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SNP primers for screening takifugu rubripes seedlings and screening method

A technology for puffer and puffer seed, which is applied in the field of screening primers for puffer puffer, can solve the problems of manpower and material resources, long cycle, poor genetic genes of parents, etc., so as to reduce the cost of selection and save manpower Material effect

Active Publication Date: 2016-11-23
DALIAN OCEAN UNIV
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Problems solved by technology

[0003] The traditional breeding method for fish fry is the phenotypic selection method, that is, to select pufferfish with good growth characteristics such as body weight, body length and body length as parents, which requires regular testing and step-by-step screening, which not only consumes a lot of manpower Material resources, long cycle, low efficiency, and the problem that the genetic genes of the selected parents are not good

Method used

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  • SNP primers for screening takifugu rubripes seedlings and screening method
  • SNP primers for screening takifugu rubripes seedlings and screening method
  • SNP primers for screening takifugu rubripes seedlings and screening method

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Embodiment Construction

[0016] 1. Acquisition of SNP markers related to growth traits of puffer puffer

[0017] 1.1 Acquisition of redfin puffer populations

[0018] The population used is one of the families of red fin puffer hatched by a red fin puffer farm in Dalian. 300 individuals were randomly selected from this family, and the weight, body length and total body length of these 300 individuals were firstly measured. The phenotypic values ​​of growth traits were measured and recorded, and then 100 fish caudal fin rays were cut and stored in 80% ethanol, and stored in a -20°C refrigerator for the extraction of genomic DNA.

[0019] 1.2 Genomic DNA extraction of redfin puffer

[0020] The genomic DNA in the fin rays of puffer puffer was extracted by the conventional phenol imitation method, and the specific steps were as follows:

[0021] (1) Take 100~120 mg of the caudal fin tissue of puffer puffer in a 1.5 ml centrifuge tube, and use ophthalmic scissors to cut it as much as possible (in powde...

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Abstract

The invention discloses SNP primers for screening takifugu rubripes seedlings and a screening method, wherein the human and material resources can be saved, the seed selection cost is reduced, and good characters of filial generations are guaranteed. The sequences of the SNP primers are shown as SEQ ID No.1 and SEQ ID No.2; and the seedling screening method comprises the following steps: extracting the genome DNA of the to-be-detected takifugu rubripes; with the obtained genome DNA as a template, performing PCR amplification on the forward primer and the reverse primer to obtain a PCR product; and performing sequencing and genotyping of the obtained PCR product and screening out the takifugu rubripes of which the DNA sequence is shown as SEQ ID No.3 and the genotype of the 145th site from the 5' end is homozygous TT.

Description

technical field [0001] The invention relates to a primer and a method for screening puffer puffer seedlings, in particular to a SNP primer and a screening method for screening puffer puffer puffer seedlings which can save manpower and material resources, reduce seed selection costs, and ensure good traits of offspring. Background technique [0002] Redfin Pufferfish ( Takifugu rubripes ) is an important economic fish for mariculture in northern my country. [0003] The traditional breeding method for fish fry is the phenotypic selection method, that is, to select pufferfish with good growth characteristics such as body weight, body length and body length as parents, which requires regular testing and step-by-step screening, which not only consumes a lot of manpower Material resources, long cycle, low efficiency, and the problem that the genetic genes of the selected parents are not good. [0004] Molecular marker-assisted breeding is one of the most ideal methods to quickl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 王秀利于海龙仇雪梅姜志强孟雪松刘圣聪张涛包玉龙
Owner DALIAN OCEAN UNIV
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