A SNP molecule mark for identifying individual gender of Takifugu rubripes and an identification method

Abstract

The invention discloses a SNP molecule mark for identifying individual gender of Takifugu rubripes. The SNP molecule mark for identifying individual gender of Takifugu rubripes is characterized in that a DNA sequence is shown in SEQ ID NO: 1, and a base N of the 121st position and the 262th position in the sequence is C or G. The identification method perform according to the following steps: a Takifugu rubripes genome DNA is used as a template, PCR amplification is performed by upstream primer and downstream primer, a PCR product is obtained; the DNA sequences of the upstream primer and the downstream primer is respectively shown in SEQ ID NO: 2 and SEQ ID NO: 3; sequencing is performed on the obtained PCR product, the individual that the 121st position and the 262th position of the measured DNA sequence are simultaneously double peaks is a Takifugu rubripes male fish, and the individual that the 121 position and the 262th position of the measured DNA sequence are simultaneously a single peak is a Takifugu rubripes female fish.

Description

technical field [0001] The invention relates to a SNP molecular marker and identification method for identifying the individual sex of red-finned puffer, especially a SNP for identifying the sex of red-finned puffer, which can simultaneously obtain male and female determination results, is easy to operate, and can improve the accuracy of identification Molecular markers and identification methods. Background technique [0002] Redfin Pufferfish ( Takifugu rubripes ) is an important sea-farming economic fish in the northern coast of my country. Its meat is delicious and nutritious, and it is favored by consumers in East Asian countries. The aquaculture output of redfin puffer per year in my country can reach more than 5,000 tons, and the artificial propagation, seedling raising, and growing technologies are basically mature, but there is still a big gap with the needs of the industry in terms of germplasm resources and new species creation. To find out the population resourc...

AI Technical Summary

Problems solved by technology

Takashi Kamiya, et al., Plos Genetics, 2012, 8(7):e1002798, have confirmed that the 7271st locus of the Amhr2 gene of the red fin puffer is the sex determination site of the red fin puffer, but this single site is used to identify the red fin puffer. There is a problem of low accuracy in the individual sex of puffer puffer

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