abstract
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  • Application Information

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Problems solved by technology

[0007] With the development of modern rice molecular biology, molecular markers closely linked to rice cytoplasmic male sterility restorer genes and wide-compatibility genes have been continuously screened out. However, due to experimental conditions and information communication, the actual It is difficult for field breeding scientists to directly use these molecular markers, resulting in the failure of these molecular markers to be widely used in the selection of broad-spectrum wide-compatibility restorer lines

Method used

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  • Molecular marking supplementary breeding method for multi-purpose restoring series of three-series hybrid rice
  • Molecular marking supplementary breeding method for multi-purpose restoring series of three-series hybrid rice
  • Molecular marking supplementary breeding method for multi-purpose restoring series of three-series hybrid rice

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Embodiment Construction

[0018] Now take the breeding of the multi-purpose restorer line mainly of the wild type as an example, which is described as follows.

[0019] 1. Prepare polymerization and hybridization

[0020] Shuhui 527 / / / 02428 / Yangdao No. 6 / / Peiai 64 / C418. Among the parents used in the aggregation exchange, Shuhui 527 is a wild-battered indica restorer line, which has the characteristics of strong resilience (including Rf3 and Rf4 genes), high combining ability, high quality, good plant and leaf shape, and relatively resistant to rice blast; 02428 It is a japonica rice wide-compatibility material with good plant and leaf shape and S5-n wide-compatibility gene; Yangdao 6 is a restorer line of Honglian indica rice, which has strong resilience (including Rf5 and Rf6 genes), high combining ability and high quality Excellent plant and leaf shape, strong stress resistance and other characteristics; Peiai 64 is a wide-compatibility indica rice material with good plant and leaf shape and S5-n wi...

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Abstract

The invention relates to three-class cross breed paddy multifunctional restorer molecule assistant breeding selection method that includes the following steps: selecting wide compatible gene material and taking polymer cross breed, taking molecule mark assistant selection to excellent individual, taking anther culture of the excellent individual containing at least four different targets gene to gain excellent material, and gaining multifunctional restore series after taking test cross, repeating test cross. The invention supplies an actual method for selective cultivating new species.

Description

1. Technical field [0001] The present invention relates to a method for breeding multi-purpose restorer lines of three-line hybrid rice. Specifically, molecular marker technology is used to assist in the aggregation of restorer genes and wide-compatibility genes from different sources to breed multi-purpose restorer lines of three-line hybrid rice. . 2. Background technology [0002] Cytoplasmic male sterility is the basis for utilization of heterosis in crops. my country has achieved great success in hybrid rice breeding using cytoplasmic male sterility. According to the different sources of cytoplasm, the cytoplasmic male sterility of rice currently used in production can be divided into wild type, dwarf type, Oka type, D type, Indonesian paddy field type, K type, red lotus type, and bag type according to the source of cytoplasm. Taiwan type, Dian 1 type, Ma Xie type, etc. According to the relationship between cytoplasmic male sterility recovery and maintenance, there ar...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
Inventor 易成新杨剑波李莉倪大虎汪秀峰赵伟宋丰顺陆徐忠张毅宁志怨宣云
Owner RICE RES ISTITUTE ANHUI ACAD OF AGRI SCI
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