198results about How to "Efficient cultivation" patented technology

A method of producing a prenyl alcohol, comprising creating a recombinant by transferring into a host a recombinant DNA for expression or a DNA for genomic integration each comprising a prenyl diphosphate synthase gene or a mutant thereof, culturing the resultant recombinant, and recovering the prenyl alcohol from the resultant culture.

An object of the present invention is to provide a spongelike structure or a powder having fibers three-dimensionally arranged therein with high dispersibility, whose apparent density can be designed depending on the purpose or utility, as well as a process producing them. A fiber dispersion in which fibers having a number mean diameter in a predetermined range are dispersed in a dispersion medium, and this fiber dispersion is dried to remove the dispersion medium, thereby, a spongelike structure and a powder are produced.

The invention aims at providing a whole-genome-selection-based method for breeding improved disease-resistant fish varieties, thereby overcoming defects in the traditional breeding technology, providing a molecular breeding method for cultivation of improved disease-resistant high-yield fish varieties, solving a problem of lack of a whole-genome selection method in the existing fish breeding industry, providing a technical means for improved disease-resistant fish variety breeding of the fish breeding industry, realizing upgrading of the fish breeding technique, and promoting rapid development of the fish breeding industry. The disease-resistant fish offspring obtained by using the method provided by the invention has the enhanced disease-resistant capability. An experiment result demonstrates that the survival rate from infection and breeding survival rate of a disease-resistant fish offspring that is bred based on the whole genome selection method are higher than those of the control group by 20% to 30%. Using the method provided by the invention, the improved disease-resistant fish breed can be bred rapidly and efficiently; and the disease-resistant capability and breeding survival rate of fish can be enhanced. Therefore, the method provided by the invention has the great application value and broad promotion prospects in the fish breeding industry.

An apparatus which includes a growing container in which plants are hydroponically grown on a support medium is disclosed. The growing container includes a plurality of openings in which seals are installed. The seals include holes through which the plants grow. When seeds are planted, a hollow tube is placed through the seal hole to maintain the seed in position. After the seed germinates, it grows through the tube. As continued growth expands the plant's stem to the size of the tube, the tube breaks apart and does not constrain future plant growth. Moreover, as the plant grows further, the seal hole expands to accommodate the plant stem. The seal surrounds and contacts the plant stem to reduce evaporation of nutrient solution from the growing container and to keep insects and contamination out of the growing container. A holding container is positioned above the growing container and coupled to the growing container through a manifold arrangement. The holding container may be quickly filled with a single, measured nutrient solution charge for the growing container. Then, a valve is opened, and the manifold arrangement slowly distributes the nutrient solution to the growing container where it is substantially absorbed by the support medium and wicked to the vicinity of the plant within the growing container.

The present invention provides a new nucleic acid molecule which encodes a polypeptide that is able to convey a resistance to a pathogen, in particular to “Beet Necrotic Yellow Vein Virus” in a plant, in particular a plant of the Beta genus, in which the polypeptide is expressed, and also a preferred nucleic acid molecule encoding the RZ-3 gene of Beta maritima, derivatives and homologues thereof. Further aspects of the invention include vectors, transgenic plant cells, transgenic plants, methods for production thereof, and methods for identifying a resistance-conveying nucleic acid molecule.

The invention discloses a high-yield high-efficiency technology for cultivating sinonovacula constricta in a pond, which is characterized by comprising the steps of (1) pond selection, (2) pond modification, (3) pond treatment, (4) treatment of an algae cultivating pond and (5) selection of sinonovacula constricta larva. By means of modification of the cultivating pond and changing of an existing blind and unsuitable fertilizing-feeding mode, growth speed of sinonovacula constricta cultivating in the pond is greatly improved, a cultivating period is shortened, two-batch yield of the pond sinonovacula constricta in one year is achieved, cultivation survival rate, specification and yield are improved remarkably, and high-quality, high-yield and high-efficiency pond cultivation of the sinonovacula constricta is facilitated.

The invention discloses a high-yield planting method for preventing and treating corn stem rot in semi-arid regions, belonging to the technical field of agricultural planting and culturing. The high-yield planting method comprises the following steps: land selection and preparation, seed selection, seed treatment, ridging, base fertilizer application, seeding, drop irrigation, water feeding, weeding, membrane lamination, top application, field management, corn stem rot prevention, harvesting and the like. According to the high-yield planting method, the problem that the seeding emergence rate of corns in the second year is influenced by the total straw application of straws is solved; by processing corn seeds, the emergence rate can be improved by more than or equal to 5%, and the straw turnover in the second year is not influenced; the preventing and treating effect of the corn stem rot reaches more than or equal to 70%; water is saved by more than or equal to 40%, and the corn yield is increased to be 800-1000 kilograms per hectare; adverse factors caused by the ecological environment to the agricultural production are overcome, the traditional agricultural production mode is changed, the unit yield is increased, the yield and the efficiency are increased, and the effects of reducing wind erosion of soil, protecting the environment and improving the drought resisting capacity and the production stability of crops are achieved.

The invention provides a method for planting Chinese yam. The method comprises the following steps of (1) land selection, seed selection and disinfection, (2) soil preparation and furrowing, (3) sowing, (4) bud picking, base fertilizer applying and top application, (5) water management, (6) pest control and (7) harvesting. The method for planting the Chinese yam solves the practical problems, hindering large area development of the Chinese yam, that diseases are easy to infect during close planting, top application difficulty is large, furcation occurs in tuberization, has the advantages of being wide in applicability, straight and even in tuberization of planted Chinese yam, high in per unit yield and good in quality, and can achieve high-yield, high-quality and high-efficiency cultivation.

The invention discloses Sebacinales sp. LQ capable of promoting germination of seeds of medicinal Dendrobium officinale andDendrobium flexicauleto form seedlings; and the Sebacinales sp. LQ is preserved in China Center for Type Culture Collection under the preservation number of CCTCC NO: M 2019744. The invention further provides morphological, physiological and biochemical characteristics of theSebacinales sp. LQ. According to the morphological, physiological and biochemical characteristics, as well as nucleotide sequence and phylogenetic analysis results, the Sebacinales sp. LQ. is a fungusstrain pertaining to the Sebacinales sp.. The Sebacinales sp. LQ. can be used for promoting germination of seeds of Dendrobium officinale and seeds of Dendrobium flexicaule to form seedlings; and theSebacinales sp. LQ. has relatively high specificity. Thus, the seeds of the Dendrobium officinale and the seeds of the Dendrobium flexicaule can be separatelyinoculated with the Sebacinales sp. LQ. so as to obtain high-quality symbiotic seedlings, thereby realizing efficient cultivation of seedlings by symbiotic germination; and moreover, symbiosis can be used for seedling raising in nursery or direct seed sowing on trunks in the field. Compared with tissue culture, symbiotic culture has the advantages of being low in cost, high in survival rate in the field, free of the problem of tissue degradation and so on; and thus, the symbiotic culture has application values in obtaining of symbiotic seedlings in replacement of tissue culture.

The invention discloses an efficient fertilizer contributing to reduction of accumulation of a chromium heavy metal pollutant in a vegetable. The fertilizer comprises the following components, by weight part: betaine, activated nanometer attapulgite, rice bran, powder or fermented substances of stem-type vegetables with strong fiber, cationic chitosan, germinated brown rice, microzyme, melatonin, an anion-modified macro-molecule chitosan heavy metal adsorbent, and a proper amount of mineralized micromolecule activated water. Melatonin and betaine treated with mineralized micromolecule activated water match activated nanometer attapulgite to prepare factors for self-cleaning of a chromium heavy metal. The factors easily enter the inside of cells, quickly participate into living movement of plants, promote plant bodies to absorb and utilize heavy-metal-chromium-type substance, and further promote living movement of the bodies, thereby reducing pollution. The fertilizer matching a nutrient solution treated through mineralized micromolecule activated water, and main materials can effectively convert heavy metals left in the vegetable, thereby having a function of self-cleaning of heavy metals.

The present invention can feed substrates without waste to generate seed sludge with high bacterial cell concentrations and can start up operation within a short time, in cultivating anaerobic ammonium oxidizing bacteria with ammonium and nitrite as substrates. Equipment for cultivating anaerobic ammonium-oxidizing bacteria, which cultivates, in a cultivation tank, novel anaerobic ammonium-oxidizing bacteria that anaerobically denitrify nitrite and ammonium used as substrates, comprises an ammonium feed device which feeds ammonium at a given concentration into the cultivation tank, a nitrite feed device which feeds nitrite at a given concentration into the cultivation tank, and a control device which controls a feed rate Y of the substrate.

The invention relates to a method for raising seedlings of rice. The method comprises the following steps: step 1: seed soaking and germination; step 2: sowing; step 3: darkening processing; step 4: management of temperature and humidity in a greenhouse; step 5: management before transplanting. The method for raising seedlings of rice has the following beneficial effects: matrixes made by cassava leftovers during alcohol production are fully used so that production cost is greatly reduced; the matrixes are good in water storage performance and water retention performance so that moisture management during the process of raising seedlings is simplified; the method is easy to operate, manage and labor and time-saving; seeding trays are good in consistency with white root hair and robust seedlings; during transplanting, missing and breaking of seedlings are avoided; and the survival rate of seedlings is high.

The present invention provides a new nucleic acid molecule which encodes a polypeptide that is able to convey a resistance to a pathogen, in particular to “Beet Necrotic Yellow Vein Virus” in a plant, in particular a plant of the Beta genus, in which the polypeptide is expressed, and also a preferred nucleic acid molecule encoding the RZ-3 gene of Beta maritima, derivatives and homologues thereof. Further aspects of the invention include vectors, transgenic plant cells, transgenic plants, methods for production thereof, and methods for identifying a resistance-conveying nucleic acid molecule.

The invention relates to an efficient coprinus comatus cultivation method. The method comprises the steps that production strains are prepared according to a conventional method, raw materials are composed of waste mushroom residues, composts are prepared through fermentation, the composts are directly mixed with the production strains, then the mixtures are bagged and cultivated to obtain bacteria sticks, a greenhouse is completely disinfected, cultivation beds are built while the bacteria sticks are cultivated by earthing up, a partition ditch is built between every two adjacent beds while the cultivation beds are built, the partition ditches are filled with water reasonably step by step in the production and management process, and the yield is continuous and stable due to sufficient water. By means of the method, waste is utilized greatly and turns into wealth, the composts are directly mixed with a large number of production strains after being fermented, hyphae rapidly grow all over after the strains are germinated, and the production cycle of the bacteria sticks is shortened by 15-20 days. In the production and management process, the irrigation type fruiting management is implemented, fruit body surface water accumulation is effectively prevented, mushroom surface bacterial diseases are effectively controlled, and the efficient, time-saving and labor-saving cultivation effect is achieved.

In order to provide an efficient hydroponic culture system, the present invention provides a hydroponic culture system provided with: a plurality of water tanks which extend in a predetermined direction; a plant holding plate which holds plants and are disposed to extend over the plurality of water tanks; and at least one support mechanism which is provided between adjacent water tanks among the plurality of water tanks, wherein each of the plurality of water tanks is configured to contain a culture solution required to grow the plant, and the support mechanism supports the plant holding plate above the plurality of water tanks.

The present invention belongs to the field of slow-release fertilizers, and specifically discloses a melon high-efficiency slow-release fertilizer with degerming insecticidal effects, the melon high-efficiency slow-release fertilizer with degerming insecticidal effects is characterized by comprising the following components in parts by weight: garlic polysaccharide sulfide derivative, attapulgite, polyvinyl alcohol, a crosslinking agent, polyglutamate hydrogel, mesoporous silica nanoparticles, salicylic acid, toosendanin, pyrethrum, tea polyphenols, composite microbial agents, sodium selenite, compound sodium nitrophenolate, viscous konjac flour, residue of furfural, brown sugar residue, and amino acids and the like. The garlic polysaccharide sulfide derivative and the attapulgite are used as basic coating materials, the micron size composite hydrogel prepared from the polyglutamate hydrogel and different sizes of mesoporous silica nanoparticle matters are added into the coating materials, the pore size of the coating materials are enriched, the dynamic slow-release performance and nutrient use efficiency of the fertilizer are improved, the fertilizer efficiency regulation property is ensured, the melon high-efficiency slow-release fertilizer promotes the melon to absorb and utilize different nutrients, and promotes the efficient cultivation.

A bioreactor for cultivating tissue cells comprises a vessel containing a gas phase and a liquid phase therein, at least a substrate on which the tissue cells are attached, and a movable shaft to which the substrate is fixed. The movable shaft carries the substrate into and out of the gas and liquid phases so as to apply shear stress to the tissue cells.

The invention discloses a method for cultivating cotton in saline-alkali soil, and relates to the field of technologies for cultivating crops. The method is characterized by comprising steps of plowing the soil to press salt; pressing the salt by the aid of water; additionally applying organic fertilizers to the saline-alkali soil; sowing seeds in due time; covering the soil by mulching films; placing seedlings in the soil in due time; reducing branches of cotton and pruning the cotton; uncovering the mulching films timely, watering the cotton and applying additional fertilizers to the cotton; performing reasonable chemical control; timely performing insect disease prevention on the cotton. The method has the advantages of simplicity, clear steps and good cultivation effect.

The invention discloses an efficient fertilizer helpful for raising nitrogen utilization rate of spinach. The efficient fertilizer comprises the following ingredients: melatonin, a water-soluble silicon fertilizer, humic acid, mannoproteins, yeast, soybean curb residue, alga residue, high temperature calcined oyster shell porous powder, nanometer black bone charcoal, oxidized sodium alginate, a proper amount of mineralized small molecular activated water, etc. By using melatonin, the water-soluble silicon fertilizer, mineralized small molecular activated water and nanometer black bone charcoal, a nitrogen utilization promoter is prepared. Small molecular water-soluble silicon powder participates in cell metabolism to promote synthesis of an amino acid substance from a nitrogen substance. Melatonin accelerates photosynthesis and metabolism of plants and promotes utilization of nitrogen and oxalic acid. Thus, enrichment of nitrogen and oxalic acid by spinach is minimized and efficient cultivation of spinach is realized. Meanwhile, in cooperation with a nutrient solution processed with mineralized small molecular activated water and main materials, the fertilizer of the invention has rich nutrients and can effectively minimize enrichment of nitrogen and oxalic acid in spinach and raise quality of spinach.

The invention discloses a high-efficiency slow-release fertilizer for high-efficiency cultivation of coix seeds in saline land. The high-efficiency slow-release fertilizer is prepared from, by weight, oyster shells, polylactic acid, activated nano attapulgite, viscid loess, almond residues, tea waste residues, rice chaff ash, modified sea mud, perlite, vermiculite powder, VA mycorrhiza, saccharomycetes, exogenous proline, ardealite, a proper amount of hydrogen peroxide, a proper amount of ammonia water and the like. The almond residues, the tea waste residues, the rice chaff ash, the modified sea mud, the perlite, the vermiculite powder and the like are matched with nutrient substances to prepare the coated slow-release fertilizer after fermentation, and the fertilizer contains rich fermented micromolecular organic matter, the inorganic moisture regulator vermiculite powder and the like itself, is easily absorbed and utilized by plants. In addition, the pH value of saline and alkaline land is automatically regulated by adopting the inorganic moisture regulator, and the resistance of the coix seeds in the saline land is improved. A coating layer capable of supplying oxygen and dynamically regulating pores is adopted, the fertilizer efficiency time is long, organic matter nutrients can be released for a long time, high-efficiency cultivation of the coix seeds in the saline land is promoted, and the high-efficiency slow-release fertilizer can be widely applied and popularized.

An aerobic granular sludge integrated sewage treating device is disclosed. The device comprises a water feeding pipe, aeration systems and a sludge discharging perforated pipe. The device also comprises a cylindrical tank body. The center of the cylindrical tank body is provided with a cone reaction drum. The periphery of the cone reaction drum is provided with cylindrical separation plates. The cone reaction drum is the first reaction chamber. The space between the cone reaction drum and the cylindrical separation plates is the second reaction chamber. The space between the cylindrical separation plates and the cylindrical tank body is the third reaction chamber. Each reaction chamber is provided with a plurality of layers of mesh plates which are arranged horizontally. The bottom of the cone reaction drum is provided with a water-gas mixing short pipe. The water-gas mixing short pipe is connected to a gas feeding pipe. The lower end of the water-gas mixing short pipe is provided with a frustum cone-like water feeding cavity. The outlet of the water feeing pipe is horizontally arranged, is in the frustum cone-like water feeding cavity, and is provided with a nozzle. The lower end of the cylindrical tank body is provided with a cone. The bottom of the cone is connected to the sludge discharging perforated pipe. The top edge of the inner wall of the cylindrical tank body is provided with a water discharging groove. The device has characteristics of simple configuration, construction convenience, small occupied space, convenient running, good quality of discharged water, low manufacturing cost, and low running cost.

The invention discloses a plant yellow dwarf resistance-associated protein, a coding gene and application thereof. The protein provided by the invention is a protein (a) or (b) as follows: (a) the protein consisting of an amino acid sequence shown by a sequence 1 in a sequence table; and (b) the protein which is derived from the sequence 1 by performing substitution and / or deletion and / or addition on the amino acid sequence of the sequence 1 with one or more amino acid residues and is related to resistance to plant yellow dwarf. The invention also discloses the coding gene of the protein, as well as a recombinant expression vector containing the gene. After the coding gene is transferred, the disease resistance of susceptible wheat is greatly improved. The study lays a solid foundation for carrying out study on molecular mechanism of wheat resistance to yellow dwarf and molecular breeding and efficiently cultivating novel varieties of wheat which is resistant to yellow dwarf, high in yield and excellent in quality.

A cultivator for efficient clearing of overgrown land to prepare for planting, especially suited to operating in remote, or hard-to-maneuver-in areas, including towing by a light vehicle, comprises a frame interconnecting to hold a drum with protruding gusseted rods at right angles to the forward movement of the frame. A pair of wheels is mounted to the frame such that when the frame is flipped over, the wheels keep drum and rods from contacting ground and vegetation. A height-adjustable, removable grader bar is connected between the wheels. A trailer hitch is connected to the frame by a removable pin, reversible for towing the cultivator with the drum and rods engaging the ground for land clearing and preparation for planting, or alternatively with wheels engaging the ground for leveling ground with the grader bar or transportation. The drum's weight can vary by filling with solids or fluids.

The invention relates to a method for culturing in vitro tissues of Semiliquidambar cathayensis. The method provided by the invention comprises the following steps of: taking Semiliquidambar cathayensis branches of the current year as explants; carrying out a disinfecting treatment on the explants to obtain sterile materials; culturing the sterile materials to form adventitious buds through bud induction; then carrying out a propagation culture, a rooting culture and a domestication and transplantation of tissue culture seedlings to establish a complete rapid-propagation system of the Semiliquidambar cathayensis. By utilizing the method provided by the invention, a lot of Semiliquidambar cathayensis tissue culture seedlings can be simply and efficiently cultured, so that the specie is effectively protected and propagated; the market requirements on the Semiliquidambar cathayensis are satisfied, and the endangered state of the Semiliquidambar cathayensis is eliminated, and the method lays a good foundation on further protecting and utilizing the specie in future.

The invention discloses an efficient controlled release fertilizer favorably improving taste and freshness of ivy mosses. The fertilizer comprises the following components in parts by weight: oyster shells, polylactic acid, expanded graphite, thermally activated nano chernozem, pinecones, pomegranate barks, mosses, sugarcane ash, a soil beneficial microbial inoculant, bacitracin zinc, phytase, amino acid, ground calcium ammonium sulfate, boric acid, sodium selenite, chelated iron, potassium fulvate, lysine, a proper amount of hydrogen peroxide, a proper amount of ammonia water, a proper amount of water and the like. The coated controlled release fertilizer is prepared by using the pinecones, the pomegranate barks, the mosses and the sugarcane ash which are matched with nutritional substances through fermentation. The fertilizer is rich in nutrient elements such as fermented small molecular organic matters, calcium, iron, selenium and zinc, is abundant in nutrient and convenient for plants to absorb and utilize, so that the taste and freshness of the ivy mosses can be enhanced; by adopting the coating layer which can supply oxygen and dynamically adjust the porosity, the fertilizer efficiency time is long, organic matter nutrients can be released for a long time, efficient cultivation of the ivy mosses can be realized, and the fertilizer can be widely applied and popularized.

The invention belongs to the field of molecular breeding, and relates to a molecular breeding method for breeding a new anti-verticillium cotton seed. The method comprises the following steps: taking a chromosome segment introgression line 1L095 of sea-island cotton of which the collection number is CGMCC No.5574 as a female parent and a chromosome segment introgression line 1L154 of sea-island cotton of which the collection number is CGMCC No.5575 as a male parent to crossbreed a generation I seed, and obtaining F2 from the crossbred generation I seed, wherein each generation is collected bya single strain; and selecting by taking a molecular marker for assistance to obtain a novel multi-strain prevention cotton strain. Through the adoption of the method, the novel multi-strain prevention cotton strain-Nannong haidao V is bred. As the molecular marker is taken for assistance to select a target trait, the novel multi-strain prevention cotton strain can be bred in 2 to 3 years fast and effectively. The corresponding disease index of Nannong haidao V is 15.51 to 20.97 percent.

The invention relates to a method for rapidly and selectively culturing a cold-resistant high-quality king rice variety in a rice growing region of Heilongjiang. The method comprises the following technological steps of: by utilizing a high-quality gene variety containing medium amylose content, flexible gel consistency and fragrance as a donor parent, and a main cultivar variety of Heilongjiang as a receptor recurrent parent, performing one-generation hybridization and one-generation backcrossing so as to obtain BC1F1; performing SSR analysis on genetic background of BC1F1 single plants, screening out a plant which contains target genes and more receptor genetic backgrounds to backcross with the recurrent parent so as to obtain BC2F1; obtaining BC3F1 by use of a same method; performing SSR analysis on genetic background of BC3F1 single plants, screening out a plant which contains target genes and more receptor genetic backgrounds, and bagging for selfing, thereby obtaining BC3F2; performing SSR analysis on genetic background of BC3F2 single plants, screening out a plant which contains homozygous target genes and more receptor genetic backgrounds, meanwhile performing cold-resistant screening and identification by use of a 72-hour cold water pooling method before about 15 days of head sprouting, and performing rice quality detection and identification in a laboratory.

The invention belongs to the field of vegetable fertilizer, and more specifically relates to a modified choline chloride high-efficiency fertilizer helpful for increasing of henry steudnera tuber yield. The modified choline chloride high-efficiency fertilizer comprises, by weight, choline chloride, an amphiphilic ionic polypeptide, chitosan modified citric acid, saccharomycetes, fern root residue, furfural residue, nano-selenium, sodium humate, chitosan modified zeolite powder, corn starch, and an appropriate amount of mineralized small molecule water. According to the invention, choline chloride small molecule processing is adopted, and treatment with the amphiphilic ionic polypeptide and chitosan modified zeolite powder is adopted, so that a modified choline chloride high efficiency factor convenient for absorption and utilization is obtained; the modified choline chloride high efficiency factor is mixed with mineralized small molecule water treated nutritional solution, modified saccharomycetes, and compost main materials including fern root residue, furfural residue, and corn starch, so that full absorption and utilization of henry steudnera tuber on fertilizer nutrients are realized, henry steudnera tuber growth is promoted, and henry steudnera tuber high efficiency cultivation is realized.