Sebacinales sp. LQcapable of promoting germination of seeds of medicinal Dendrobium officinale andDendrobium flexicauleto form seedlings and application thereof
A seed and strain technology, applied in the field of applied microorganisms, can solve the complex and cumbersome problems of effective fungal screening and separation, and achieve the effect of simple and convenient separation method, simple process and low cost
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Embodiment 1
[0038] Example 1: Isolation of Sebacinales sp. LQ strain
[0039] (1) Acquisition of protocorms: In September 2018, 24 protocorms of Dendrobium candidum and 3 protocorms of Dendrobium curved stems that germinated naturally were collected in the original habitats of Dendrobium candidum and Dendrobium curved stem in Luotian, Chongqing, and wrapped with moist moss bring back;
[0040] (2) Induction, separation, purification and preservation of symbiotic fungi in protocorms: take out the germinated protocorms of Dendrobium officinale and / or protocorms of Dendrobium curved stems, put them into sterilized beakers filled with 1% sodium hypochlorite (NaClO) solution respectively, Gently shake the beaker, take it out with sterilized blunt-tipped tweezers after 5 minutes, and rinse it with sterile water for 3 to 4 times; cut the protocorm of Dendrobium candidum and / or the protocorm of Dendrobium candidum with a sterile blade on an ultra-clean workbench. Cut into two halves, place them ...
Embodiment 2
[0047] Embodiment 2: Experiment of the effectiveness of promoting the symbiotic germination of Dendrobium candidum LQ bacterial strain:
[0048] The symbiotic germination experiment between seeds and fungi in the medium was used to detect whether the isolated fungi had a promoting effect on the seed germination stage and to compare the differences in the promoting effects of different fungi on the seed germination stage:
[0049] (1) Prepare symbiotic germination medium: the symbiotic germination medium is oat agar medium, including 4g L -1 Oat flour and 8g·L -1 Agar, the pH of the medium is 5.6-5.8; prepare 360 plates of oat medium for sterilization, and there are 6 treatment groups in total;
[0050] (2) Take out the Sebacinales sp.LQ which is stored in the slant of the test tube at a temperature of 4°C, inoculate it on the PDA medium, then place it in an artificial climate box, and cultivate it at a temperature of 25±2°C Activation until the fungal hyphae cover the petr...
Embodiment 3
[0059] Embodiment 3: Experiment of the effectiveness of promoting the symbiotic germination of Dendrobium bentrobii by the LQ strain of O. cereus:
[0060] The symbiotic germination experiment between seeds and fungi in the medium was used to detect whether the isolated fungi had a promoting effect on the seed germination stage and to compare the differences in the promoting effects of different fungi on the seed germination stage:
[0061] (1) Prepare symbiotic germination medium: the symbiotic germination medium is oat agar medium, including 4g L -1 Oat flour and 8g·L -1 Agar, the pH of the medium is 5.6-5.8; prepare 360 plates of oat medium for sterilization, and prepare 30 plates for each experiment, 12 treatments in total;
[0062] (2) Take out the Sebacinales sp.LQ which is stored in the slant of the test tube at a temperature of 4°C, inoculate it on the PDA medium, then place it in an artificial climate box, and cultivate it at a temperature of 25±2°C Activation unt...
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