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Method for rapidly and selectively culturing cold-resistant high-quality king rice variety in rice growing region of Heilongjiang

A cold-resistant and rapid technology, applied in the fields of botanical equipment and methods, biochemical equipment and methods, and microbial determination/inspection, can solve problems such as low efficiency, difficult control, and large environmental impact, and achieve enhanced cold resistance and high efficiency. The effect of breeding and retaining the genetic background of the recipient

Inactive Publication Date: 2016-06-22
国家粳稻工程技术研究中心 +1
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Problems solved by technology

[0004] The conventional method of rice breeding for eating quality is inefficient, and rice quality is a comprehensive expression of multiple traits, and each trait is difficult to control, and is greatly affected by the environment. Accelerating the selection process of high-quality rice and improving the breeding efficiency of high-quality rice are important problems that need to be solved urgently in rice quality breeding

Method used

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Examples

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Effect test

example 1

[0012] Example 1 Using Daohuaxiang as the donor parent and Longjing 25 as the recipient, an improved line of Longjing 25 with high quality and cold tolerance was bred.

[0013] (1) The variety Daohuaxiang containing medium amylose content, soft gel consistency, and good quality genes of fragrance was used as the donor parent, and Longjing 25 with strong cold tolerance was used as the recipient recurrent parent, and the two were crossed to obtain The first generation of hybrids is backcrossed with the recipient parents. In order to ensure the integrity of the genotype, the method of "multiple holes and few seeds" is adopted to obtain BC 1 f 1 200 capsules of the first generation of backcross;

[0014] (2) to BC 1 f 1 Perform SSR detection on a single plant, and screen the single plant that contains the target genes of medium amylose content, soft gel consistency, and fragrance, and has the most identical bands with the recurrent parent, and then backcross with the recurrent ...

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Abstract

The invention relates to a method for rapidly and selectively culturing a cold-resistant high-quality king rice variety in a rice growing region of Heilongjiang. The method comprises the following technological steps of: by utilizing a high-quality gene variety containing medium amylose content, flexible gel consistency and fragrance as a donor parent, and a main cultivar variety of Heilongjiang as a receptor recurrent parent, performing one-generation hybridization and one-generation backcrossing so as to obtain BC1F1; performing SSR analysis on genetic background of BC1F1 single plants, screening out a plant which contains target genes and more receptor genetic backgrounds to backcross with the recurrent parent so as to obtain BC2F1; obtaining BC3F1 by use of a same method; performing SSR analysis on genetic background of BC3F1 single plants, screening out a plant which contains target genes and more receptor genetic backgrounds, and bagging for selfing, thereby obtaining BC3F2; performing SSR analysis on genetic background of BC3F2 single plants, screening out a plant which contains homozygous target genes and more receptor genetic backgrounds, meanwhile performing cold-resistant screening and identification by use of a 72-hour cold water pooling method before about 15 days of head sprouting, and performing rice quality detection and identification in a laboratory.

Description

technical field [0001] The invention proposes a method for cultivating new varieties of rice with molecular marker-assisted selection, rapid enhancement of cold resistance and improvement of eating quality. It only takes 5 generations to control the rice medium amylose content, soft gel consistency, and aroma genes to be directed into the recipient variety, while retaining other characteristics of the recipient variety. The procedures and steps of cultivating high-quality new rice varieties are adopted by combining hybridization, backcrossing, molecular marker detection and rice quality analysis. While ensuring the introduction of the target gene of the donor, more of the genetic background of the recipient is retained, and the improved high-quality New varieties can be bred efficiently. Background technique [0002] The main goals of rice improvement in Heilongjiang rice region are cold resistance, high yield, high quality, and multi-resistance. However, with the continuo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02A01H1/04C12Q1/68
CPCA01H1/02A01H1/04C12Q1/6895C12Q2600/156
Inventor 朱崴李志彬刘桂林亓娜章莹蔡卓曲丽君肖艳云关峰刘欣
Owner 国家粳稻工程技术研究中心
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