Primer for sex identification of Nile tilapia and PCR (polymerase chain reaction) identification method

Abstract

The invention discloses a primer for sex identification of Nile tilapia. The primer can specifically amplify AMH genes of Nile tilapia, a male individual generates two specifically amplified segments, lengths of the specifically amplified segments are 179bp and 412bp respectively, and a female individual generates a specifically amplified segment of 412bp in length. The primer is used to amplify genome DNA of Nile tilapia, the male individual generates the two specifically amplified segments of 179bp and 412bp in length, the female individual generates the specifically amplified segment of 412bp in length, and the male individual and the female individual can be distinguished obviously, so that sexes of Nile tilapia can be identified simply and accurately. The primer is high in specificity and does not amplify other segments except for a target segment. The invention further discloses a PCR (polymerase chain reaction) identification method using the primer. The PCR identification method is quick to operate and accurate in result, and the defect of unstable results caused by adopting conventional morphological methods to identify Nile tilapia different in sex is avoided.

Description

technical field [0001] The invention relates to biological detection and identification technology, in particular to a primer for PCR identification of Nile tilapia sex and a PCR identification method using the primer. Background technique [0002] Nile tilapia belongs to Perciformes, Cichlidae, and Tilapia genus. It is native to Lake Tanganyika in Jordan. It was introduced and promoted in my country from Thailand in 1978. Now my country is the largest tilapia in the world. Non-fish producing and exporting countries. The main species of tilapia cultured in my country are Nile tilapia, Oria tilapia and Oni hybrid tilapia. Due to the large overlap of morphological characteristics among different species of tilapia, it is very difficult to distinguish different tilapia species only based on their morphology. In addition, it is easy to hybridize between tilapia varieties, and the hybrids are fertile, and the hybrids are similar in shape to their parents. Therefore, it is easy t...

AI Technical Summary

Problems solved by technology

Due to the large overlap of morphological characteristics between different species of tilapia, it is very difficult to distinguish different tilapia species based on their morphology alone.

In addition, it is easy to hybridize between tilapia varieties, and the hybrids are fertile, and the hybrids are similar in shape to their parents. Therefore, it is easy to cause tilapia germplasm to mix, leading to the degradation of good economic traits, which is currently restricting our country. Prominent Problems in the Development of Tilapia Aquaculture Industry

During the breeding process, the growth of male and female tilapias is significantly different, and the male fish grows faster than the female fish, which often leads to a large difference in the size of the pond, thereby reducing the quality of commercial fish and reducing economic benefits.

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