Method for rapidly and selectively culturing cold-resistant high-quality king rice variety in rice growing region of Heilongjiang
A cold-resistant and rapid technology, applied in the fields of botanical equipment and methods, biochemical equipment and methods, and microbial determination/inspection, can solve problems such as low efficiency, difficult control, and large environmental impact, and achieve enhanced cold resistance and high efficiency. The effect of breeding and retaining the genetic background of the recipient
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[0012] Example 1 Using Daohuaxiang as the donor parent and Longjing 25 as the recipient, an improved line of Longjing 25 with high quality and cold tolerance was bred.
[0013] (1) The variety Daohuaxiang containing medium amylose content, soft gel consistency, and good quality genes of fragrance was used as the donor parent, and Longjing 25 with strong cold tolerance was used as the recipient recurrent parent, and the two were crossed to obtain The first generation of hybrids is backcrossed with the recipient parents. In order to ensure the integrity of the genotype, the method of "multiple holes and few seeds" is adopted to obtain BC 1 f 1 200 capsules of the first generation of backcross;
[0014] (2) to BC 1 f 1 Perform SSR detection on a single plant, and screen the single plant that contains the target genes of medium amylose content, soft gel consistency, and fragrance, and has the most identical bands with the recurrent parent, and then backcross with the recurrent ...
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