The invention discloses a
complete sequence amplification method for a mitochondrial
genome of diptychus maculates steindachner. The
complete sequence amplification method specifically comprises the following steps: (1) amplifying four
gene segments Cyt b, COI, ND4 and 16S rRNA of the mitochondrial
genome of the diptychus maculates steindachner; and (2) carrying out LA-PCR amplification on the mitochondrial
genome of the diptychus maculates steindachner. According to the
complete sequence amplification method, the four
gene segments Cyt b, COI, ND4 and 16S rRNA of the mitochondrial genome of the diptychus maculates steindachner are amplified, four pairs of LA-PCR primers are designed according to an obtained sequence and are used for carrying out large-segment sequence amplification, and 28 sequencing primers are utilized for carrying out PCR amplification on the other parts of the mitochondrial genome of the diptychus maculates steindachner by virtue of a primer PCR-Walking method so as to obtain a whole-genome sequence, with a length of 16895bp, of a
mitochondrion of the diptychus maculates steindachner. The inheritance and evolution of the diptychus maculates steindachner are researched in a
molecular level, and particularly mtDNA is used as a
molecular marker, so that the basic data and
technical support are provided for the researches of
species identification,
population genetic structures and
system evolution of the diptychus maculates steindachner.