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A technology of toxicity test and water ecology, which is applied in the direction of testing water, material inspection products, measuring devices, etc.
Active Publication Date: 2019-09-24
SHANGHAI OCEAN UNIV
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However, domestic research on fish toxicity mostly involves the field of freshwater. The fish species used in the test are usually freshwater fish, and the field of seawater medium has not...
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Embodiment 1
[0040] At different concentrations of naphthalene: 0μg / L, 20μg / L, 35.6μg / L, 63.37μg / L, 112.8μg / L, 200μg / L, the changes of water quality conditions during the acute toxicity test of gobies were tested, including pH, dissolution Oxygen, temperature, as shown in Table 1, the acute toxic effects of naphthalene on gobies are as follows figure 1 shown.
[0041] Table 1: Water quality parameters for acute toxicity of naphthalene to fish
[0042]
Embodiment 2
[0044] Under different sodium sulfate concentrations: 0g / L, 3g / L, 4.23g / L, 5.96g / L, 8.41g / L, 12g / L, the changes of water quality conditions during the acute toxicity test of gobies were tested, including pH, Dissolved oxygen, temperature, as shown in Table 2; The acute toxic effect of sodium sulfate on gobies is as follows figure 2 shown.
[0045] Table 2: Water quality parameters for acute toxicity of sodium sulfate to fish
[0046]
[0047]
[0048] According to the calculation of the goby acute toxicity test data of embodiment 1 and 2, it can be obtained: the 96h LC of naphthalene to goby 50 It was 100.23 μg / L, and its 95% confidence interval ranged from 77.77 to 129.15 μg / L. From this data, it can be known that naphthalene is extremely toxic to gobies. 96h LC of Sodium Sulfate on Gobies 50 It is 7.55g / L, and its 95% confidence interval ranges from 7.25 to 8.90g / L. It can be seen from the data that sodium sulfate is of low toxicity to gobies. By measuring the pH va...
Embodiment 3
[0050] After 7 days of the test, the number of gobies survived in the chronic toxicity test of gobies under different naphthalene concentrations: 0μg / L, 20μg / L, 40μg / L, 60μg / L, 80μg / L, and 100μg / L , Body weight and body length changes are shown in Table 3 and Table 4.
[0051] Table 3: Number of surviving gobies
[0052]
[0053] Table 4: Body weight / length changes of gobies
[0054]
[0055]
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Abstract
The invention discloses a marine fish water ecology toxicity test method. The method comprises the following steps of selecting gobies as tested fish species and domesticating in standard water which has a same water quality condition as a tested water sample; preparing a stock solution of the water sample to be tested; selecting a concentration gradient range through acute toxicity and chronic toxicity trial tests of the gobies, setting a concentration gradient with a small difference, taking sea water as a contrast group, and taking the water sample to be tested as a test group; simultaneously, carrying out acute toxicity and chronic toxicity tests of the gobies, recording changes in the gobies and the environment, carrying out variance analysis on data, and drawing a mortality curve of the gobies; and determining water quality toxicity through combining a physical condition of the gobies and water body index changes. Problems that current domestic research on a fish toxicity test is relatively simple, and there is aquatic ecotoxicity test method suitable for a salt-containing condition are solved.
Description
technical field [0001] The invention relates to a marine fish water ecotoxicity test method, in particular to two types of toxicity tests, acute toxicity and chronic toxicity, which are used for detecting marine water ecotoxicity. Background technique [0002] As a traditional method for determining the toxicity of substances, toxicity testing is an experimental method widely recognized by everyone, and it is versatile and repeatable. Its data can be used as the data basis for other related experiments, and it can also be more effective through experimental conclusions. to control the poison. Toxicity tests include: acute toxicity test, chronic toxicity test, genetic toxicity test and endocrine disruption toxicity test. At present, the research on fish toxicity test in China is relatively single, and the most research is on acute toxicity test, which specifically refers to the poisoning effect caused by the body (human or experimental animal) once (or multiple times within ...
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