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Breeding method for densovirus disease resistant and fluorine resistant cultivated silkworm breed variety

A technology of dense nuclear disease and fluorine resistance, which is applied in the field of silkworm breeding, to achieve the effect of heavy workload, long breeding cycle and poor selection reliability

Inactive Publication Date: 2011-06-01
JIANGSU UNIV OF SCI & TECH
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Problems solved by technology

[0006] Technical problem: The purpose of the present invention is to solve the technical problem of using molecular markers in assisted breeding selection of silkworms, and to provide a breeding method for breeding silkworms resistant to dense nuclear disease and fluorine-resistant by using molecular markers assisted selection method

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Embodiment Construction

[0020] 1. Description of the materials used

[0021] Existing research results have shown that the non-infection of silkworm to densokaryophysis Zhenjiang strain is controlled by the recessive single gene nsd-Z (complete resistance), and only in the case of gene homozygosity can it show non-infection to densoplasmosis Zhenjiang strain (Complete resistance) (Hu Xuefang, Qian Yuanjun, Wang Honglin. Infection Resistance of Bombyx mori Densovirus (DNV) to Different Silkworm Species. Sericulture Science, 1984, 10(2): 87~90); The tolerance of fluoride is controlled by the dominant main effect gene Def (the fluoride resistance threshold is 200mg / kg) (Lin Changlin, Mi Yidian, Yao Qin, etc. The discovery of the main effect gene of fluoride resistance in silkworm, Sericulture Science, 1997, 23 (4):237-239), as long as the gene is present, whether heterozygous or homozygous, it shows tolerance to fluoride.

[0022] (1), the parent silkworm variety Lan 10 (known and public, "Chinese Silk...

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Abstract

The invention discloses a breeding method of anti-densonucleosis and fluorin-resistant solkworn strain. The breeding method uses the Chinese race 'Jiang Song' and the Japanese race 'Hao Yue' as recurrent parents and the polyvoltine race Lan10 is used as the anti-densonucleosis high-grade object character donor parent and the T6 is used as the fluoride-resistant high-grade object character donor parent. The first hybridization generation obtained by hybridizing the recurrent parents with the Lan10 is hybridized with the T6 and then the obtained filial generation is continuously backcrossed with the recurrent parents and each generation is subjected to individual selection during breeding and the selected individual is marked by molecule to select the genotype thereof two anti-densonucleosis and fluorin-resistant silkworn strains 'JsFnsd-Z' and 'HyFnsd-Z' are selected after self-cross homozygosis for six generations. The 'JsFnsd-Z' and 'HyFnsd-Z' have strong physical constitution and dons not infected by the Bombyxmori densovirus and the whole-instars fluoride-resistance rate is above 100mg / kg and the fluoride-resistance performance is higher than the Chinese race 'Jiang Song' and the Japanese race 'Hao Yue' by 50% and the imago fin has no palea scale, in addition the practical character corresponds with the utility breed level.

Description

technical field [0001] The invention relates to a breeding method for silkworm varieties, more specifically, relates to a breeding method for breeding silkworm varieties resistant to dense nuclear disease and fluorine resistance by using molecular marker assisted selection method. technical background [0002] Sericulture and silk reeling have a history of more than 5,000 years in my country. It is one of the most distinctive traditional advantageous industries in my country and one of the few products that can dominate the international market. At present, my country's silkworm cocoon and raw silk production ranks first in the world First, they respectively account for about 75% of the world's total output. [0003] The silk industry not only plays an important role in increasing farmers’ income and expanding labor employment, but also the food of silkworms—mulberry trees also play an important role and significance in environmental protection and returning farmland to fores...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K67/04
Inventor 李木旺徐安英侯成香孙平江张月华钱荷英郭锡杰
Owner JIANGSU UNIV OF SCI & TECH
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