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Method for culturing in vitro tissues of Semiliquidambar cathayensis

A tissue culture, semi-maple lotus technology, applied in the field of plant tissue culture, to meet the needs and relieve the endangered state.

Active Publication Date: 2012-01-04
盐城欧雅居木业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the prior art, the callus is induced by the leaves of the maple lotus as explants, and the callus is proliferated, and then induced to differentiate into buds, and no adventitious roots are induced.

Method used

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  • Method for culturing in vitro tissues of Semiliquidambar cathayensis
  • Method for culturing in vitro tissues of Semiliquidambar cathayensis
  • Method for culturing in vitro tissues of Semiliquidambar cathayensis

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Embodiment

[0029] Embodiment: the tissue culture of Banfenghe

[0030] 1. From the collection of the maple lotus to the induction of germination

[0031] After a week of continuous sunny days, the materials were collected at 12-14 noon, and the current-year branches of the plants propagated from the seeds of the excellent mother plant in Tianlong Mountain, Quannan County, Jiangxi Province were used as explants. They were washed with detergent and soaked for 10 minutes, and then rinsed with running water. Rinse for 30 minutes, place on the operating table, cut into stems with 1-2 internodes, treat with 75% ethanol for 25 seconds, soak in 0.1% mercury chloride for 10 minutes, shake continuously during the period, wash with sterile water 6 times, inoculate On the bud induction medium, the light time is 8~12 hours, the light intensity is 3000~5000lx, the temperature is 25~27℃, after 14 days, axillary buds of 2~3cm can be induced, see the attached figure 1 and 2 . The induction rate is 7...

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Abstract

The invention relates to a method for culturing in vitro tissues of Semiliquidambar cathayensis. The method provided by the invention comprises the following steps of: taking Semiliquidambar cathayensis branches of the current year as explants; carrying out a disinfecting treatment on the explants to obtain sterile materials; culturing the sterile materials to form adventitious buds through bud induction; then carrying out a propagation culture, a rooting culture and a domestication and transplantation of tissue culture seedlings to establish a complete rapid-propagation system of the Semiliquidambar cathayensis. By utilizing the method provided by the invention, a lot of Semiliquidambar cathayensis tissue culture seedlings can be simply and efficiently cultured, so that the specie is effectively protected and propagated; the market requirements on the Semiliquidambar cathayensis are satisfied, and the endangered state of the Semiliquidambar cathayensis is eliminated, and the method lays a good foundation on further protecting and utilizing the specie in future.

Description

technical field [0001] The invention relates to a plant tissue culture method, in particular to a semi-maple lotus tissue culture method. Background technique [0002] Half maple lotus Altingia chingii Mete is an evergreen tree of the family Hamamelis, a national second-class protected plant. Endemic to China, it has the comprehensive traits between the two genera of Liquidambar Liguidambar and Altingia, and has scientific value for the study of Hamamelidaceae phylogeny. The wood material is excellent, and the rotary planer is good, so it can be used as rotary planer products. At the same time, the bark has the effects of relaxing tendons, activating blood circulation, expelling wind and dehumidification, etc. It is used in the folk to treat rheumatoid arthritis, chronic low back pain, hemiplegia, bruises and other diseases, and the curative effect is remarkable. Due to the serious disturbance of natural forests and the difficulty of natural reproduction, the distributio...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 林文革陈细萍曹冬萍刘秀芳徐进芳
Owner 盐城欧雅居木业有限公司
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