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Whole-genome-selection-based method for breeding improved disease-resistant fish varieties

A technology of whole genome and breeding method, which is applied in the field of fish disease-resistant breeding, can solve the problems of lack of whole-genome selection methods, and achieve the goal of improving disease resistance and breeding survival rate, wide application value and promotion prospect, and high-efficiency breeding Effect

Active Publication Date: 2017-03-08
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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Problems solved by technology

[0005] The purpose of the present invention is to establish a method for cultivating fish disease-resistant improved varieties based on whole genome selection, to make up for the shortcomings of traditional breeding techniques, to provide molecular breeding methods for fish disease-resistant, high-yield, and high-quality improved varieties, and to solve the lack of comprehensive genetic information in the fish farming industry. The problem of genome selection method provides the technical means of breeding disease-resistant and excellent varieties for the fish farming industry, realizes the upgrading of fish breeding technology, and promotes the rapid development of the fish seed industry

Method used

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  • Whole-genome-selection-based method for breeding improved disease-resistant fish varieties

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Embodiment Construction

[0035] Taking flounder and half-smooth tongue sole as examples, the breeding method of fish disease-resistant improved species based on whole-genome selection will be described in detail in conjunction with the accompanying drawings:

[0036] 1. Establishment of fish disease-resistant reference populations and determination of phenotypic traits

[0037] When the established fish family fries grow to a total length of 8-15 cm, pathogenic microorganisms are used to artificially infect the fish family fry with pathogenic bacteria, and the dead fry of each family are collected at different times after the onset of pathogenic bacteria infection, and the death time and time of the fish fry are recorded. Phenotypic data such as full length, weight, and body width, while collecting surviving individuals after infection and recording the data of full length, weight, and body width, and using the collected data of surviving individuals and dead individuals as phenotypic parameters for ev...

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Abstract

The invention aims at providing a whole-genome-selection-based method for breeding improved disease-resistant fish varieties, thereby overcoming defects in the traditional breeding technology, providing a molecular breeding method for cultivation of improved disease-resistant high-yield fish varieties, solving a problem of lack of a whole-genome selection method in the existing fish breeding industry, providing a technical means for improved disease-resistant fish variety breeding of the fish breeding industry, realizing upgrading of the fish breeding technique, and promoting rapid development of the fish breeding industry. The disease-resistant fish offspring obtained by using the method provided by the invention has the enhanced disease-resistant capability. An experiment result demonstrates that the survival rate from infection and breeding survival rate of a disease-resistant fish offspring that is bred based on the whole genome selection method are higher than those of the control group by 20% to 30%. Using the method provided by the invention, the improved disease-resistant fish breed can be bred rapidly and efficiently; and the disease-resistant capability and breeding survival rate of fish can be enhanced. Therefore, the method provided by the invention has the great application value and broad promotion prospects in the fish breeding industry.

Description

technical field [0001] The invention belongs to the technical field of aquatic genetics and breeding, and in particular relates to a method for cultivating improved disease-resistant fish species based on whole genome selection. Background technique [0002] Fish farming is the pillar industry of my country's aquaculture industry. In 2014, the fish farming output was 27.2194 million tons, accounting for 57.3% of the entire aquaculture output. Farmed fish is an important source of food protein in my country. [0003] However, with the rapid development of the fish farming industry, the lack of good varieties and the degradation of the germplasm of the cultured species are prominent; the expansion of the breeding scale, the improvement of the degree of intensification and the deterioration of the breeding environment have caused frequent occurrence of aquaculture diseases, and the aquaculture products Problems such as outstanding drug residues seriously restrict the sustainab...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68A01K61/13G06F19/18
CPCA01K61/00C12Q1/6888C12Q2600/156G16B20/00Y02A40/81
Inventor 陈松林刘洋刘峰李仰真邵长伟王磊王娜周茜刘寿堂翟介明郑卫卫张英平孙河军杨英明
Owner YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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