SNP primers for early screening strongylocentrotus intermedius fine variety and screening method

Abstract

The invention discloses SNP primers for early screening a strongylocentrotus intermedius fine variety and a screening method. The screening method comprises the following steps: selecting a strongylocentrotus intermedius second-generation male individual (Mars symbol) self-bred in Japan and a female individual (Venus symbol) bred for more than five generations in Longwangtang, Lvshun, China as male and female parents to crossbreed an F1 generation; establishing a mapping population containing 194 F1-generation individuals and two parents; cultivating in the same environment, extracting DNA, sequencing and taking other technical measures to find genetic markers of strongylocentrotus intermedius for the first time and design the relevant primers, wherein the primers can be used for early screening the strongylocentrotus intermedius fine variety. Compared with the prior art, the screening method has the advantages as follows: by the screening method, not only can manpower and material resources be saved and the seed selection be reduced, but also good genetic genes of the parents can be ensured, so that good progeny traits can be ensured.

Description

technical field [0001] The invention relates to a primer and a method for screening improved sea urchin intermedia, in particular to a SNP primer and a screening method for early screening improved sea urchin intermedius that can save manpower and material resources, reduce selection costs, and ensure good progeny traits. Background technique [0002] At present, traditional breeding methods such as crossbreeding and selective breeding are mostly used for the breeding of sea urchins intermedia. No matter it is crossbreeding or selective breeding, the parents are selected based on sea urchins with good growth characteristics. In this way, during the growth process of sea urchins, it is necessary to measure the shell height, shell diameter and live weight of sea urchins on a regular basis (such as every month, etc.), so as to carry out step-by-step screening, which not only consumes a lot of manpower and material resources, but also has all kinds of problems. The problem that ...

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Problems solved by technology

In this way, during the growth process of sea urchins, it is necessary to measure the shell height, shell diameter and live weight of sea urchins regularly (such as every month, etc.), so as to carry out step-by-step screening, which not only consumes a lot of manpower and material resources, but also has all kinds of problems The selection of the parental genetics is not good, resulting in a low yield of offspring seedlings with good genotypes

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