Fungus of tulasnellaceae and application of fungus to promotion of seed germination and seedling forming of paphiopedilum spicerianum

Abstract

The invention relates to a fungus of tulasnellaceae and an application of the fungus to promotion of seed germination and seedling forming of paphiopedilum spicerianum. The fungus is a strain of tulasnellaceae and is Tulasnellaceae sp. GYBQ02, and the collection number of the fungus in China Center for Type Culture Collection is CCTCC NO:M 20203130. The invention provides morphology and physiological and biochemical characteristics of the strain. According to morphological, physiological and biochemical and nucleotide sequence analysis results, Tulasnellaceae sp. GYBQ02 is the strain of tulasnellaceae, and the strain can be used for promoting germination of paphiopedilum spicerianum seeds to form seedlings, has high specificity, can be used for inoculating paphiopedilum spicerianum seeds to obtain high-quality symbiotic seedlings, and high-efficiency breeding of symbiotic germination is achieved; the strain can be used for nursery seedlings or field fungus dressing and direct seeding,and has the advantages of low cost, high field culture survival rate, no tissue degradation and the like, as compared with tissue culture; and a foundation is laid for bionic culture, species return and population reconstruction of paphiopedilum spicerianum.

Description

technical field [0001] The invention relates to a fungus of the family Glycobacteriaceae and the application thereof for promoting the germination of Paphiopedilum japonica seeds to form seedlings, and belongs to the field of applied microorganisms. Background technique [0002] Paphiopedilum alba ( Paphiopedilum spicerianum ), was first found in Assam in northern India, and later in northeastern India along the border with Myanmar. The sepals in the flowers are large and white, like a white flag, the upper part is curved forward into an arch, and the pseudostamens are ear-shaped and heart-shaped, which has high ornamental and breeding value. In 2006, in Pu'er City, Yunnan Province, my country, the wild distribution of P. albicans was discovered for the first time, and it is also the only confirmed wild distribution point in my country. There are only more than 20 plants in the original population. This species grows on the steep bank of a small river ditch at the entrance...

AI Technical Summary

Problems solved by technology

At present, effective fungi are mostly obtained by isolating from the roots of wild plants of Orchidaceae, but because there are a large number of endophytic fungi with unknown functions in the roots of Orchidaceae, this makes the screening and isolation of effective fungi for seed germination complicated and cumbersome. , meanwhile, whether the fungi in the roots of different orchids are the same as the effective symbiotic fungi at the seed germination stage is still uncertain