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The fungus of the genus Glycobacterium and its application in promoting the germination of Paphiopedilum albicans seeds to form seedlings

A technology of film bacteria and fungi, which is applied in the field of applied microorganisms, can solve the problems of uncertain whether the effective symbiotic fungi are the same, cumbersome, complicated screening and separation of effective fungi for seed germination, etc., and achieves a simple and convenient separation method, simple process, and no organization. The effect of the degradation problem

Active Publication Date: 2022-03-08
YUNNAN UNIV
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AI Technical Summary

Problems solved by technology

At present, effective fungi are mostly obtained by isolating from the roots of wild plants of Orchidaceae, but because there are a large number of endophytic fungi with unknown functions in the roots of Orchidaceae, this makes the screening and isolation of effective fungi for seed germination complicated and cumbersome. , meanwhile, whether the fungi in the roots of different orchids are the same as the effective symbiotic fungi at the seed germination stage is still uncertain

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 2

[0064] Example 2: Experiment on the effectiveness of promoting the symbiotic germination of P. albicans seeds by the fungus GYBQ01 strain of the genus Gleomyces

[0065] Use the symbiotic germination experiment of seeds and fungi in the medium to detect whether the isolated fungi have a promoting effect on the seed germination stage and compare the differences in the promoting effects of different fungi on the seed germination stage:

[0066] (1) Prepare symbiotic germination medium: the symbiotic germination medium is oat agar medium, including 4 g·L -1 oat flour and 8 g·L -1 Agar, the pH of the medium is 5.6~5.8; prepare 100 plates of oat medium for sterilization, and each experimental treatment repeats 30 plates, a total of 3 treatments;

[0067] (2) The fungus strains of the genus Colloidal genus that will be stored at a temperature of 4°C on the inclined plane of the test tube Tulasnella sp.GYBQ01 was taken out, inoculated on PDA medium, then placed in an artificial cl...

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Abstract

The present invention relates to fungus of the genus Glycobacterium and the application thereof for promoting the germination of Paphiopedilum japonica seeds to form seedlings. Tulasnella sp.GYBQ01, its deposit number in China Center for Type Culture Collection is: CCTCC NO: M 2020129. The invention provides the morphology and physiological and biochemical characteristics of the bacterial strain. According to the results of morphological, physiological, biochemical and nucleotide sequence analysis, Tulasnella sp.GYBQ01 belongs to a fungus belonging to the genus Pleurotus genus of the family Glycobacteriaceae. This strain can be used to promote the germination of P. chinensis seeds to form seedlings. It has strong specificity. It can be used to inoculate P. Symbiotic seedlings, high-efficiency cultivation of seedlings to achieve symbiotic germination, can be used for seedling cultivation in nurseries or field fungal seed dressing and direct seeding. Compared with tissue culture, it has the advantages of low cost, high survival rate in field cultivation, and no tissue degradation. It lays the foundation for the imitation ecological cultivation, species regression and population reconstruction.

Description

technical field [0001] The invention relates to a fungus of the genus Glycophaga and the application thereof for promoting the germination of Paphiopedilum papillosa seeds to form seedlings, and belongs to the field of applied microorganisms. Background technique [0002] Paphiopedilum alba ( Paphiopedilum spicerianum ), was first found in Assam in northern India, and later in northeastern India along the border with Myanmar. The sepals in the flowers are large and white, like a white flag, the upper part is curved forward into an arch, and the pseudostamens are ear-shaped and heart-shaped, which has high ornamental and breeding value. In 2006, in Pu'er City, Yunnan Province, my country, the wild distribution of P. albicans was discovered for the first time, and it is also the only confirmed wild distribution point in my country. There are only more than 20 plants in the original population. This species grows on the steep bank of a small river ditch at the entrance of an ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12N1/02A01G31/00A01G7/06C12R1/645
CPCC12N1/02A01G31/00A01G7/06C12R2001/645C12N1/145
Inventor 高江云杨文科王新菊
Owner YUNNAN UNIV
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