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Method for breeding recessive nuclear sterile temporary maintainer line by utilizing Brassica napus microspore culture technology

A technology for microspore culture and Brassica napus, which is applied in the field of breeding recessive genital sterile temporary protection lines, can solve the problems of long breeding cycle, heavy workload, and difficult breeding, and achieve shortening and shortening of the breeding cycle The effect of the breeding cycle

Inactive Publication Date: 2010-07-07
SHANGHAI ACAD OF AGRI SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] However, since the temporary insurance line is controlled by three pairs of recessive genes (aabbrfrf) and is fertile, there are no performance traits that can be used for selection. Therefore, extensive test crossing is required, because it is controlled by three pairs of recessive genes and separates the population. Large, multiple and complex genotypes, resulting in a large workload for testcross and testcross species identification, long breeding cycle, and difficult breeding

Method used

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  • Method for breeding recessive nuclear sterile temporary maintainer line by utilizing Brassica napus microspore culture technology
  • Method for breeding recessive nuclear sterile temporary maintainer line by utilizing Brassica napus microspore culture technology
  • Method for breeding recessive nuclear sterile temporary maintainer line by utilizing Brassica napus microspore culture technology

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Embodiment 1

[0033] f 1 Configuration

[0034]The temporary insurance line M-6029, whose genotype is aabbrfrf, is the temporary insurance line of recessive genetic male sterile 20118A, which was passed consecutively in the test cross combination of recessive genetic male sterile 20118A by researcher Sun Chaocai of Shanghai Academy of Agricultural Sciences in 1999 Self-bred, test cross breeding, erucic acid content of 0.89%, glucosinolate content of 25.36μmol / g, excellent agronomic traits, high general combining ability and special combining ability.

[0035] Huyou 15, selected and bred by researcher Sun Chaocai of the Shanghai Academy of Agricultural Sciences, is the first Brassica napus variety in Shanghai that has passed the national variety approval and obtained the national plant variety protection, and has been listed by the Ministry of Science and Technology and the Ministry of Agriculture One of the national-level key promotion varieties, the oil content is 42.43%, the erucic acid ...

Embodiment 2

[0038] Breeding of temporary insurance line M-6477

[0039] 1. Donor F 1 Microspore culture of material

[0040] During the flowering stage of rapeseed in March 2005, the donor material F 1 (Huyou 15×Linbao line M-6029) was cultured with microspores, and a total of 379 embryoid bodies were obtained, and 282 individual plants were obtained through induction culture, of which the doubled plants were 76 DH lines, which were identified by testcross , found that there are 8 DH strains with genotype aabbrfrf.

[0041] 2. Identification of M-6477 of Temporary Insurance Department

[0042] The above 8 DH strains with the genotype of aabbrfrf were tested for their agronomic and quality traits, and a DH strain with good agronomic traits, good quality traits, and oil content ≥ 45% was obtained, which was named as Linbao strain M-6477.

[0043] 3. The regional test performance of the hybrids prepared by using the temporary insurance line M-6477

[0044] In 2008, the Shanghai Academy...

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Abstract

The invention discloses a method for breeding a recessive nuclear sterile temporary maintainer line by utilizing a Brassica napus microspore culture technology, which comprises the following steps of: crossing by taking a good variety / strain as a female parent and taking a recessive nuclear sterile temporary maintainer line as a male parent to obtain an F1 generation; then carrying out inducing culture on the isolated microspore of the plant of the F1 generation by a Brassica napus microspore culture technology, thereby obtaining a DH strain; carrying out self cross on the DH strain and carrying out test cross on the heat exchange and a sterile line, wherein the test cross variety is completely sterile, and the corresponding DH strain is the recessive nuclear sterile temporary maintainer line. The good temporary maintainer line with a good genetic background can be rapidly obtained by the method and is applied to Brassica napus recessive nuclear sterile cross breeding.

Description

technical field [0001] The invention relates to the field of Brassica napus breeding, in particular to a method for breeding recessive genital male sterile temporary insurance lines by utilizing the microspore culture technology of Brassica napus. Background technique [0002] The isolation technology of free microspores of Brassica napus is simple, and the efficiency of obtaining embryoid bodies and regenerated seedlings by free microspore culture technology is significantly higher than that of anther culture, and microspore culture is a good method for studying the differentiation conditions of pollen cells, embryogenesis and morphogenesis mechanism. A relatively ideal method, so this technology has been generally favored by rapeseed breeders in various countries. Over the years, many scholars have conducted extensive research on the composition of the medium for microspore culture, the size of buds, the temperature change during the microspore culture process, and the inc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/04A01H4/00A01H1/02
Inventor 杨立勇孙超才王伟荣周熙荣张智奇周音李延莉蒋美艳庄静顾龙弟
Owner SHANGHAI ACAD OF AGRI SCI
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